The enduring fitness to low temperature of six isolates of Radopholus similis from ornamental plants was studied through longterm culture propagation on carrot callus and rejuvenation culturing on Anthurium andraeanum. The tolerance to low temperature of R. similis was determined by culturing on carrot callus. The results showed that the tolerance to low temperature of R. similis reduced after successive culturing on carrot callus for six generations, and the survival and fecundity decreased significantly. After rejuvenation on A. andraeanum, the survival and fecundity of R. similis could recover in different degrees, namely the resistant capacity to low temperature was enhanced. The tolerance to low temperature of R. similis was different in longterm cultured carrot callus according to the varying nematode groups. Similarly, the degree of recovery of the resistance to low temperature was also different after being cultured on A. andraeanum.