Wu Xianhua Zhang Xiquan Wei Caifan Yang GuanfuQiu Ling Li Hanqiao
1993(1):1-9.
Abstract:Some blood protein (enzyme) polymorphisms in domestic fowls were determined by means of gel electrophoresis and some of them such as alkline phosphatase (Akp-1 and Akp-2). amylase (Amy-1 and Amy-2, esterase (Es-1), hemoglobin (Hb-1) and transferrin (Tf) were thought to be as biochemical genetic markers in the study. The origins and diversities of and relationships between 3 renowned native chicken breeds in Guangdong province, China,line diversities with in Yuehuang chicken and relationships between families of Line 102 of Yushuang chicken were studied with the above biochemical genetic markers. Amylase (Amy- 1 ) polymorphism and its relationships to the vitalities in fowls were also studied with special interests. The results showed that applying biochemical genetic markers to forming poultry lines as assistances,either applying several biochemical genetic markers was possible together or applying a certain biochemical genetic marker (e. g. Amy-1).
1993(1):10-15.
Abstract:This study was dealt with the effects of glucose and mouse serum and EDTA on Kuming mouse 1-cell embryo development in vitro. The results show that the precentage of morula embryos cultured in glucose free HECM-1 to 40. 05%, and the control only 8.14%, cultured in CZB containing 0.11mM EDTA is 61. 18%, the control 47. 44%. In conclusion, the presence of glucose in culture medium is detrimental to Kuming mouse 1-cell embryo development. EDTA supports mouse embryo developement in vitro in glucose -free medium also. Glucose lacked or not, mouse serum doesn't affected in vitro mouse embrvo development.
1993(1):16-23.
Abstract:Greenhouse experiments were conducted to determine the effects of straw, CaCO3, and MnO2 on the electrochemical and chemical kinetics and rice growth and yield in a flooded acid sulfate soil.Straw (0. 25% by weigh of the dry soil) application had a pronounced effect on soil reduction. High concentration of watersoluble Fe2+ stunted plants at early growth (but the Fe2+ concentration gradually decreased and plants had vig-orus growth at maturity. Grain and straw yields at harvest were higher. Potassium in the plant tissue was hight where straw was added.Adding CaCO2(0. 25% by weight of the dry soil) markedly increased soil soltuation pH,decreased Eh and EC,and significantly depressd concentrations of water-soluble Fe2+ ,Mn2+ and Al3+.Calcium carbonate,alone or in combination with MnO2,depressed Fe concentration in the soil solution and signficantly increased straw and grain yields. Calcium and MnOj applications also enhanced N and P content in the straw but decreased Zn content.Adding 0. 005% MnO2(by weight of the dry soil) increased the Mn in the soil solution,depressd the Fe:Mn ratio in the plant tissue,and increased grain yield significantly over the control. Results show that straw as an inexpensive amendment for acid sulfate soils merits further study.
1993(1):24-27.
Abstract:From the results of the electrophoresis experiments with different concentrations of Na2HPO4 and NaH2AsO4, and different ratios between the two salts, it was concluded that the specific adsorption of phosphate and arsenate in laterite soil reduced significantly the Zeta-potential and TEP of the colloid, but the influence of the latter was larger than that of the former. Therefore, behaviours of the specific adsorption of phosphate and arsenate on surface charge of laterite soil were similar.
Yang Xian Guan Peicong Li Baoqing
1993(1):28-32.
Abstract:The effects of four NK treatments on growth, tuber yield and quality, and uptake of nitrogen, phosphorus and potassium in potato (Solaxmn tuberosum L. cv. Gloden-crown) were studies. The results showed that high nitrogen treatments inhibited plant growth and decreased tuber yield as compared with low nitrogen treatments when potassium treatments were the same. Increased potassium fertilizers enhanced markedly plant growth and increased tuber yield in low nitrogen treatments, while inhibited plant growth and decreased tuber yield in high nitrogen treatments. Low N+ high K treatment gave best yield, quality and commodity rate of tuber among treatments. NK interaction caused marked effects on content of nitrogen and potassium, while no on that of phosphorus in plants. The correlation coefficient between nitrogen or phosphorus content in plants and tuber yield was not over the level of negative significance, and that significant corrlativity existed between potassium content in plants and tuber yield, as well as between N/K ratio and tuber yield, the correlation coefficients were 0. 9670 and -0. 9649, respectively.
Chen Yingqu Zhang Chenglin Zhang Qilun Chen Huojun
1993(1):33-38.
Abstract:The effects of N, P, and K. with respect to their rate and ratios, on the yield and quality of sweet corn were studied by quadratic regressive orthogonal combinatorial design. The results showed that the yield of commercial fresh ear could be significantly increased by applying N, P and K fertilizers in the order of N>K>P. Under the experimental condition, maximum yield (9. 6 t/ha)was obtained by a combination of 273. 0 kg N/ha, 94. 5 kg P2O3/ ha and 270. 0 kg K2O/ha, and the water-soluble sugar content of frest seeds was markedly increased with increasing K. The minimum content of water-soluble sugar was found in fresh seeds as the combination of 100. 5 kg N/ha arid 75. 0 kg P2O5/ha. Below this level, the sugar content was decreased with the increase of N and P; over this level, the sugar content was increased with the increase of N and P. With regard to considering the yield of fresh ears and the sugar content of fresh seeds, a suitable ratio of N, P and K was recommended as 273. Okg N/ha. 94. 5kg P2O3/ha and 270. Okg K,O/ha, as N, P2O3, K2O= 1 : 0. 35 : 1.
1993(1):46-51.
Abstract:The present paper is a report oi eight new plants of Bambusoideae Xrom Guangdong which are Bambusa scabriculma; B. varioaurita; Sinobambusa glabrata; Acidosasa denigrata; Oligostachyum orthotropoides; Pleiobiastus hispidulus; Arundinaria bicorniculata ; Pseudosasa amplexicaulis.
1993(1):52-59.
Abstract:The present paper is a partial revision of Asian, American and Afnican Apooyuales. It treats Apocynccene and Asclepiadacece, Eighteen genera and thirty-seven species, including fifteen mew names and one new combination: Peripoca aesseiaua, P. mexicoeusis, Tylophora cordigera, Cywutchum mvrilloi, C. belloi ,C.stelligerum, C. mexicoense. C. paralius,C. fimbricoronum, C.nonellum, C.rotifvrme, Gonolobus krutiensis, G. ecuadorensis. Also, seventeen names reduced to synonymy: Kopsia lancibracteolata=X. arburea, Anvdendron kowii=A. oblongifolium, Xylinoboriopsis napecnsis=Bcdysanthera micraotha, Melolixus amamensis= M. suaveolens, Rauvolfia cmnbodiona=R.verticillata, Alyxia blenune=A.sicnnensis, Tylophora atrofolliculata and T. panzkutenga = T. Hirsuta. T. renckongii = T. globra, T. longipedicellata = T. pauciflora, T. trichophylla = T. rotundifolia, Hoya haincnensis = H. ovalifolia, H. formosana = Helerostemma brownii, Dischilia esquirolii = D. toukiuensis,D. mnnrnuleria var. rhombifolia = D. rhombifolia, Cynanchum parviflorum = C.parolies, and eleven species are new distributions.
1993(1):60-64.
Abstract:Studies were carried on the mechanism for drought resistance, using stable callus resistant to physiological drought as material. The results showed: The content of dry matter was obviously higher than that of CK. Its water potential was - 10. 76bar, while the water potential of CK was -4. 77bar. Proline content was 4. 4 times as high as that of CK,and the content of k as well as sucrose was slightly lower than that of CK The content of Abscisic Acid was 3. 4 times as high as that of CK.but obviously lower than that of CK stressed on medium containing 20% PEG for four days. New proteins or polypeptides (M. W 17000. 18000 dorton) were produced in the calllus. The property of cell membrane was improved. An assumption of the mechanism for drought resistance was put forward.
1993(1):68-75.
Abstract:The frequent application of pesticides against Citrus pests and diseases such as leaf-miner (Phyllocuistis citrclla Stainten) . red mite (Pauxychus citri), rust mite (Phyllocotruta vlcivora) and cankes (Xanthomonas citri) has resulted in the resistance to pesticides, environmental pollution, death of the natural enemies and break up of ecological balances. The present study is based on the theory of ecology and the knowledge of the developmental patterns of the pests and diseases. A series of integrated techniques of prevention and control, consisted mainly of advanced cultural practices including removal or cut-back of the summer shoot, stimulation of the autumn flush at planned dates and removal of the diseased leaves before the growing-out of each flush in order to reduce the ground population of pests and the amount of disease organisms, were proposed in combination with the improvement of orchard ecological environment including use of proper weed cover and establishment of windbreaks in the orchards in order to stimulate the multiplication of the natural enemies and to prevent Citrus psylle (Diaphorina citri) dispersion and spread, development of a convenient forecasting method, and determination of population indices and adoption of spring orchard cleaning in stead of winter orchard cleaning for effective spraying. After experimentation at 6 locations with at least 20, 000 hactares of citrus groves from 1978 to 1989, we have gained significant ecological, economical and social benefits. Especially the spraying expenses were reduced by half.
Jiang Zhiqiang Wu Jung-Tsung Zhang Liangyou
1993(1):76-83.
Abstract:The present study was designed to obtain detail information on the relationship between the populations of different virulence and biotype formation oX BPH led on IR26 variety with Bphl resistant gene. It seemed that a distinct difference of individual virulence existed in the laboratory population,especially in resistant varieties IR26 and ASD7. The results showed that the different populations, of which the virulence was determined by the amount of honeydew excreted on IR26, had no significant difference in the degree of their adaptation to TR26 under the selection pressure. Using the seedling bulk test and the population build-up experiment, the 3 selected insect populations reared on IR26 in 10th generation were able to kill IR26 and adapted to these host plants but could't survive well on Mudgo variety with Bphl gene. In addition, the amount of honeydew excretion, survival and adult weight on IR26 increased during the selection process, the shift in the insect populations to a more virulent biotype was also confirmed by these tests.
Wu Hongji Wu Jungtsung Wang Li
1993(1):84-89.
Abstract:This paper deals with the resistance of rice variety to the maize weevil. Of the 53 rice varieties tested, 39 exhibited resistant and three moderately resistant. The resistance that was evaluated on the basis of no-choice screening test had the same trend as that based on free choice screening test Highly significant positive correlation (P<0. 001) existed between susceptibility index and percentage Of gaping glumes.No corrlation (P>0. 05) between protein, starch, amylopectin, amylose content, gelatinization temperature and susceptibility index was demonstrated.
Liang Bo Su Xing He Zhaoheng Li Yizhen
1993(1):90-96.
Abstract:本文旨在探讨制定松突圆蚧(Hemilerlesia pilysophila Takagi)防治指标的方法.根据虫害发生特点,从个体及群体两种水平进行了研究.个体危害程度可分为4个等级,结果表明Ⅱ至Ⅳ级不同树冠层的虫口密度呈有规律变化,虫口密度随危害程度增大而减小;Ⅲ级后材积连年生长量显著下降,Ⅳ级超过极显著水平.在群体(即林分)水平来看,稔山平均虫口密度明显高于寨场山.以林分受害指数作为群体受害程度的测度.惠东县寨场山、稔山及海丰县圆墩平均受害指数分别为37.49%、28.42%和33.95%.调查数据表明,林分材积损失率随林分受害指数变化大致为一条Logistic曲线,通过计算机拟合,得林分材积损失率的估测模型.根据防治指标定义和估测模型推导出寨场山、稔山及圆墩3种立地类型的防治指标依次为34%、47%及30%(以受害指数表示),由经济为害水平(EIL)定义出发,我们得到相应的3种防治决策函数.
1993(1):97-102.
Abstract:Insecticidal activities of the essential oil from Artcmisin scoporia are first reported in this paper. By means of bioasaay,capaillene was verilied to be insecticidal component of the oil. The pbotoactivily of capillene and its inhibitory effect on esterate are alao dixussed.
1993(1):103-108.
Abstract:从1990年开始,针对我国三大类型水稻区稗草的化学防除历史,采用四种系统的方法,测定了稗草对丁草胺的抗药性水平.结果显示:丁草胺使用年限在5年以下地区的稗草,未测得其有明显的抗药性,但使用年限在8~12年的,产生了比较明显的抗药性.抗性水平由北向南有逐渐明显增高的趋势,双季稻区高于单季稻区,单双季稻区处于两者之间.毒力抗性比值在1.9~2.9;α-淀粉酶活性比为2.3~4.1;等浓度效果降低2/3;生长量敏感性比为2.5~4.0
1993(1):109-113.
Abstract:Root rot disease of slash pine was further confirmed to be mainly caused by Diphlodia pinea (Desm. ) Kickx. It was also found that Sphaeropsis sapinea (Fr. ) Dyko & Sutton was one of the causal agents. The mentioned 5. sapinea was quite different from the usual D. piuea (synonymy: S. saqinea) in morthology, cultural characters and germination and may be a variety of this species. Inoculation tests showed that both D. pinea and S. sapiuea could make 2-year -old seedlinks as well as 16-year-old weakened uees of slash pine dead, and that the infection of the pathogens was closely related to the defoliation of slash pine caused by Dendrolinus punctatus.
1993(1):114-121.
Abstract:马尾松毛虫(D? p? Walker)质型多角体病毒(DpCPV)能交叉感染粉纹夜蛾(Trichoph?)、斜纹夜蛾(Spadop?)、亚洲玉米螟(O?)、大蜡螟(Go?riamallonella)、菜青虫(P?)等鳞翅目幼虫,致病程度随昆虫种的不同而有较大差异,米蛾(Corc?)幼虫则对DpCPV不敏感.DpCPV对粉纹夜蛾幼虫的致死率可达100%.从粉纹夜蛾幼虫中复制出来的病毒(Tn-DpCPV)对松毛虫3~4龄和4~5龄幼虫的LC_?分别为1.73×10~5和3.06×10~?.在粉纹夜蛾幼虫中肠细胞中,多角体的形成是以小块状结晶开始,边包埋病毒粒子边相互聚合而成.
Zeng Zenling Chen Zbangliu Dong Libo
1993(1):122-127.
Abstract:本研究建立了测定吡喹酮、氯霉素、喹乙醇及利血平浓度的反相高效液相色谱分析方法.以ODS-C18化学键合相(5μm)作为固定相;乙醚萃取血浆中的吡喹酮,组织喹乙醇用乙酸乙酯-乙腈(3:2,V/V)抽提,虾肉氯霉素及组织利血平均用乙酸乙酯提取;甲醇-水为流动相;吡喹酮、氯霉素、喹乙醇及利血平进行紫外检测的波长分别是211、280、260及254nm,最低检出浓度分别是0.02μg/ml血浆、0.02μg/g虾肉、0.2μg/g组织及0.1μg/g组织,方法回收率分别是98.07±3.24、90.12±4.71、71.23±5.49及92.14±5.68%(平均值±标准差).这些方法简单、灵敏、专一,已用于测定猪静注及内服吡喹酮后的血浆药物浓度,对虾饲喂“虾病宝7号”(含氯霉素)后虾肉中氯霉素的残留,鸡内服中毒剂量喹乙醇的组织浓度,以及鸡内服利血平的组织浓度.
Xu Jiaqiang Li Wei Chen Suzhen
1993(1):128-132.
Abstract:Pathological changes of "Cow non-leukosis lympboaarcomatosis were described detalledly in this paper. The description which would enrich and complement the knowledge of the diseease was emphasized on the morphologi-cai characteristic of lympboflasis and lymphocyte-like oncocytes.The term "Cow non-leukcsis lymphosarcomstosis giving a detailed description of patbomorphological characteristics of this disease was flntly nominated in this paper refrring to literatuea in our country.
Xu Fengcai Lu Shunduo Liao Yi Li Mingqi
1993(1):133-139.
Abstract:比较了用乙醇沉淀法和硫酸铵盐析法从“东一号”剑麻中提取蛋白酶的效果.用前者制得的粗酶经Sephadex G-25柱层析得两个蛋白峰,其中峰Ⅰ与酶活性重叠。峰Ⅰ再经SP—Sephadex C—50离子交换柱层析,pH.离子强度线性梯度洗脱得4个蛋白峰.其中峰4与酶活性重叠。此酶液经聚丙烯酰胺凝胶圆盘电泳.在每条胶柱上呈单一蛋白带;量4℃下2d后出现六角形结晶.结晶酶液在pH5.5-10.0.60℃范围内稳定性较好;其最适pH为7.5;最适温度为40℃;Km(酪蛋白)值为0.114%(w/v)用SDS—聚丙烯酰胺凝胶电泳(SDS—PAGE)测得其分子量66KD;用Sephadex G—100柱层析测得的分子量为69.9KD.用Ellman's试剂测得其游离巯基含量为4.3 mol—SH/10~5g蛋白质。
Huang Zhuolie Lin Shaoxiang Li Mingqi
1993(1):139-143.
Abstract:本研究结果表明,菠萝茎组织中含有0.54~1.16%的蛋白酶.在酶膏的干燥过程中,45℃烘干使蛋白酶活性下降61.32%,而真空干燥的酶活性只下降18.32%.研究结果发现,硫代硫酸钠和半胱氨酸混用可部分地保护酶活性,使酶活性比对照提高67.37%.在提取中,用氯化钠、醋酸锌、乙二胺四乙酸和抗坏血酸溶液洗涤酶复合物可使酶活性提高110.48%.用0.15~0.25%的丹宁作酶的沉淀剂是适宜的,既可保证酶活性,也可保证有较高的酶产量.
1993(1):144-148.
Abstract:In this paper standing crop, net production, heat energy and production structure in mixed forest of Piaus masacmiam and Castanopsis fisan of different ages were studied. The results showed: Standing crop, net production, heat energy of the mixed forest are respectively 47. 75%. 112. 99% and 46. 82% higher than those in the pure forest, and these of the trees of P. massoniana in the mixed forest are 6. 82%. 7. 60% and 7. 15% higher than those in the pure forest of P. massoniana. Production structure in mixed forest showed: Scale of the asimilative parts and the unasimila-tive parts of mixed forest are a bit bigger than those of the pure forest. The vertical biomass of mixed forest is distributed better than that of pure forest. So the light can be absorbed fully in the mixed forest. Generally speaking, the mixed forest will increase productivity and improve production structure.
Un Minzhi Xu Yingbao Gen Juyan
1993(1):149-156.
Abstract:The slope position, slope-shape, mather rock and landforme are leading factors of site classification on main cultivated areas of Albixia falcda in Guangdong province, which were selected by Multiple regression and Main-element analysis in this paper. According to these leading factors, the site of cultivated area was classified by gradual control. This paper also evaluates the productivity and suitability of Albxia falocia growing in different site unite of the cultivated area.
Chen Junlian Zeng Yongqiang Xu Xiuzhen
1993(1):157-161.
Abstract:By means of the blurred comprehensive decisive way B=A. R use this formula to build every factors' function. caculate their subordinate degree each other then combine the factors' formula to caculate every factors' compre-hensice value for the China fir cutover. The choice of the China fir cutover type offers the amount value which we may compare it.
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