【Objective】To understand the grain characteristics of indica rice varieties with national high-quality. 【Method】 Yuejingsimiao2 and other rice varieties were employed to study the grain qualities, including the milling quality, appearance quality, cooking quality, nutritional quality and starch RVA profile. 【Result and conclusion】 The indica rice varieties with national high-quality had advantages of high head rice, narrow grain shape, little white-belly, good transparency, moderate amylose content and long gel consistency. The difference of protein content was not obvious among these indica rice varieties with national high-quality, but the difference of fatty acid content was obvious. The rice varieties of Yuexiuzhan, Bataixiang1 and Yuejingsimiao2 had higher fatty acid contents. In general, the RVA profiles of indica rice varieties with national high-quality had higher breakdown and lower setback values, and their eating qualities were better than those of indica rice varieties with non-national quality. The breakdown and setback values of Yuejingsimiao2，which was an indica rice variety with good eating quality， met the recognized indicators of good eating quality. The amylose content and gel consistency were positively correlated with cool paste viscosity, breakdown and setback values. A RVA profile analysis, particularly combined the selecting of amylose content and gel consistency with the analysis of breakdown and setback values, is helpful to improve the accuracy and efficiency in rice quality breeding.