Rapid detection of classical swine fever virus by loop-mediatedisothermal amplification combined with lateral flow dipstick method
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    Abstract:

    【Objective】 The experiment was conducted to establish a rapid real time detection method of classical swine fever virus at local fields. 【Method】 A rapid detection method(RT-LAMP-LFD) of classical swine fever virus(CSFV) was developed by loop-mediated isothermal amplification (LAMP) combined with lateral flow dipstick (LFD) method. The LAMP method for amplification of the CSFV NS5B gene using fluorescein isothiocyanate-labeled primer was combined with a chromatographic LFD for rapid and simple visual detection of CSFV. 【Result】 This method could specifically detect CSFV, but not other virus such as PRRSV, JEV and PCV-2. The minimum detection limit of the CSFV-RT-LAMP-LFD method was 50 pg. The detection could be finished within 1 h without using complex instruments such as PCR machine and the results were visible by eye. 【Conclusion】 This RT-LAMP-LFD method is a highly specific and sensitive, safe, rapid and simple way for field detection of CSFV infection.

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ZHU Junling, YE Zuodong, DENG Jieru, GOU Hongchao, CHEN Jinding. Rapid detection of classical swine fever virus by loop-mediatedisothermal amplification combined with lateral flow dipstick method[J]. Journal of South China Agricultural University,2016,37(1):1-7

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History
  • Received:April 13,2015
  • Revised:
  • Adopted:
  • Online: January 08,2016
  • Published: