In this paper, the residues and depuration of semicarbazide(SEM) in hybrid snakeheads（Channa maculata(♀)×Channa argus(♂)）were tested. Hybrid snakeheads were drug-bathed 2 days with 2 mg/L nitrofurazone at temperatures of (30.5±0.5) ℃.During drug-bath, the samples were collected at 0.5,1,2,4,8,12,24,48 h,and at 0.5,1,2,4,8,12,24,48,96,192,288,384,480,720,960,1 200,1 440,1 920,2 400,2 880 h after drug-bath, and the SEM residues in muscle were measured by high performance liquid chromatography tandem mass spectrometry(HPLC-MS).The results showed that the SEM residue from 0.5 to 48 h in hybrid snakehead muscle increased gradually from （32.58±2.82） to（175.92±18.49）μg/kg during drug-bath. SEM residue was found to be the highest,（108.00 ±5.86）μg/kg at 1 h after drug-bath. The residues ranged between（53.60±4.90）and（69.21±5.71）μg/kg from 2 to 48 h after drug-bath but significantly decreased after 48 h, which was below the detection limit of 0.25 μg/kg at 1 440 h. The average degradation of SEM in muscle was 0.089 μg/（kg·h）. The results showed that the nitrofurazone of muscle in this experimental condition was degraded after 60 days.