To protect and utilize natural enemies reasonably, spatial relationships between natural enemies and Empoasca vitis, Dialeurodes citri, Scirtothrips dorsalis and Toxopetera aurantii were investigated respectively in tea plantations in Qianshan, Anhui. The ranges of spatial dependence (RSDs) of these pests and their natural enemies were calculated by GIS and the spatial relationships among natural enemies and four pests were analyzed using grey relational analysis. The results showed that two main natural enemies (Theridion octomaculatum and Clubiona reichlini) had a close spatial following relationship with Empoasca vitis. The synthetic results demonstrated that two natural enemies (Theridion octomaculatum and Erigonidium graminicolum) had a close sptial relationship with Dialeurodes citri, while two main natural enemies of Scirtothrips dorsalis were Erigonidium graminicolum and Theridion octomaculatum in spatial relationships. And the two close natural enemies of Toxopetera aurantii were Harmonia axyridis and Erigonidium graminicolum in space.