【Objective】To study whether SNPs of TRAPPC9, DGAT1, ATP1A1 and GHR genes were associated with milk production traits in Holstein dairy cow in Southern China. 【Method】The Sequenom MassARRAY IPLEX genotyping technology was applied for SNP genotyping, and SAS software was used for statistical analysis.【Result】The association analysis showed that the rs110017379 SNP of TRAPPC9 had significant effects on protein percentage(PP) and fat percentage(FP)(P<0.05)， the rs109421300 of DGAT1 had significant effects on FP (P<0.05)， the rs110256520 of ATP1A1 had significant effects on PP (P<0.05)， and the rs41639260 of GHR had significant effects on both PP and FP (P<0.05).【Conclusion】The SNPs of TRAPPC9, DGAT1, ATP1A1 and GHR genes can be used in molecular marker assisted selection of Holstein dairy cow in Southern China.