Agrobacterium-mediated transformation of pepper 'B162'with coat protein gene of cucumber mosaic virus(CMV-CP) was studied.Results showed that the best transformation system was: cotyledons explants were precultured for 2 d,and then inoculated with Agrobacterium tumefaciens(D600 nm= 0.3) for 7 min,transferred onto selection differentiation medium after co-cultured for 2 d.10 green adventitious buds with Kan-resistance were screened by Polymerase chain reaction.Two of them were amplified with the band of the fragment of 740 bp.The result showed the CMV-CP gene had been incorporated into the plant genome successfully.