Two virus-free garlic cultivar bulbs were obtained after culturing 0.100 -0.125mm long shoot apices on the basal medium of Murashige and Skoog(1962) supplemented with different combinations and different concentration of IAA, 2,4-D, KT. NAA and BA. The effects of these growth regulators and the influences of light and temperature to the callus growth, organogenesis and bulb formtion had been compared and discussed, a set of culturing conditions and media with growth regulators were recommended.