Detection of PRRSV Variant Strain in Boar Semen and Sequence Analysis of NSP2 and ORF5 Genes

doi:10.7671/j.issn.1001-411X.2009.02.028

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Detection of PRRSV Variant Strain in Boar Semen and Sequence Analysis of NSP2 and ORF5 Genes
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                        10.7671/j.issn.1001-411X.2009.02.028
                    
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Porcine reproductive and respiratory syndrome virus(PRRSV) variant strains(NSP2 1 594-1 680 deletion) were identified by real-time RT-PCR from boar semen of a pig farm.cDNA of NSP2 and ORF5 genes of one strain named GDTS were cloned and sequenced.The results showed that NSP2 gene was composed of 950 amino acids,which had 1 amino acid deletion at the positions of 481 and 29 amino acids deletion at the positions of 532-560 compared with that of CH-1a and VR-2332,which had the same deletion compared with that ...

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WANG Lian-xiang, SUN Yan-wei, MA Jing-yun, ZHANG Guan-qun, XIE Qing-mei, CHEN Feng, BI Ying-zuo, YU Kang-zhen. Detection of PRRSV Variant Strain in Boar Semen and Sequence Analysis of NSP2 and ORF5 Genes[J]. Journal of South China Agricultural University,2009,30(2):

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