Objective To determine the control effect of sorghum vinasse against broomrape (Orbanche cumana) and the influence of vinasse on growth and quality of tobacco.Method A field plot assay was conducted and different dosages of vinases were applied in soil. The control effect of vinasse against broomrape and influence on growth, yield and quality of tobacco were investigated.Result Vinasse had good control effects against broomrape at different dosages. The average plant control effect and average fresh weight control effect of vinasse against broom rape were 62.73%−74.16% and 42.70%−66.03% respectively in 2018, and 57.10%−64.17% and 53.23%−80.56% respectively in 2019. Tobacco grew well with the application of different dosages of vinasse, and the growth indicators were significantly higher than those of the control. Tobacco yield increased significantly after applying vinasse, fresh weights increased by 17.76%−24.22% and 6.63%−15.71% than those of the control, and dry weight increased by 6.70%−11.63% and 15.39%−20.17% in 2018 and 2019, respectively. The proportion of superior and middle class of tobacco increased by 7.35% and 3.90% than those of control, in 2018 and 2019 respectively.Conclusion Sorghum vinasse can be used for the prevention and control of tobacco broomrape. It is suggested that vinasse mixed with soil in a depth of 20 cm at the dose of 1−2 kg/m² before tobacco seedling transplanting.