Morphological and molecular characterization of two putative Xiphinema americanum-group species (X. brevicollum and X. himalayense) from Yaccatree imported from Japan were described. ITS and D2D3 segments of two species were amplified, cloned, sequenced and analysed by using ME polygenetic tree. They were first intercepted by the General Administration of Quality Supervision Inspection and Quarantine of the People’s Republic of China. X. himalayense was reported in Japan and has no distribution record in China.