The technique of SSR markers was used to study the genetic diversity of the sweet corn inbred lines，56 pairs of primers selected from 150 pairs of primers. A total of 155 polymorphic bands，an aver-age of 2. 77 alleles per primer，variation of 2-5 alleles for each primer , mean polymorphic information content of 0. 42 and its range from 0. 13 to 0. 71 were detected. Clustering analysis in UPGMA classified the sweet corn inbred lines into 3 groups，which were similar to germplasmic genealogy.