Three kinds of extracting methods and six kinds of solvents were applied to extract the leaves of Tephrosia vogelii Hook f.. the content of rotenone in the extracts and dry powder of leaves were detected by HPLC. It showed that the content of rotenone in the crude extracts extracted by chloroform with vibrating extraction from purple variety was the highest to 9.70% , and the respond content of rotenone in dry powder of leaves of T. vogelii extracted by acetone with Sohxlet from purple variety was the highest to 0.72%. Moderate polarity solvents such as chloroform, benzene, acetone and ethyl acetate were proper to extract rotenone, while chloroform or acetone used in laboratory and benzene used in factory were appropriate.