In order to master the squeeze breaking mechanism of peanut seeds and reduce the mechanical damage, the squeeze breaking property of the peanut seeds of different varieties, under different moisture contents and different squeeze locations have been analyzed through the squeeze breaking experiments with the particle strength test instruments. The mechanical properties of peanut seeds of different varieties, under different moisture contents and different squeeze locations were analyzed. The experimental results showed that the maximum squeeze force of peanut seeds under the different moisture contents and different squeeze locations had significant differences. The maximum squeeze forces of peanut seeds were reduced ultimately with the increase of the moisture contents, and the maximum squeeze force of peanut seeds under the different squeeze locations and the same moisture contents followed in an order of the ventral side, the side surface and the top surface. At the same squeeze location, the maximum squeeze force of different varieties were different, which was related to the internal composition and dimensions of peanut seeds.