To study the potential value of a recombinant pseudorabies virus TK-/gG-/ORF2+ as candidate vaccine,the biological characteristics of this virus were examined,including growth properties,genetic stability,security,immunogenicity and protective capacity.The growth properties of the recombinant virus in cells were similar to that of the parent vector virus.In animal experiments,TK-/gG-/ORF2+ was safe to mice with dose of 100LD50 and elicited specific immune response against PRV and PCV2 in mice.Immunization with TK-/gG-/ORF2+ protected mice against a lethal challenge(100LD50)with a virulent PRV Ea strain.