In the present experiment, the correlations between the changes of isozymes and fertility under different natural conditions, different day-light lengths and different temperature treatments were studied by means of the pollen fertility index, using the photoperiod-temperature sensitive genie male-sterile lines (NongKen58S, Shuang8-2S, N9RS). At the same time, the linkage relationships between isozyme genes and sterility genes were tested. The results indicated that there were close relationships between Adh-1, Est-3 and sterility. In the case of fertility and sterility, both of the loci expressed null alleles and active alleles respectiv -ely. One of sterility genes Jinked to Adh-1 with a recombination value of 0, another gene linked to Cat-1 with a recombination value of 29 percent.