Objective To explore the role of Arabidopsis H3K27 methyltransferase CURLY LEAF (CLF) in temperature morphogenesis. Method The differentially expressed genes were screened by phenotypic analysis and transcriptome analysis of Arabidopsis wild type Col-0 and mutant clf-29 under different temperatures of 22 and 16 ℃.Result clf-29 showed significant phenotypic differences under different temperatures, there was less difference between clf-29 and Col-0 at 16 ℃ than at 22 ℃. Transcriptome analysis found that deletion of CLF led to expression changes of a large number of genes, which were divided into four types (significantly up-regulated/down-regulated only in Col-0, significantly up-regulated/down-regulated only in clf-29 mutant), containing 96 temperature responsive genes. Conclusion Arabidopsis epigenetic regulator CLF responds to ambient temperature and is involved in temperature morphogenesis.