Calli were induced from germinating seeds of indica rice ( Orgza saliva L. indica variety )" Qiuguiai 11" and the embryogenetic calli were selected and transferred to N6 liquid media in which embryogenetic cell suspension cultures were established after 6 months' subculture. Protoplasts were isolated from suspension cultures and imbedded in KpR agarose media. Without using nurse culture, the division frequency was 11.36%. The regenerated cell colonies were transferred onto N6 differentiation medium in which green plant formed. The plant regeneration frequency was 9. 4%.