The pot experiment and soil incubation experiment were conducted to study the effect of bio-organic fertilizer on Brassica alboglabra, Chinese kale, growth and soil N, P, K content. The results showed that bio-organic fertilizer could significantly increase yield of the Chinese kale particularly in low-fertility soil. Application of bio-organic fertilizer could improve the accumulation of N, P, K in Chinese kale. The nutrient accumulation was increased by the increase of fertilizer application. Bio-organic fertilizer remarkably improved the soil supplying fertilizer ability, and raised the soil N, P, K content.