Hormone contents in young spikes were determined by enzyme-linked immunosorbent assay and yield characters were investigated in Huahang No.1, Huahang No.8 and their original cultivars. There are no significant differences in the ratio of indole acetic acid to isopentenyladenine [b(IAA) /b(iPAs)] in the young spike, as well as grains per spike and the seed setting percentage between Huahang No.1 and its original cultivar Texianzhan 13. The ratio of b(IAA) /b(iPAs) in the young spike of Huahang No.8 is significantly lower than that in its orginal cultivar Yuexiangzhan. The grains per spike of Huahang No.8 is significantly higher than that of Yuexiangzhan. The seed setting percentage of Huahang No.8 is significantly lower than that of Yuexiangzhan. The ratio of b(IAA) /b(iPAs) in young spikes is negatively correlated to grains per spike, and positively correlated to seed setting percentage.