【Objective】To find specific affinity peptide which inhibited replication of canine influenza virus(CIV),and the ligands on the CIV Hemagglutinin HA1 subunits were screened from the phage display random library.【Method】The CIV Hemagglutinin HA1 subunits as the target were screened out through affinity selection from the heptapeptide phage library, and the affinity peptide which possessed anti-viral properties of H3N2 subtype of influenza virus were tested in chicken embryos or vitro.【Result and conclusion】After four rounds of affinity panning, six peptides were identified. Six peptides have various degrees of an antiviral activity of H3N2 subtype in vitro or vivo, especially the peptide HA-4,which shows the strongest antiviral activity.Experimental results show that the phage random peptide library technology can be used in the antiviral research.