By means of chromosome Giemsa-C banding and polyacrylamide gel electrophoresis of pcroxidase isozymes, four clones derived from Saccharum arundinaceum Retz. and their parents were analysed to identify the hybrids. The main results were as follow,1 . Among 60 chromosomes of S.arundinaceum,56 were characterized by the present of telomerlc bands, while only 2 or 4 of such chromosomes were detected in one cell of the sugarcane varieties. The differences of C-banding pattern between the male and female parents were significant.2. Clones Ya Cheng 73/07 and Ya Cheng 75/283 were true hybrids as they showed intermediate characters between the two parents both in chromosome C-banding pattern and peroxidase zymogrom. The other two, Ya Cheng 57/25 and Ya Cheng 73/512 were obviously closed to their female parents respectively.The value and behaviour of S. arundinaceum in sugarcane breeding was estimated onthe basis of chromosome C-banding patterns, and the advantage and limit of the threemethods used in identificating of hybrids were discussed.