【Objective】 Effects of low temperature on physiological characteristics of maize root at booting stage were studied.【Method】 Xianyu335 and Dongdan213 were selected as the tested materials, and the effects of low temperature on physiological characteristics of maize root at booting stage, and the correlations of all physiological indicators were studied.【Result and conclusion】 The results showed that the activities of SOD, POD, CAT and the contents of Pro, soluble protein and sugar of Xianyu335 were higher than those of Dongdan213 with the prolongation of the low temperature stress time, and MDA content showed the opposite changes. The correlation analysis showed that there was a significant positive correlation between root activity and SOD activity, soluble sugar content in Xianyu335, and there was a significant positive correlation between root activity and CAT activity in Dongdan213. Root activity was positively correlated with the content of Pro, and negatively correlated with MDA content.