【Objective】To utilize the superior properties of mature Acacia auriculiformis, improve propagation efficiency, and accelerate breeding and promotion of elite varieties.【Method】A. auriculiformis bud inducing system was established by comparing the disinfection strategies for the explants from 16-year-old A. auriculiformis and screening the bud inducing media. The proliferation media were screened and the relationship between subculture times and proliferation rate was studied. 【Result】 The optimal explants were those collected from the 3th-5th node of shoots in August. For the optimal explants treated with 0.1% mercuric chloride for 18 min and 75% ethanol for 15 s, and inoculated onto the best bud inducing medium containing MS medium + 6-BA 1.0 mg·L^-1+sucrose 40 g·L^-1, the germination rate reached 92.00%. When type II medium (MS+ 6-BA 1.0 mg·L^-1+ NAA 0.1 mg·L^-1+Ac 0.05 g·L^-1+sucrose 30 g·L^-1) was used for proliferation, the proliferation rate was high and stable with an average rate of 2.63 for seven generations. 【Conclusion】 This tissue culture system enables fast propagation for mature elite plants of A. auriculiformis.