An intergeneric hybridization between Ajania and Chrysanthemum was produced by hand pollination in this study to introduce new germplasm into Chrysanthemum gene pool. One intergeneric hybrid of Ajania achilloides×Chrysanthemum morifolium‘Beijin’ was identified by morphological characteristics comparison and cytological analysis including flow cytometry. The hybrid was identified by the characteristic with ray florets which were quite different from that of maternal parents. It was found that the hybrid was tetraploid while the parents were diploid and hexploid based on cytological studies. The intergeneric hybrid could be used as the bridge for breeding of drought-resistant chrysanthemums.