CHEN Fei peng , LI Mou zhao , WANG Dian bei , LAN Sheng fang
2002, 23(2):1-4. DOI: 10.7671/j.issn.1001-411X.2002.02.001
Abstract:The 18 cultivation systems in Sanshui City of Pearl River Delta was evaluated by the help of Analytic Hierarchy Process. The results showed that the four systems of rice-water chestnut, vegetable-radish-radish, wax gourd-bean-tomato and wax gourd-bean-vegetable were both good in ecological and economical benefit. The two systems of wax gourd-wax gourd-vegetable-vegetable-vegetable and wax gourd-vegetable-vegetable-vegetable were good in economical benefit, and bad in ecological benefit. The systems of rice-rice, rice-rice-potato and watermelon-rice were rather good in ecological benefit, but bad in economical benefit. There were some potentials to enhance their economical and ecological benefits in the systems of rice-peanut-sweet potato, wax gourd-wax gourd-vegetable and wax gourd-vegetable-vegetable. The composition of crops should be adjusted and the management should be improved.
2002, 23(2):5-7. DOI: 10.7671/j.issn.1001-411X.2002.02.002
Abstract:以中花芥蓝为材料,设置4个不同的温度处研究了苗期温度对芥蓝的生长发育,菜薹形成与品质的影响。结果表明,苗期温度与芥蓝的生长发育关系密切,低温可促进花芽分化,随着温度的升高,花芽分化逐渐推迟,分化时的叶位也逐渐升高,适宜的低温可促进植株生长,提高菜薹产量与品质。
WANG Wei hong , LIU Ke xing , WANG Quan yong , CHEN Zhi chuan
2002, 23(2):8-10. DOI: 10.7671/j.issn.1001-411X.2002.02.003
Abstract:The pot experiment was carried out to study fertilizer effects of two kinds of compound fertilizers that were made of Ni-removal concentrated liquor from spent electroless nickel, which the concentrations of phosphorus and nitrogen were high, on the corn and changes of the nutrients in the soil. The results showed that two concentrated liquor compound fertilizers could make the growth of corn shoots normal, and fertilizer effects were not significantly different with that of superphosphate compound fertilizer. Compared with the no fertilizer treatment, concentrated liquor compound fertilizers could remarkably increase dry mass of corn biomass and contents of N,P,K in the soil. It revealed that the concentrated-liquor compound fertilizer had obvious fertilizer effect on the corn.
2002, 23(2):11-14. DOI: 10.7671/j.issn.1001-411X.2002.02.004
Abstract:以蔗渣,木屑为原料做尾叶桉容器育苗基质,进行不同配方,堆沤及追肥处理的试验。结果表明,木屑,蔗渣经过配比,堆沤,追肥处理后可以作为尾叶桉容器育苗的基质,配方以V(木屑或者蔗渣):V(煤渣):V(黄心土)=5:2:3最好,其次为配以φ为40%煤渣,经堆沤后,基质的N、P、K含量明显上升,苗木生长指标提高,育苗性能优于或同等于泥炭的基质为:(1)V(木屑):V(煤渣):V(黄心土)=5:2:3;不堆沤用追肥2(每株施尿素0.4g KH2PO40.2g),堆沤3个月用追肥1(每株施尿素0.8g KH2PO40.4g),堆沤6个月不追肥,(2)V(木屑):V(煤渣)=6:4,V(蔗渣):V(煤渣):V(黄心土)=5:2:3,V(蔗渣):V(煤渣)=6:4;堆沤3个月用追肥1,堆沤6个月用追肥2.(3)纯蔗渣堆沤6个月,追肥2。
WU Shun hui , JIANG Cheng ai , DAI Jun , HU Yue ming , LIANG Zhong long
2002, 23(2):15-17. DOI: 10.7671/j.issn.1001-411X.2002.02.005
Abstract:Based on ArcView Internet Map Server (IMS) and World Wide Web(WWW), the WebGIS server in Soil Information System of Guangdong province was established in the paper. Long-distance clients with common WWW browser only could visit and query easily the soil spacial information of the province. It would be helpful to serve the agriculture production more widely for the soil spacial information.
SHEN Run ping , WANG Ren chao , ZHAO Xiao min
2002, 23(2):18-21. DOI: 10.7671/j.issn.1001-411X.2002.02.006
Abstract:Selection of optimal feature combinations is one of the key procedures for remotely sensed image classification and information abstraction. Based on the analysis of various quantitative methods, the differences and correlationships between them have been discussed. The method of integrated selection index for optimal feature combinations correlationships was proposed. It was proposed that the integrated selection index model constructed could be better than others.
YI Run hua , LIANG Cheng ye , ZHU Xi ru , ZHOU Er xun
2002, 23(2):22-26. DOI: 10.7671/j.issn.1001-411X.2002.02.007
Abstract:运用RAPD分子标记技术分析了从6个水稻品种上采集的40个水稻纹枯病菌菌株的遗传多样性,筛选出的10条引物共扩增出98条RAPD谱带,其中81条具有多态性,多态率为82.65%,菌株间的遗传相似性系数(以Nei‘s基因一致度表示)变幅为0.5644-0.9307,用UPGMA法可以将供试菌株分成4个RAPD聚类组群(Ⅰ、Ⅱ,Ⅲ,Ⅳ),相同寄主来源的菌株基本上聚集在同一组群内,表明同一寄主来源的菌株间具有较近的亲缘关系,在温室条件下,致病力测定结果表明所有菌株对Tetep都有致病性,病电表 指数变幅在0.40-1183之间,分析结果表明,纹枯病菌群体存在丰富的遗传多样性,不同寄主上的水稻纹枯病性,病情指数变幅在0.40-11.83之间,分析结果表明,纹枯病菌群体存在丰富的遗传多样性,不同寄主上的水稻纹枯病菌群体间发生了很大的遗传分化(FST=0.632)。这种遗传差异与寄主的选择作用有一定的关系,RAPD聚类组群的划分和菌株的寄主来源有一定的相关性,但致病力差异与菌株的寄主来源及RAPD组群没有直接相关性。
QIN Yu jie , WU Wei jian , LIANG Guang wen , ZHANG Wei qiu
2002, 23(2):27-29. DOI: 10.7671/j.issn.1001-411X.2002.02.008
Abstract:通过室内试验研究了中华微刺盲蝽 (CampylommachinensisSchuh)对节瓜蓟马若虫的捕食功能反应 ,并通过室内模拟和田间调查研究了微刺盲蝽的空间捕食效应 ,建立了捕食功能反应模型 .结果表明 :微刺盲蝽 4、5龄若虫对节瓜蓟马的功能反应属HollingⅡ型 ,微刺盲蝽成虫对节瓜蓟马若虫的功能反应属HollingⅠ型 ;盲蝽的各个虫态中 ,捕食能力大小顺序为 :成虫 >5龄若虫 >4龄若虫 >低龄若虫 .中华微刺盲蝽是我国南方控制瓜类和茄类害虫节瓜蓟马最有潜力的天敌之一
XI Ping gen , QI Pei kun , JIANG Zi de
2002, 23(2):30-32,57. DOI: 10.7671/j.issn.1001-411X.2002.02.009
Abstract:Nine kinds of fungal diseases bark rot,anthracnose, and seven kinds of leaf spots on Dracaena fragrans (L.) Ker Gawl. in Guangzhou region were investigated and identified in 1997 to 2000. The pathogens were Botryodiplodia theobromae Pat., Bartalinia dracaenaea P.G.Xi, Z.D.Jiang et P.K.Chi, Curvularia lunata (Walker)Boedijn, Curvularia senegalensis (Speg.) Subram., Glomerella cingulata (Stonem.) Spauld. et Schrenk, Pestalotiopsis clavispora (Atk.) Stey., Phomopsis dracaenae Sahni ,Phomopsis dracaenicola Z.D.Jiang, P.G.Xi et P.K.Chi, Sphaeropsis dracaenae P.G.Xi et P.K.Chi . Two severe diseases in the plantations of Dracaena fragrans were bark rot caused by B. theobromae and anthracnose caused by G. cingulata .
LI Yong bing , HUANG Hua zhi , HUANG Bing qiu , ZHAO Jing bin
2002, 23(2):33-36. DOI: 10.7671/j.issn.1001-411X.2002.02.010
Abstract:分别对湖南,湖北、广东、福建等稻区中的近20个地区稗草品系对二氯喹啉酸的抗性水平进行了系统鉴定,结果表明:湖南省安乡县稗草已对二氯喹啉酸产生了极明显的抗性,以50%芽长抑制中浓度IC50为标准,其相对抗性比值高达28.74%;湖南省常德市郊、汉寿县和湖北省黄梅县的稗草对二氯喹啉酸的抗药性也可能正在形成,其相对抗性比值在2.13-2.78之间;而广东省,福建省等一些地区的稗草尚未对二氯喹啉酸表现出抗性,用等浓度效果法和茎叶喷雾处理法也同样一致的得到了此结果。
YANG Qiong , XU Xing yao , LU Keng ming , ZHENG Xiang ming , FANG Ding jian
2002, 23(2):37-39. DOI: 10.7671/j.issn.1001-411X.2002.02.011
Abstract:从东方粉蝶成虫体中分离到一种卵圆形微孢子虫M Pc2 ,M Pc2孢子大小为 (3.38± 0 .35 ) μm× (2 .4 3± 0 .17)μm ,孢子双核结构 ,在家蚕体内可见到典型的Nosema型发育过程 ,认为M Pc2应归入Nosema属 .这种微孢子虫对家蚕具中度食下感染和弱胚种传染能力
LI Hong , KUANG Yan hua , PENG Xin xiang
2002, 23(2):40-43. DOI: 10.7671/j.issn.1001-411X.2002.02.012
Abstract:The effects of major nutrients in MS medium on suspension culture of sugarcane cell were studied, including different initial phosphate concentration, different nitrogen, total quantities of nitrogen, different sugar, different initial sucrose concentration. The results indicated that when the initial phosphate concentration was 1 25 mmol/L, the nitrogen was NH 4NO 3+KNO 3, the total quantities of nitrogen was 30 mmol/L, the sugar was sucrose and its initial concentration was 30 g/L, the growth of suspension culture of sugarcane cell was the best.
HE Ping , FU Xue lin , ZHAN Fu jian , ZHAO Ya hua , GAO Xiang yang , XU Shao jie
2002, 23(2):44-46. DOI: 10.7671/j.issn.1001-411X.2002.02.013
Abstract:When the original pH value of the penicillium's culture fluid was 5.5,the phytase was produced much .While the pH of culture fluid didn't decrease, the growth of penicillium could be inhibited. Calcium phytate could promote the penicillium to increase phytase activities.Two isoenzymes of phytase from penicillium were accepted by ammonium sulfate precipitation,sephadex G-100 gel filtration chromatography. The purification factors of phytase reached 3.3 folds with a specific activity of 71 3 U/mg.
WANG Sheng bin , ZOU Wei quan , YU Rang cai , FANG Sheng song , HUANG Zi ran
2002, 23(2):47-50. DOI: 10.7671/j.issn.1001-411X.2002.02.014
Abstract:用农杆菌介导的方法成功地将抗菌肽D基因导入到蕉柑胚性悬浮细胞,再生芽经PCR、Southern杂交证明抗菌肽D基因已整合到其基因组DNA中,在农杆菌介导的蕉柑胚性悬浮细胞转化过程中,共培养介质中的乙酰丁香酮(As)能显著提高其转化效率,与对照相比,每克悬浮细胞转化后形成的抗卡那霉素细胞团数从0提高到0.75,悬浮细胞与农杆菌共培养时间对转化效率也具有显著影响,共培养1、2、3d后,每克悬浮细胞转化后形成的抗卡那霉素细胞团数分别为0.20、0.75,0。
LUO Huan liang , XU Wei li , LI Jian zhong , FENG Qing liang , CUI Tang bin , HUANG Zhuo lie
2002, 23(2):51-53. DOI: 10.7671/j.issn.1001-411X.2002.02.015
Abstract:马占相恩(Acacia mangium)QLD19835家系树苗在人工气候室经低温处理后,未发生死亡,体内相关的生理指标,产生明显变化。随着温度不断下降至-6℃产生冻害导致植株的电导率大幅度升高,脯氨酸,可溶性糖和蛋白质的含量也显著升高,表明马占相思QLD19835树苗对低温胁迫有一定适应能力,脯氨酸,可溶性糖,蛋白质的含量提高可能是植株抗冷性的机制。
HUANG Jiu xiang , ZHUANG Xue ying
2002, 23(2):54-57. DOI: 10.7671/j.issn.1001-411X.2002.02.016
Abstract:The preliminary results on genetic diversity of Tsoongiodendron odorum at three sites of southern China were described. Eighteen 10 mer primers selected from 108 were applied for RAPD analysis. Seventy-eight genes were obtained from 42 leaf samples from the three populations, 66 of them (84.62% of total genes) were polymorphic. The result of cluster analysis showed that the population of Nankunshan of Guangdong province had the highest genetic diversity (3.074), the second was Jingxi of Guangxi province(2.389), the least was Duheng of Guangdong province (2 195). The similarity index between Nankunshan and Duheng in Guangdong province was the highest (0.832), that of Jingxi and Duheng was 0 772, and the similarty index between Jingxi and Nankunshan was 0 768.
ZENG Shu cai , XIE Zheng sheng , CHEN Bei guang
2002, 23(2):58-61. DOI: 10.7671/j.issn.1001-411X.2002.02.017
Abstract:The contents of elements B, Mo, Cu, Zn, Fe and Mn were studied in needles/leaves, twigs and fine roots of Chinese fir, masson pine, loblolly pine and blue Japanese oak. The contents and storages of these elements in the litters of four plantations were analyzed. The results showed that element B in needles/leaves and twigs of the forest trees were deficient, that contents of Mn were highly above normal level, and that those of the rest elements in needles/leaves and twigs and all those in fine roots were normal. The order of contents of the six elements in needles/leaves and twigs was Mn>Fe>Zn>B>Cu>Mo, while the order was Fe>Mn>Zn>Cu>B>Mo in fine roots. It was found that loblolly pine had the highest capacity to take up micronutrients. The order of microelements contents in litters was blue Japanese oak plantation>Chinese fir plantation>loblolly pine plantation>masson pine plantation, and the order of the total amount of the six microelements was masson pine plantation>loblolly pine plantation>blue Japanese oak plantation> Chinese fir plantation.
ZHOU Qing , XIAO Hong sheng , CHEN Bei guang , WANG Yan fang
2002, 23(2):62-64,73. DOI: 10.7671/j.issn.1001-411X.2002.02.018
Abstract:The landscape pattern of National Chebaling Nature Reserve was analyzed based on GIS by utilizing vegetation map. Results showed that forest type are the main component of vegetation in this region. The degrees of fragmentation of mid subtropical mountain evergreen broadleaf forest and mid subtropical hill and low mountain evergreen broadleaf forest were smaller. Pinus massoniana stand had the main impact on the formation of landscape pattern. The Shannon index of landscape diversity of type number was the biggest and of type area was the smallest.
2002, 23(2):65-69. DOI: 10.7671/j.issn.1001-411X.2002.02.019
Abstract:98只18日龄的粤黄公鸡随机分为7组,第Ⅰ组为对照组,每周每只皮下注射PBS0.5mL,其中第Ⅱ-Ⅳ组每周皮下注射3、6和12μg/kg禽胰多肽(APP)纯品,第Ⅴ-Ⅶ组每周同部位分别注射6、12和24μg/kg的APP粗品,连续7次,结果表明;注射高剂量粗品APP可提高鸡的采食量,提高饲料转化率,提高鸡只胴体质量和全净膛质量,血中FT3含量升高,鸡只生长速度加快,而一定量的纯品APP实际情况可促进鸡只生长,但不明显。
2002, 23(2):70-73. DOI: 10.7671/j.issn.1001-411X.2002.02.020
Abstract:在体外分别构建了3种重组表达质粒,含传染性囊病病毒GZ911株VP2基因的pGVP2、含HK46株VP2基因的pHVP2以及含HK46株5‘-端序列(包括非编码区,VP5和VP2基因)的pHVP2 5用不同的重组质粒转染质代鸡胚成纤维细胞(CEF),在转染后0、14、38h,收集对照组和转染组细胞,用酶联免疫吸附试验检测CEF中降解DNA量,结果,在14和38h,各组细胞中测得的Dλ值均为转染pHVP2 5组最大,其次为转染pHVP2,pCVP2的组,并都大于其他对照组,表明2个IBDV毒株的VP2蛋白在CEF中表达后都能诱导CEF发生凋亡,但不同毒株的VP2蛋白诱导CEF凋亡的能力不则;VP5蛋白可增强VP2蛋白诱导CEF凋亡的作用。
LI Yu-gu , ZHANG Yuan , HUANG Ren , CHENG Shu-jun
2002, 23(2):74-76. DOI: 10.7671/j.issn.1001-411X.2002.02.021
Abstract:The mast cells in the thymus, lymph nodes and spleen of rhesus monkey ( Macaca mulatta ) were observed by transmission electron microscopy and histochemical staining method using toluidine blue and alcian blue-safranin The results showed that they exhibited heterogeneity in shape, size, staining property and ultrastructure These mast cells varied in shape and size in the parenchyma and stroma of the thymus, in the stroma of lymph nodes and spleen, but were small and round in the parenchyma of the lymph nodes and spleen All the mast cells exhibited metachromasia with toluidine blue staining, but were almost all blue (alcian blue positive), rarely showed red (safranin positive) or mixed red-blue (double positive) with alcian-safranin staining Under transmission electron microscope, they were smooth or possessed a few processes on the surface, containing abundant organelles, also homogeneous secretory granules of high and moderate electron density in the cytoplasm
ZHAO Zuo xi , LUO Xi wen , MA Rui jun
2002, 23(2):77-80. DOI: 10.7671/j.issn.1001-411X.2002.02.022
Abstract:A positioning system incorporating absolute rotary encoder was developed so as to realize the automatic measurement and control of farming tools in test as well as soil processing equipment in soil bin. Microprocessor and non-volatile memory were used for data processing and communication with a PC. In this paper, theoretical analysis of the system was introduced and its hardware design also included. Some innovations in software design were developed and some new ideas were proposed consequently to accelerate the operation of the program and promote its efficiency considerably. In practical application this system showed higher precision and reliability.
2002, 23(2):81-83. DOI: 10.7671/j.issn.1001-411X.2002.02.023
Abstract:用异淀粉酶对淀粉进行切枝,研究了酶变性后淀粉糊的流变特性及其影响因素。结果表明:在ω(淀粉糊)为2%-6%,湿度25-70℃的实验条件下,酶解链淀粉呈现假塑性流体特征,具有触变性和剪切稀化性质,淀粉糊的链淀粉含量,浓度,温度等因素对其表观粘度有较大的影响。
GAO Zhen zhong , WANG Xiao bo , SUN Jin , SHEN Jia rui
2002, 23(2):84-85,92. DOI: 10.7671/j.issn.1001-411X.2002.02.024
Abstract:The influence of the molar ratio of formaldehyde to urea on free formaldehyde content (FFC) was studied in strong acid and weak acid. The optimal molar ratio for low FFC was found, and urea formaldehyde resin adhesive with URON structure and low FFC synthesized. The formaldehyde liberation was lower than E1 grade requirement when the adhesive was used in plywood produetion.
LE Xue-yi , FU Yin-lian , WANG Lei
2002, 23(2):86-88. DOI: 10.7671/j.issn.1001-411X.2002.02.025
Abstract:用pH电位滴定法研究了三元混配吡啶并[3,2-f][1,7]邻菲咯啉-Cu^2 -5′-三磷酸尿苷[Cu(pdphen)(UTP)^2-]配合物在水以及φ为30%,50%的二氧六环-水溶液中的稳定性(25℃,0.100mol/LNaClO4),将三元配合物与相关的二元配合物Cu(UTP)^2-的稳定性比较,发现三元配合物分子内存在着额外的稳定化作用,这可能主要归因于分子内配体UTP^4-磷酸基团上氧负离子与配体pdphen芳环间的σ-π协同作用以及UTP^4-尿嘧啶碱基与配体pdphen芳环间的堆积作用,用紫外差示光谱法确证了芳环间的堆积作用。
2002, 23(2):89-90. DOI: 10.7671/j.issn.1001-411X.2002.02.026
Abstract:苯基苯乙烯酮合成酶 (chalconesynthase ,CHS)是植物体内花色素苷等类黄酮化合物生物合成途径中的一个关键酶 .有关该酶特性及其基因表达调控在花发育过程中已有一些研究 ,大多集中于矮牵牛、金鱼草等模式植物[1] ,而对非洲菊等具有较高观赏价值的花卉研究较少 .非洲菊花序结构复杂 ,是研究花发育、花色素苷合成及花色素苷基因表达调控的理想材料 .非洲菊的头状花序可分为 3种类型的花 ,其最外层花 (rayflorets,Rf)大且色泽鲜艳 .本研究以非洲菊Rf为试材 ,提取不同发育时期Rf的总RNA ,通过测定花色素…
2002, 23(2):91-92. DOI: 10.7671/j.issn.1001-411X.2002.02.027
Abstract:森林枯枝落叶层是森林生态系统的重要组成部分 ,森林的众多生态系统服务功能[1] 并不单纯依赖于森林的立木部分 ,枯枝落叶层亦起着重要的作用 ,尤其是在涵养水源、保持水土以及促进森林生态系统物质循环和养分平衡方面 ,枯枝落叶层起着不可替代的作用 .因此 ,枯枝落叶层的研究对于生态公益林体系建设具有重要的理论和实践意义 .但目前 ,有关枯枝落叶层涵养水源方面的研究主要集中于对防护林体系的研究 ,如吴长文等[2 ] 及阎文德等[3 ] 的有关研究 ,少见对风景区森林枯枝落叶层进行研究 .本项研究对广州白云山风景名胜区 6种森林群落枯枝落叶…
2002, 23(2):93-93. DOI: 10.7671/j.issn.1001-411X.2002.02.028
Abstract:土壤养分、微生物和酶对林木生长有重要影响 .本文通过分析马占相思林和柚木林的土壤养分、微生物和酶活性状况 ,为合理利用土壤 ,科学造林提供依据 .1 研究方法 调查地位于华南农业大学林学院试验林地 (东经 113°18′ ,北纬 2 3°0 6′) ,属南亚热带季风气候。 1999年在马占相思林和柚木林中分别设置 2 0m× 2 0m的样地 ,测定样地内林木的胸径和树高 .用常规方法采取 0~ 4 0cm处的土样 ,带回实验室分析 .将水土以体积比为 2 5∶1混合后用pHS - 3C型酸度计测土壤pH ;有机质用重铬酸钾容量法测定 ;全N用半微量凯氏法测定 ;…
2002, 23(2):94-94. DOI: 10.7671/j.issn.1001-411X.2002.02.029
Abstract:立枯丝核菌 (RhizoctoniasolaniAG - 1IA)引起的纹枯病是水稻三大病害之一 ,关于化学肥料影响水稻纹枯病发生发展的报道很多 ,但化学肥料体外对病原菌的影响则很少报道 .本试验研究了氮、磷、钾对病菌菌丝生长和菌核形成的影响 .1 材料与方法 水稻纹枯病菌 (RhizoctoniasolaniAG - 1IA)保存于华南农业大学资环学院真菌研究室 .采用Czapek培养基及培养液 .氮肥为CO(NH2 ) 2 (北京南尚乐化工厂 )和 (NH4) 2 SO4(武汉制药厂 ) ;磷肥是NaH2 PO4·2H2 O(哈尔滨市新春化工厂 …
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