The influences of five different added dosages of earthworm manure on anaerobic digestion of food waste were investigated at（37±1）℃. The variations of pH, SCOD, VFAs, TP and biogas yield in the anaerobic digestion processes were studied. The experimental results showed that the accumulations of biogas yield were 14 700, 16 074, 15 702, 16 056, 16 414 and 16 485 mL and the biogas yield rate reached 466.35, 504.84, 488.02, 494.03, 499.67 and 496.69 mL·g^-1 when the earthworm manure added were 0, 3%, 6%, 9%, 12% and 15%（w, wet substrate）, respectively, and their outputs were increased by 8.25%, 4.60%, 5.93%, 7.14% and 6.51% , respectively. The earthworm manure could enhance the biogas production most significantly at the dosage of 3%. During the process of the anaerobic digestion, the addition of earthworm manure could improve the pH value and the hydrogensis and acidogenesis rate of food waste significantly. Therefore, the addition of earthworm manure could prevent VFAs accumulation, buffer the decrease of pH value and increase the biogas yield.