Based on the reported sequences of NA gene of avian influenza virus(AIV) H9N2 subtype,a pair of primers was designed for the amplification of NA gene from a strain of AIV H9N2 subtype isolated from a pheasant(A/Pheasant /Guangdong /GZI/2003(H9N2) by RT-PCR.Sequence analysis indicated that the NA gene was 1 458 bp and the open reading frame(ORF) was 1 401 bp in length,encoding for 466 amino acids,sharing more than 98% identities with A/Chicken/Guangdong/11/97(H9N2).