【Objective】To investigate the antimicrobial resistance and molecular types of Salmonella Senftenberg isolates from food of animal origins in Shanghai. 【Method】A total of 15 Salmonella Senftenberg isolates were collected from 2008 to 2012 in Shanghai.Agar dilution method and pulse field gel electrophoresis （PFGE） were applied to the determination of minimum inhibitory concentrations （MICs） and molecular typing， respectively. 【Result and conclusion】The 15 isolates exhibited resistance most often to sulfisoxazole（46.7%）and they were fully sensitive to the remaining antibiotics expect for one which was resistant to streptomycin （6.67%）. PFGE analyses of 15 Salmonella Senftenberg isolates resulted in 10 unique patterns， among which three （X3， X4， X5） were grouped together at a high similarity index of 88.2%， su-ggesting that these isolates of different origins may be relevant in the epidemiology.