The effect of high-voltage electrostatic field on the germinating energy, dehydrogenase and POD activities, soluble protein content and super-weak luminescence for Chinese cabbage and cucumber imbibed seeds treated with different dosages was looked at. The test results indicated that high-voltage electrostatic field had obvious effect in improving seed vigor and seed germination, and the super-weak luminescence of seeds treated with high-voltage electrostatic field became significantly stronger. The changes in super-weak luminescence, physiological and biochemical indices showed significant correlation, indicating that super-weak luminescence could be an accurate, fast and sensitive physical means in choosing the optimal electric field treatment dose for seeds.