An experiment was conducted on the relationship among yield, sugar content and enzyme activities and hormone contents in leaves of sugarcane under different fertilizations. The results showed that the physiological characters were different among treatments during different growth stages, the difference were obvious especially in tillering and maturing stage. In tillering stage, IAA, GA 1/3 contents and AIA, NRA were comparatively higher, while the contents of ABA and ZRs were relatively lower. In maturing stage, the contents of IAA, GA 1/3 , ABA and ZRs were fairly higher, while AIA and NRA were relatively lower. During tillering-elongating stage, IAA and GA 1/3 contents increased gradually, ABA content rose a little, while the content of ZRs were decreasing; AIA and NRA were increasing in the mean time. During elongating-maturing stage, the contents of all the four hormones rose gradually, while AIA and NRA descended progressively. Besides, there was a relationship between the activities of enzymes and contents of hormones.