【Objective】 In order to solve the problems existing in the production of flue-cured tobacco about low nitrogen use efficiency (NUE) and inferior quality of flue-cured tobacco by unreasonable fertilizing technology.【Method】 A variety (Yunyan87) was used as a material in the field experiment. The technology of real time nitrogen management (RTNM) was applied in Meizhou. Two application modes of fertilizer (S40 and S43) based on SPAD（Soil and Plant Analyzer Development）value, the conventional application of fertilizer by farmer (CK2) and no fertilizer (CK1) were designed to study the influences of different application modes of fertilizer on the agronomic traits, nitrogen use efficiency (NUC), yield and quality. 【Result and conclusion】 The areas of the middle and upper leaves increased by adopting RTNM, and the other agronomic traits had no significant differences compared with CK2. The NUE was elevated obviously under RTNM, and the absorption of nitrogen of S40 and S43 increased by 48.74% and 45.42% compared with CK2, respectively. The yield and output value of tobacco leaf under RTNM increased more than those of CK2 and S43 achieved the highest yield and output value of tobacco leaf. Results indicated that, compared with the conventional application of fertilizer by farmer, RTNM could enhance high yield and output value of flue-cured tobacco and improve utilization rate of nitrogen fertilizer. Therefore, RTNM, especially based 43, is effective in practice and good for popularization at Meizhou Guangdong production area according the local condition.