The comparison on chemical, biological activation and combination of chemical-biological activation on potassium release from K-feldspar were performed by shake flask culture and pot experiment. The results showed that the effects of chemical and biological activation on potassium released from K-feldspar were obvious and the amount of released potassium increased. The activated potassium released from K-feldspar with chemical, biological activation could promote the growth of maize. Between the two treatments, chemical activation was better. The effects of combination of chemical and biological activation were better than that of the single activation, which showed that the synergistic effect on combination of chemical and biological activation.