CHEN Xiong hui , WAN Bang hui , LU Yan peng , PENG Hai feng , LIANG Ke qin , ZHAO Jing
2001, 22(1):1-4. DOI: 10.7671/j.issn.1001-411X.2001.01.002
Abstract:异交结实潜力是指不育系在保证有充足外源花粉供应的条件下的最高结实率、本研究表明,不同的水稻光温敏核不育系具有不同的异交结实潜力。异交结实潜力的高低受遗传、环境及遗传与环境互作的共同影响;环境对不育系异交结实潜力影响的主因是温度,其作用的敏感时期是异交籽粒灌浆充实的前期;不育系的异交结实潜力也是决定制种产量的重要因素之一。
2001, 22(1):5-8. DOI: 10.7671/j.issn.1001-411X.2001.01.003
Abstract:The morphological and anatomical characters of leaf at seedling stage of 8 sugarcane genotypes were studied. The results showed that the correlation coefficients (0.61 and 0.72) among leaf width, leaf length and vein's number were positive in very significant level. There was an extremely significant negative correlation coefficient (-0.85) between leaf width and average vein width (AVW). The correlation among the first three characters mentioned above and cane juice sugar was negative. The cane yield was negatively correlated with leaf width and vein number and positively correlated with leaf length and AVW, respectively. The path analysis showed that the leaf length directly contributed 0 559 4 value to the cane juice sugar and 0.577 1 value to the cane yield, and the leaf width directly contributed 0.475 1 value to the cane juice sugar and -0.247 5 value to the cane yield. The vein's number had negative contributions to the both cane yield and juice sugar. The results showed that the characters of seedlings, which were more narrow leaf, lower vein's number, can be used as indirect selection indices for higher yield and higher sugar content of cane.
2001, 22(1):9-12. DOI: 10.7671/j.issn.1001-411X.2001.01.004
Abstract:The paper approached the difference of the number of selected superior family between the methods of performance level analysis and simple ttest, under the condition of missing plots, missing trees and variance heterogeneity. The accuracy of simple ttest was analyzed. It was put forward that family selection can be carried out by combining performance level analysis and simple ttest. Eleven superior families were selected from the 32 tested families and the real gain for volume was estimated to be 35.8%.
ZHONG Weihua , SHI Bin , ZHOU Da , HUANG Yongquan , ZHANG Minxing , LI Yuanqiu
2001, 22(1):12-17. DOI: 10.7671/j.issn.1001-411X.2001.01.005
Abstract:对火炬松原产地耐寒与一般两类基因资源林的生长表现研究结果表明 ,前者保存率高 ,适应性强 ,生长均匀 ,差异不显著 ;后者则相反 ,保存率较低 ,生长优劣悬殊 ,差异显著 ,优于我国早年引入的种批 ,为重要的遗传改良资源 ,其中有 10个编号较优良 ,它们多产自美国东南沿海 ,尤其是墨西哥湾各州 在 31.3%入选率下 ,材积相对遗传增益大 2 0 .84% ;2~ 5株小样求得的生长早 晚相关趋势与前人研究一致 ,唯确定程度略低
LI Zhenkui , HUANG Huining , FENG Zhijian , WU Yongbin
2001, 22(1):18-22. DOI: 10.7671/j.issn.1001-411X.2001.01.006
Abstract:This article reports the investigation findings on the wild fruit plants in Nanling National Nature Reserve(NLNR), Guangdong Province. In NLNR, there are 157 species of wild fruit plants which belong to 33 families and 61 genera. Based on the characters of these resources, some suggestions about how to protect and rationally exploit them are put forward.
2001, 22(1):23-26. DOI: 10.7671/j.issn.1001-411X.2001.01.007
Abstract:研究了日本中部的3个风景林-常绿针叶林、常绿阔叶林和常绿落叶阔叶混交林的凋落物,养分归还量和养分利用效率。常绿针叶林、常绿阔叶林和常绿落叶阔叶混交林的年凋落量分别为7562、6023和5565kg/hm^2,养分年归还量分别为233.87、125.20t 140.23kg/hm^2,各养分中N、K和Ca的归还量大于P和Mg叶的养分归还量占养分年归还量的64.19%-79.29%。常绿针叶林和常绿阔叶林中 Mg的利用效率高,表明Mg可能是这2种风景林生长的限制因子。
QIU Jindan , WU Dingyao , ZHANG Hailan
2001, 22(1):27-30. DOI: 10.7671/j.issn.1001-411X.2001.01.008
Abstract:在深圳市,“石硖”龙眼(Dimocarpus longana Lour.)“梢状芽”的生理分化期在12月中旬至翌年1月,花芽形态分化在1月下旬开始,2月上旬整个“梢状芽”进入花序发育期,2月底进入经分化期,“梢状芽”的发育具有可塑性,顶端与腋生分生组织的形态分化具独立性是发生“冲梢”的解剖学基础,“冲梢”时摘小叶能促进侧花序的抽生,芽内较高水平的iPA,ABA含量和b(iPA)/b(GA1/3)比值,双及叶片较高水平的总糖含量和b(C)/b(N)比值与花诱导有关,同时较高水平的b(iPA)/b(GA1/3),ABA和b(C)/b(N)与花序原基形成有关。
2001, 22(1):30. DOI: 10.7671/j.issn.1001-411X.2001.01.009
Abstract:在第三届广东省优秀期刊评选活动中,《华南农业大学学报》荣获优秀期刊奖(科技类).在本次评选活动中,共有88种社科类期刊、72种科技类期刊参加评选.经民意推荐、分组初评或专家评审计分、省评选委员会复审定评,评出第三届广东省优秀期刊43种,其中社科类期刊26种.科技类期刊17种.
2001, 22(1):31-34. DOI: 10.7671/j.issn.1001-411X.2001.01.010
Abstract:Abscisic acid(ABA) in the peel was significantly increased after removing kraft paper bags which decreased the synthesis of phenolic substance with the result that synthesis of phenolic substance and fruit coloration were accelerated. The content of IAA and iPAs increased, and finally exceeded that of the control fruit, while the content of GA 1/3 was relatively low. The changes in endogenous hormones and phenolic substance after removal of adhesivebonded fabric bag treatment which improved coloration was compared to levels during the kraft paper bag treatment. ABA was the key endogenous hormone which adjusted anthocyanin synthesis, while iPAs and GA 1/3 also took part in anthocyanin synthesis. The forming of pigments was the combined result of several endogenous hormones.
WU Zhenxian , SU Meixia , CHEN Weixin , JI Zuoliang , LI Chubin , LIANG Xiufang
2001, 22(1):35-38. DOI: 10.7671/j.issn.1001-411X.2001.01.011
Abstract:The storage effect and physiological changes of Huaizhi, Nuomici and Guiwei cultivars with different postharvest treatments and stored under room temperature were studied. The results showed that the storage properties of litchi cv. Guiwei was better than cvs. Nuomici and Huaizhi. Proper postharvest treatments could extend storage life. Packaging with polyethylene boxes was better than polyethylene bags in the beginning 4 d; storage in the airconditioned room could also extend storage life. Determination of fruit respiration, nutrition and the polyphenol oxidase and peroxidase activities indicated that they were related to the fruit storage properties.
2001, 22(1):39-41,45. DOI: 10.7671/j.issn.1001-411X.2001.01.012
Abstract:Influence of two kinds of selection agents on the tissue culture of ten genotypes with different resistance to fusarium wilt in cucumber were studied .The results showed that the suitable agents of in vitro evaluation were the culture filtrates of Fusarium oxysporum f.sp. cucumerinum(FOC)and 1 mg/L crude fusaric acid (FA )extracts ,and the suitable period for identification was the induction of callus phase .The tissue culture resistance to agents showed consistence with the seedling resistance to the pathogen .
2001, 22(1):42-45. DOI: 10.7671/j.issn.1001-411X.2001.01.013
Abstract:In this paper, the rotational composite design of quadratic regression was used to study the effects and interactions of four factors (the number of parasitoids released ,the density of host eggs, the size of searching patch and the size of the crops) on parasitism of Trichogramma pretiosum to the eggs of the diamondback moth(DBM), Plutella xylostella in the field. It was showed that the number of parasitoids released , the size of the crops and the size of searching patch influenced parasitism significantly in turn. The density of host eggs had only minor effect on parasitism. Interaction between the number of parasitoids released and the size of the crops was the most dominant factor among interaction of different factors affecting parasitism of T. pretiosum. T. pretiosum had a strong searching capacity to the egg of DBM, but there existed interference between individuals. Based on the data a quadratic regression parasitism model of T. pretiosum to the eggs of DBM was constructed.
TANG Cai , HUANG Dechao , TONG Xiaoli , HUANG Zhi
2001, 22(1):46-48. DOI: 10.7671/j.issn.1001-411X.2001.01.014
Abstract:研究了9个梯度变温对湿地松粉蚧Oracella acuta(Lobdell)越冬代成虫和第一代卵的影响,结果表明:湿地松粉蚧雌成虫存活率随温度升高逐渐下降,高温对成虫产卵有一定抑制作用,持续较高温度处理成虫,其产卵量显著降低;湿地松粉蚧成虫生长发育的最适温度为27-21℃,卵的孵化和发育的最适变温组合是29-23℃;在29-23℃以上的温度波动对卵的发育速率有增速作用,而在29-23℃以下的温度波地卵的发育速率则有减速作用。
2001, 22(1):49-50. DOI: 10.7671/j.issn.1001-411X.2001.01.015
Abstract:The present paper describes the larvae of Afidentula manderstjernae (Mulsant) and Afissula bisquadripunctata (Gyllenhal) collected in China. The specimens are deposited in the Insects Collection of Nanjing Agricultural University.
2001, 22(1):51-53. DOI: 10.7671/j.issn.1001-411X.2001.01.016
Abstract:Five new fungal diseases on Araceae in China were identificated by systematic investigation carried out from 1997 to 1999 in Guangzhou region. The pathogens are Sclerotium rolfsii Sacc., Ascochyta philodendri Batista, Metarrhizium anisopliae (Metsch.) Sorokin, Acremonium zonatum (Saw.) W.Gams and Pythium sp..
WANG Kuitang , XU Hanhong , WEN Yanlian , ZHANG Shangqi , FU Shibo
2001, 22(1):54-55. DOI: 10.7671/j.issn.1001-411X.2001.01.017
Abstract:A reformed synthesis route of organotin miticides O,Odialkyldithiophosphosatotricyclohexyltin was reported. Tricyclohexytin hydroxide was used as starting material, and it reacted with O,O-dialkylphosphorodithioic acids to produce objective compounds. They are soluble in most organic solvents and can be processed into emulsifiable concentrate formulation.
JIAN Yuyu , CHEN Yuanling , LI Jing , GAO Lili , DONG Chun
2001, 22(1):56-59. DOI: 10.7671/j.issn.1001-411X.2001.01.018
Abstract:将天蚕抗菌肽B基因应用基因枪法导入水稻TN1发芽肿胚,获得转基因植株2株,转基因水稻T1-T5 5个世代不同株系,对bar基因、抗菌肽基因的遗传和表达进行初步研究。结果表明:转基因后代株系bar^R:bar^S在部分符合3:1分离比率。T1、T4转基因株系PCR、Southern印迹分析表明,T18株有抗菌肽B基因;T4仍有4个株系有抗菌肽基因,其他的株系基因丢失,T3 1个株系Northern印迹证明抗菌肽基因在RNA水平有表达;抗菌肽B转基因水委对白叶枯病的抗病性有提高,抗性能传递到高世代,在T3得到抗病性提高的株系。
FU Xuelin , HE Ping , ZHANG Zhisheng , ZHANG Leqing
2001, 22(1):60-62. DOI: 10.7671/j.issn.1001-411X.2001.01.019
Abstract:禾谷镰刀菌培养滤液对小麦萌发代谢影响的研究结果表明,该菌滤液毒素显著影响了小麦种子萌发过程芽和奶器官中的可溶性蛋白质、核酸、总糖等主要物质的合成积累代谢;根受到的影响较芽大;萌发种子胚乳α-淀粉酶活性也受到抑制,揭示了禾谷镰刀菌毒素影响小麦种子萌发过程中物质合成代高考 芽根器官建成的生理原因。
2001, 22(1):63-65,73. DOI: 10.7671/j.issn.1001-411X.2001.01.020
Abstract:研究结果表明,二氯苯醚菊酯(PER)对黄瓜几种氯化酶活性有不同程度的影响,其影响的程度与PER的浓度有关。20-40mg.L^-1的PER分别使黄瓜体内的过氧化氢酶和乙醇酸氧化酶活性升高3.35%-32.71%和4.42%-34.51%,20-20mg.L^-1的PER使过氧化物酶活性升高9.70%-29.10%;50-250mg.L^-1的PER使吲哚乙酸氧化酶活性升高13.58%-32.10%;50-300mg.L^-1的PER使抗坏血酸氧化酶活性下降,3.18%-20.69%,用200mg.L^-1的PER处理黄瓜幼苗1-6d后,体内过氧化物酶活升高10.375-43.17%。
JIANG Qingyan , FU Weilong , GAO Shujing , WU Ronghui
2001, 22(1):66-69. DOI: 10.7671/j.issn.1001-411X.2001.01.021
Abstract:对鸡肝细胞离体培养中几种分离肝细胞的方法进行了比较,试验选用1-56日龄的粤黄鸡,分别采用肝组织块培养、肝组织直接通过细胞筛过滤、2.5g/L胰蛋白酶活消化、2.5g/L胰蛋白酶加0.1g/L EDTA消化、不同时间的4℃冷消化与37℃消化、淋巴细胞分离液分离、5g/L氯化铵除去红细胞等试验方法,观察了鸡离体肝细胞的分离及培养效果,结果表明:采用2.5g/L胰蛋白酶加0.1g/L EDTA 消化10min,用淋巴细胞分离液分离后的肝细胞培养效果最好,肝细胞在培养液中分裂增殖,生长良好。
WANG Chong , ZHANG Shouquan , YANG Guanfu
2001, 22(1):70-73. DOI: 10.7671/j.issn.1001-411X.2001.01.022
Abstract:Inner and outer primer pair were designed and synthesized according to the cDNA sequence of liver type phosphofructokinase (PFK) of the mouse. A nested reverse transcriptasepolymerase chain reaction (Nested RTPCR) was used to determine the transcript pattern of mRNA encoding mouse PFK throughout 1,2,4,8cell and morulae embryos. The results showed that 1cell to morulae mouse embryos mRNA could be amplified to give an expected band using the inner primer pair on the templates provided by the products of the first PCR using the outer primer pair. These observations indicated that the transcripts of PFK gene existed and that the PFK of early mouse embryos is of the liver type. Glycolysis and tricarboxylic acid cycle (TCA) appear to be the important mode of glucose metabolism in 1cell to morulae mouse embryos.
2001, 22(1):74-77. DOI: 10.7671/j.issn.1001-411X.2001.01.023
Abstract:针对禽传染性支气管炎病毒(IBV)血清型众多、临床检测不易的难题,用我国自已研制的2株IBV单克隆体,通过多种方法的比较与鉴定,建立起活用于定性检测IBV的包被鼠源IBV单抗的Mab-ELISA方法,其操作程序是:包被鼠源IBV单抗-被检测病毒-鸡抗IBV高免血清-兔抗鸡IgG(AGP效价1:32)-羊抗兔IgG-HRP;用本方法对国内46个IBV分离株及NDV、AIV-H9N2、PPMV-I等的检测,表明其具有较好的特异性和敏感性。
2001, 22(1):78-80. DOI: 10.7671/j.issn.1001-411X.2001.01.024
Abstract:根据IBV Beaudette株全基因组序列,借助基因分析软件自行设计合成了3个引物(youl,you2-youM ),引物you1 和you2-在S1基因两则,跨幅为1611bp,引物you2-和youM 在S1基因的3‘端,跨幅为535bp,用引物you1 和you2-对5个IBV地方分离株(HN2、HN4、JX1、SC2和SC4)进行RT-PCR,均成功地扩增出预期大小的目的片段,对RT-PCR的产物用限制性内切酶PstI进行酶切分析,结果酶切产物中得到2条条带,大小分别为560和1050bp,与GeneBank中11株已发表的S1基因分析结果一致,初步鉴定结果显示,已经成功分离得到了IBV-S1基因。
LI Kaifu , GAO Zhenzhong , SUN Jin
2001, 22(1):81-84. DOI: 10.7671/j.issn.1001-411X.2001.01.025
Abstract:刨花板在制造和使用过程中,由于温度的变化而产生热应力,从研究结果可知,温度每增1℃,界面热应力约提高0.026MPa,界面残余应力约提高0.006MPa,而对应的界面结构强度约降低0.46MPa,这是不容忽视的影响。如何定性和定量评价热应力,是降低热应力和提高界面结合稳定性的关键。
YU Xiaolin , XU Buqian , HU Zhuoyan , WU Qing , FAN Huajin
2001, 22(1):85-87. DOI: 10.7671/j.issn.1001-411X.2001.01.026
Abstract:The chemical stability and antioxidative activity of the two kinds of watersoluble pigment material extracted from Japanese red Perilla and local Perilla were investigated. The results showed that the chemical properties of two kinds of Perillas were quite different; Pigment stability of Japanese red Perilla to heat and light was better than that of local Perilla. Both of antioxidative activity and contents of polyphenol and pigment of Japanese red Perilla are higher than that of local Perilla. There was a significant correlation between the antioxidative activity of Perillas and the level of contents of polyphenol and pigment. It was proved that there was a direct contribution of polyphenol and pigment in antioxidative activity of Perillas.
CHEN Zhangliu , YANG Guixiang , SUN Yongxue , HUANG Xianhui , ZENG Zhenling
2001, 22(1):88-91. DOI: 10.7671/j.issn.1001-411X.2001.01.027
Abstract:This paper was a review about the toxicity and ecotoxicological research of residues of veterinary drugs. Veterinary drug residues included those in the food producing animals and in the ecological environment. Residues of veterinary drugs in food producing animals may cause the harms to human consumers which include directive toxicity, effects on intestinal microflora and increase of bacterial resistance of pathogen. Ecotoxicities of veterinary drug residues in environment may manifest mainly the effect on the biological populations in the soil, effect on aquaticlife, effect on various insects and fate in the environment. The paper showed that veterinary drug residues, human health and ecological environment as well were so closely related, further studies and investigation should be carried on.
2001, 22(1):92-92. DOI: 10.7671/j.issn.1001-411X.2001.01.028
Abstract:根系的许多指标 (如根长、根质量、根吸收面积等 )与作物磷效率具有较好的相关关系 .Bonser等[1] 发现菜豆磷效率的遗传变异与根的构型有关 ,根的空间构型可能是菜豆吸收磷的一个重要影响因素 .但在大豆的研究上至今还鲜有报道 .本研究将以 2个大豆基因型为材料 ,通过特殊设计的营养袋纸培系统 ,结合计算机图象分析技术 ,定量测定大豆根构型在低磷胁迫及局部供磷条件下的适应性变化及其基因型差异 ,为通过遗传改良根构型来提高磷吸收效率提供依据 .1 材料与方法供试大豆 [Glycinemax (L .)Merrill]基因型为广州大…
2001, 22(1):93-93. DOI: 10.7671/j.issn.1001-411X.2001.01.029
Abstract:盐对植物的伤害主要是离子毒害、渗透胁迫和干扰营养物质的吸收 .植物可以通过离子泵的活动和膜的选择吸收来避免危害 ,或通过离子的调节吸收、渗透调节和渗透质的合成来增加耐盐性 .耐盐细胞系是一个较好的研究细胞水平上耐盐机制的实验系统 .本实验以小麦 81392 2耐盐细胞系为材料 ,通过比较它与原始型在不同盐浓度下溶质积累的差异来探讨溶质积累与耐盐性的关系 ,旨在为耐盐机理的研究及育种工作提供一些资料 .1 材料与方法材料的培养及处理见文 [1].脯氨酸含量测定参照张殿忠等方法[2 ] .用Z - 80 0 0日立原子吸收计测定Na+ 和K+ …
2001, 22(1):94-94. DOI: 10.7671/j.issn.1001-411X.2001.01.030
Abstract:流动和变形是聚合物加工过程中最基本的工艺特征 ,故材料的性质必然同速度、温度、压力、时间等加工参数 ,与加工过程的安排及机械设备密切相关 因此研究聚合物熔体的流变性质不但对加工出优质的制品是不可缺少的一环 ,而且对设计出符合工艺要求的设备也是十分重要的 本文研究了在引入“全程振动场”后 ,对串接于电磁动态挤出机头的圆管模头内聚合物熔体进行了数值模拟 ,在振动场中建立了以Oldroyd -Maxwell本构方程为主的动态圆管流的数学模型 ,采用了数值差分方法和移动网格法求得了数值解 ,达到对内部熔体流动的了解 ,并…
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