【Objective】 To investigate the effects of azadirachtin on cell proliferation，glucose uptake and insulin secretion in min6 cells and their possible mechanisms． 【Method】 The min6 cells were cultured in vitro and treated with various concentrations of azadirachtin for 48 h．Cell proliferation was measured. In the presence of 5.5 and 25.0 mmol·L^-1 glucose，glucose uptake was measured and insulin secretion in min6 cells were detected by the insulin ELISA kit. 【Result】 Azadirachtin promoted the proliferation of min6 cells in the presence of 5.5 and 25.0 mmol·L^-1 glucose compared to the control group. Compared with the 25.0 mmol·L^-1 glucose condition，azadirachtin in the presence of 5.5 mmol·L^-1 glucose more significantly promoted glucose uptake and insulin secretion in min6 cells. 【Conclusion】Azadirachtin could affect insulin secretion by regulating glucose concentration.