Series of experiments of genetic analysis were carried out in this paper.The main results obtained as follows:(1) The low amylose content trait in XLA-1 was controlled by two recessive linked genes.The two genes had complementary interaction and any homozygous gene can reduce the amylase content.In these two genes,one was possibly a multiple allele to Wx gene and another gene maybe a new mutant gene.(2) One major recessive gene and several minor genes controlled the low amylose content trait in XLA-2.This gene maybe a multiple allele to Wx gene.(3) The genomic DNA polymorphism analysis with the(CT) n microsatellite marker 484/485 of Wx gene indicated that the genomic DNA polymorphism had no difference among XLA-1,XLA-2 and Jingxiangnuo(glutinous rice),but the amylase content of XLA-1,XLA-2 were 14.42% and 11.59%,respectively.The results showed that there were other genetic factors which could affect the amylase content.The result coincided with the genetic analysis.