Two techniques, radicel-soaking and seedling root-cutting inoculation, were established according to the effects of disease rate in seedling stage for evaluation of resistance of yardlong bean (Vigna sesquipedalis) to fusarium wilt disease (Fusarium oxsporum f. sp. tracheiphilum). 28 lines were screened for resistance against this pathogen. The results showed that root-cutting inoculation method was suitable for evaluating the resistance of yardlong bean seedling. Among 28 lines, there were 3 resisitant, 12 middle, 8 susceptible and 5 high susceptible. The line Fengchan No2 was the resistant with good pod quality.