【Objective】To improve the anti-mold property of heat-treated wood and provide a technical support for expanding the use range of heat-treated wood.【Method】The Pinus massoniana wood was dipped in copper impregnated liquid with pressure according to liquid reduction reaction principle. The heat-treated wood was obtained by heat treatment technics, and anti-mold property of the wood was tested by modified mold culture box test method.【Result】The control group of P. massoniana wood was treated more than 3 hours at 220 ℃ and the mold-resistance effectiveness was lower than 20%. Under the same heat treatment condition, the mold-resistance effectiveness of P. massoniana wood which was impregnated in copper solution was more than 90%. The data of scanning electron microscope (SEM), energy disperse spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) showed that nano-copper was generated in copper-contained P. massoniana heat-treated wood.【Conclusion】The 100 nm copper produced in situ by heat treatment of copper-contained wood can greatly improve anti-mold property of wood.