Two mangrove endophytic fungi K38 and E33 from the South China Sea Coast were chosen to be co-clutured. Nine sterols, 24-methylcholesta-5,24(28)-diene-3β,15β,19-triol（compound 1）,24-methylcholesta-5, 24(28)-diene-3β,19-diol (compound 2), 3β,11α-dihydroxyergosta-8, 24(28)-diene-7-one(compound 3),(22E, 24R)-ergosta-5, 22-diene-3β-hydroxyl-7-one (compound 4), (22E, 24R)-6β-methoxyergosta-5, 22-diene-3β, 5α-diol（compound 5）, (22E, 24R)-5α, 6α-epoxyergosta-8, 22-diene-3β,7α-diol(compound 6), ergosterol, ergosterol peroxide and (22E, 24R)-ergosta-7, 22-diene-3β, 5α, 6β-triol were isolated from the crude extracts of mycelia. Among them, compound 1 and compound 2 were found in marine mangrove fungi for the first time, and compounds 1~6 were not obtained from these two strains by previous pure fermentation.