Objective To study biophysical property, loading morphology and loading mechanism of nanocarrier-gene complex prepared by magnetic nanoparticle (MNP) loading plasmind DNA, and lay a foundation for application of oriented editing technology based on magnetic nano carrier.Method MNP was used as gene carrier to prepare nanocarrier-gene complex MNP-pRGEB32 with plasmid DNA pRGEB32. The abilities of MNP loading and protecting pRGEB32 were analyzed. The particle size distribution, Zeta potential and loading morphology of MNP-pRGEB32 were investigated.Result MNP compressed, adsorbed and aggregated pRGEB32 through electrostatic interaction to form nanocarrier-gene complex. MNP could effectively load and protect pRGEB32.Conclusion MNP can be used as an ideal gene transfer carrier.