CHEN Yue jin , DING Xiao hua , ZHANG Gui quan , LU Yong gen
2002, 23(4):1-4. DOI: 10.7671/j.issn.1001-411X.2002.04.001
Abstract:Developing the indica compatible japonica lines (ICJLs) in rice may be an important procedure of overcoming the hybrid sterity to utilize the heterosis of F 1 hybrids between indica and japonica for raising rice grain yield. The heterosis of F 1 hybrids between the candidate ICJLs and indica varieties (lines) was identified. The results were as follows: 1.The F 1 hybrids between ICJL G3005 4 1 and indica varieties (lines) had great mid parent heterosis in the theoretic grain yield but the other indica compatible weak japonica lines and indica varieties (lines) had little mid parent heterosis. 2.The F 1 hybrids with great mid parent heterosis in the theoretic grain yield between ICJL G3005 4 1 and indica varieties (lines) had the great total grains and the great filled grains per panicle, normal seed set rate and proper growth duration and proper plant height. 3.The mid parent heterosis of F 1 hybrids in the theoretic grain yield was not correlated to parent average and the parent similitude coefficient identified by SSR(simple sequence repeat) markers at significant level.
CAO Pan rong , LIU Xian ming , GAO Fei die , WU Cheng , GAO Zhuo en
2002, 23(4):5-7. DOI: 10.7671/j.issn.1001-411X.2002.04.002
Abstract:The young shoot growth and chemical substances of fresh leaves of Dancong tea which were planted under 4 different microclimate's tea gardens were studied. The results indicated that variation of microclimate in tea gardens was more steady than nature tea garden in annual growth cycle. The high temperarure and strong sun light in summer were lowered; The temperarure was increased in cooler spring and winter, the humidity in dry autumn was raised. The microclimates with 80% & 60% shade net were confirmed that it promoted growth of tea plants,had a good shoot tenderness keep ability,reduced the content of polyphenols, increased the content of amino acid of fresh leaves and benefited to yield and quality of Dancong tea.
CHEN Fang yan , JI Ping xiong , WANG Lin chuan , PENG Wen fang
2002, 23(4):8-10. DOI: 10.7671/j.issn.1001-411X.2002.04.003
Abstract:The affection of different factors on the hydrolytic degree of silk fibroin peptide and the inhibiting ability formation of block pigmend of silkworm blood were studied while the silkworm cocoon was treated by gegumming, salt dissolution and alkali hydrolysis.The result showed that temperature had the most greatly influence to the hydrolytic degree of silk fibroin peptide, second factor was time, the concentration had the least influence. The inhibition ratio was only 28 34% when the silk protein was unhydrolytic, but it could rapidly reach to 48 95% when the silk fibroin was hydrolyzed. The highest value of the inhibition ability ratio for the formation of the black pigment was 67 14% while the hydrolytic degree of silk fibroin peptide was 0 476 5 g/L. Then, the inhibition ratio of the formation of the black pigment was reduced with the increase of the hydrolytic degree of silk fibroin peptide.
XIAN Ji dong , PANG Xiong fei , LIANG Guang wen , ZHOU Xian jun , FAN Wu
2002, 23(4):11-14. DOI: 10.7671/j.issn.1001-411X.2002.04.004
Abstract:By means of life table and Interference index of population control(IIPC), the effects of secondary substance of non preferable plant on Conopomorpha sinensis Bradley were studied in Shenzhen, Guangdong Province. The results showed that the alcohol extracts of Tephrosia vogelli, Eupatorium odoratum and Eucalyptus robusta which were selected from 24 species of plants had better repelling effect on adults ovipositting of Conopomorpha sinensis . When the alcohol extracts of Eupatorium odoratum, Tephrosia vogelli and Eucalyptus robusta spayed in the litchi orchard, the trend index of population of Conopomorpha sinensis in treatment were 2 817,3 206 and 3 863, respectively. The interference index of population control of Conopomorpha sinensis in treatment of the alcohol extracts of Eupatorium odoratum, Tephrosia vogelli and Eucalyptus robusta were 0 209, 0 238 and 0 286, respectively, while the fruit protective effects were 79 1%, 76 2% and 71 4%, respectively.
ZHAN Gen xiang , LIANG Guang wen , ZENG Ling
2002, 23(4):15-17. DOI: 10.7671/j.issn.1001-411X.2002.04.005
Abstract:研究了温度对美洲斑潜蝇(Lirimyza sativae Blanchard)的寄生蜂底比斯釉姬小蜂(Chrysocharis pentheus Walker)主要生命参数的影响,结果表明:在14-34℃的温度范围内,底比斯釉姬小蜂各虫态的发育历期随温度的升高而缩短,用最优法求得底比斯釉姬小蜂卵,幼虫和蛹的发育起点温度分别为10.06,10.29和8.56℃,有效积温为38.35,55.39和135.69日度,温度对寄生蜂蛹的体长,雌蜂的大小无明显影响,但高温和低温对各虫态的存活率影响较大,温度升高,寄生蜂成虫的寿命缩短。内禀增长率在30℃以下随温度的升高而加大,超过30℃则随温度的升高而降低。
2002, 23(4):18-22. DOI: 10.7671/j.issn.1001-411X.2002.04.006
Abstract:Different Brassica vegetable crops and oil seed Brassica crops were applied to observe the biological parameters of Plutella xylostella under laboratory condition. The larval, pupal duration, pupal mass, percent larval pupating, percent survival to adult and fecundity were recorded. The shortest larval and pupal period was recorded on Brassica camelina and Eurica sativus , and the longest larval and pupal period was recorded on cabbage Brassica oleracea var. capitata and Brassica oleracea var. botrytis . Percent larvae pupating varied significantly, and percent survival to adult stage was also varied significantly. The highest survival rate was found on cauliflower Brassica oleracea var. botrytis . Pupal mass on different host plants varied significantly, the lowest on Brassica napus and the highest was on E. sativus . The highest fecundity was observed on rocked seed fed female. Nitrogen content of different host plant leaves were also varied considerably and had obviously correlative with the pupal mass and the fecundity.
ZHANG Wei min , WANG Zhen zhong
2002, 23(4):23-26. DOI: 10.7671/j.issn.1001-411X.2002.04.007
Abstract:Based on the results of field investigation in spring and autumn tomato planted fields in Guangdong province in 1998, a detail analysis was conducted for spatial distributions of the diseased plants and its aphid vectors (Myzus persicae ) in autumn with several indices of spatial pattern. Spatial distribution analysis of the disease showed that the distribution patterns of diseased plants in the field were changed with the time passing. At the beginning of the disease developping, a random pattern was observed, then a clustering type of distribution, and an even pattern at the last stage of the plant. Further analysis of the aphid vectors in autumn crop showed that the alatae always had a clustering type of distribution. It was showed in the present study that the distribution of alatae was similar to that of the diseased plants, and suggested that the alatae were the main effective vectors of the disease.
ZHAO Dong xiang , GAO Jing lin , CHEN Zong mao , CHENG Jia an , XU Han hong
2002, 23(4):27-29. DOI: 10.7671/j.issn.1001-411X.2002.04.008
Abstract:: The results from the study on the olfactory orientation behavior of Empoasca vitis to tea plant showed that the greenfly lefhopper Empoasca vitis inclined firstly to light, which was the role of visual sense, and they showed response to ordor. when neared to the host plant from the certain distance, Tea shoots volatiles and linalool showed the strongest attraction to E.vitis, and the compound 2,6 dimethyl 3,7 octadiene 2, 6 diol resulted from tea plant greenfly leafhopper complexes, showed slight repellent role.
YANG Cun yi , CHEN Le tian , CHEN Fang yuan , LIU Yao guang
2002, 23(4):30-33. DOI: 10.7671/j.issn.1001-411X.2002.04.009
Abstract:调查了用细胞质雄性不育新恢复系ZSP-1配制的杂交组合珍汕97A/ZSP-1和星A-ZSP-1的F1的结实率及F2个体花粉育性,发现恢复系ZSP-1的恢复性是由2对独立遗传基因控制。在此基础上,选用了与野败型雄性不育恢复基因Rf-3和Rf-4紧密连锁的RFLP标记对2个F2群体进行连锁分析,发现ZSP-1具有的2个恢复基因与这些标记紧密连锁,初步把ZSP-1的恢复基因定位于Rf-3和Rf-4区域。
HU Kai lin , FU Qun mei , WANG Guo ping , HU Zhi qun
2002, 23(4):34-36. DOI: 10.7671/j.issn.1001-411X.2002.04.010
Abstract:通过根癌农杆菌介导,采用叶盘转化法,探讨了黄瓜花叶病毒外壳蛋白(CMV-CP)基因导入番茄栽培品种‘红宝石’的适宜条件。结果表明:适宜的卡那霉素(Km)浓度,根癌农杆菌浓度,根癌农杆菌感染时间以及共培养时间分别为35mg/L,D600约0.5,10-15min和2-3d;将诱导形成的抗性愈伤组织转接到含有35mg/L Km的分化培养基和生根培养基中使其分化不定芽和生根,获得了抗Km的番茄再生植株。对再生植株进行再欠离体培养抗Km鉴定和PCR分子检测,补CMV-C基因已导入番茄再生植株的基因组中。
LIANG Ji nan , TAN Zhong wen , TAN Zhi yong , ZHENG Zi sheng , KONG Chui hua , HU Fei
2002, 23(4):37-40. DOI: 10.7671/j.issn.1001-411X.2002.04.011
Abstract:Properties of tissue culture of 11 sugarcane genotypes were studied. The results showed that the ratios of explant browning were obviously decreased and the ratios of callus inducing were greatly increased after the explants were soaked in 200 mg/L polyvinylpyrrolidone(PVP) solution for 20 minutes. The ratios of explant browning, callus inducing, callus multiplication and callus differentiation of 11 sugarcane genotypes were significantly different in the same culture condition.
ZENG Shu cai , XIE Zheng sheng , GU Yan kun , SU Zhi yao , CHEN Bei guang , LIN Shu hao
2002, 23(4):41-44. DOI: 10.7671/j.issn.1001-411X.2002.04.012
Abstract:A preliminary study on the biomass,forest litter remnant amounts and water holding capaities of five forest communities of the Baiyunshan Scenic Spot,Guangzhou showed that the order of community biomass was Acacia mangium Schima superba>Pinus massoniana>Acacia mangium>Castanopsis fissa>Pinus caribaea and that the order of litter remnant amounts was Pinus massoniana>Castanopsis fissa>Acacia mangium Schima superba>Acacia mangium>Pinus caribaea . Differences in natural water holding capacities of the different strata of the communities were remarkable and the capacity order was soil stratum>tree stratum>shrub herb stratum>litter stratum,with the exception of Pinus massoniana where shrub herb stratum>tree stratum. The community of Acacia mangium Schima superba had the highest total natural water holding capacity,followed by Acacia mangium,Pinus massoniana,Pinus caribaea and Castanopsis fissa in a decreasing sequence.
YIN Ai guo , SU Zhi yao , CHEN Bei guang , LIU Hui ming
2002, 23(4):45-48. DOI: 10.7671/j.issn.1001-411X.2002.04.013
Abstract:Floristic composition of the evergreen broadleaved forest in Baiyunshan, Guangzhou, was analyzed based on community survey data. Field survey indicated that the community was rich in species diversity, with 53 families of vascular plants including 85 genera and 103 species. Dominant families of the flora were Lauraceae?Rubiaceae?Rutaceae? Euphorbiaceae? Moraceae?Aquifoliaceae and Fagaceae, most of which are tropical and subtropical. As for generic distribution, the Pan tropic areal type has the majority of genera, followed by Tropical Asia and Old World Tropics, while typical tropical genera are rare. Floristic composition of the evergreen forest community in Baiyunshan and the characteristic species of the flora epitomizes the zonal flora, which will provide scientific foundation for stand rehabilitation and stand establishment approximating to the natural forest community. This study also indicates that the sampled data based on community research are valid and convenient for floristic analysis.
FENG Zhi jian , LI Zhen kui , WU Yong bin , LIU Jian bin
2002, 23(4):49-52. DOI: 10.7671/j.issn.1001-411X.2002.04.014
Abstract:A total of 1 549 species and varieties of vascular plants belonging to 745 genera, 204 families were found in Lianhuashan Baipanzhu Nature Reserve, Huidong, Guangdong Province.Of them, 104 were fern species falling into 52 genera, 30 families, 21 gymnosperm species belonging to 16 genera, 10 families and 1 424 angiosperm species belonging to 677 genera, 164 families. Among the plants found, 54 species were national key protected plants, 606 were medicinal plants, 196 ornamental ,173 timber, 111 fiber, 103 fruit, 89 oil bearing, 66 fodder, 55 tannic, 39 potherb and 36 pesticide.
YANG Yan qiong , CHEN Yi gang , HUANG Ping , ZHANG Wei liang , HOU Chang mou , DENG Jian feng
2002, 23(4):53-56. DOI: 10.7671/j.issn.1001-411X.2002.04.015
Abstract:Forest resources of Baiyun District were investigated based on RS and GIS.Results showed that main forest was classified with computer automatic classification in Guangdong province. The classification accuracy was 94 44%; The area data of image classification was fairly close to those of second class investigation in each town.
XIE Shao-feng , SU Zhi yao , LIN Wei qiang
2002, 23(4):57-60. DOI: 10.7671/j.issn.1001-411X.2002.04.016
Abstract:Ecotourism is a new kind of tourism, in which plants are important resourses. In this paper, the roles of rare and endangered plants, special dominant plants and typical zone vegetations and plants with special shape, specific utilities, or deep cultural contents in ecotourism were discussed. The strategies on how to use and protect the plants were proposed.
2002, 23(4):61-63. DOI: 10.7671/j.issn.1001-411X.2002.04.017
Abstract:根据现有鸡γ-干扰素基因序列设计引物,应用反转录-聚合酶链反应(RT-PCR)技术,从Con A诱导培养的鸡脾淋巴细胞中扩增得到石岐杂鸡γ-干扰素(spzChIFN-γ)基因,将克隆到pGEM-T载体中,进行序列测定,结果表明,克隆得到的ChIFN-γ基因的开放阅读框架由492个核苷酸组成,和其他ChIFN-γ基因的同源性达99%,与火鸡γ-干扰素基因的同源性性为96%,与鸭γ-干扰素基因的同源性为81%,推导的石岐杂鸡IFN-γ氨基酸序列与其他已发表的ChIFN-γ氨基酸序列完全一致,与火鸡和鸭IFN-γ氨基酸同源性分别为97%和67%。
WANG Chun xia , WANG Lin chuan , HUANG Ai fang , WANG Qing , YAN Zhi qiang , CHEN Qing hua
2002, 23(4):68-70. DOI: 10.7671/j.issn.1001-411X.2002.04.019
Abstract:从经免疫的番鸭外周血分离淋巴细胞,经PHA刺激培养后提取总RNA,采用RT-PCR的方法,按照GenBank中序列号为X84764鸭I型IFN(DIFN1)基因设计引物,成功扩增出番鸭IFN基因,命名为MDIFN-α,包含编码MDIFN-α成熟蛋白全部核苷酸序列。DIFN1开放阅读框架(ORF)有576个核苷酸,编码191个氨基酸,其中切除信号肽的IFN-α为成熟DIFN1,共有486个核苷酸组成,编码161个氨基酸。MDIFN-α(含有不完整的信号肽)与X84764的核苷酸同源性为97.7%,推导氨基酸同源性为95.7%,结果显示,MDIFN-α可能为鸭I型IFN一个新的亚型。
SHI Ying xin , LUO Man lin , HUANG Yu mao , LIU Zhen ming
2002, 23(4):71-74. DOI: 10.7671/j.issn.1001-411X.2002.04.020
Abstract:A pair of primers were designed and synthesized. Using the whole genomes of strain PVR MinA and PRV YueA as templates,specific products were obtained as expected by PCR amplification and cloned into pUCm T vector.Recombinants were confirmed by colony PCR and restriction enzyme digestion.The inserts were sequenced,and the results revealed that each of both inserts composed of 558 neucleotides,coding for 186 amino acids.Comparison of the target genes showed 96 9% nuleotide sequence homology and 92.5% amino acid sequence homology between strain PRV YueA and PRV MinA,which is relatively low.
LIU Di jie , SHI Wei , ZHUANG Wei dong , FENG Qi hui
2002, 23(4):75-77. DOI: 10.7671/j.issn.1001-411X.2002.04.021
Abstract:Staphylococcus aureus [1 mL 10 6 cfu (colony forming unit)/mL] suspension was infused into one side of udder chamber of the healthy lactating milky goats. Mastitis model was identified and determined by such indices as clinical symptoms, body temperature, somatic cell count in milk, electronic mastitis detector, pathogen isolation and cultivation, and histopathological changes. The results showed that acute mastitis model can be duplicated by inoculating the above amount of bacteria in one side of udder chamber of lactating goats.
ZHAO Xin , LUO Xi wen , REN Yue gang , OU Ying gang , HONG Tian sheng
2002, 23(4):78-80. DOI: 10.7671/j.issn.1001-411X.2002.04.022
Abstract:通过 4个实验测试了Magellan公司ProMARKⅩ接收机的定位精度 .结果表明 ,采用事后伪距差分定位法 ,该机固定点动态定位误差小于 2 86m ,短基线距离测量平均相对误差为 6 6 5 % ,定位精度良好 ,可满足精细农业的要求 .
2002, 23(4):81-83. DOI: 10.7671/j.issn.1001-411X.2002.04.024
Abstract:A microcosmic mechanics model was established and used to describe interface combination status of particle board. Shearing stress and direct stress were separated by limited unit law analysis. It's demonstrated that the interface destruction was due to shearing stress and that the end of glue layer interface of pulled particle was the weak point of combination.
CHEN Min , YANG Le min , LUO Zhi gang , LU Qi ming
2002, 23(4):84-86. DOI: 10.7671/j.issn.1001-411X.2002.04.025
Abstract:The intercalation composite of montmorillonite/polyacrylamide was synthesized using the activation of interlayer montmorillonite. The intercalated composite was characterized by X ray diffraction, fourier transform infrared absorption spectrum and differential thermal analysis.
2002, 23(4):89-89. DOI: 10.7671/j.issn.1001-411X.2002.04.027
Abstract:菜心 (BrassicaparachinensisBail.)离体培养时 ,由于基因型的差异 (AA基因型 )较其他芸薹属作物更为困难 ,植株再生频率仅为 10 %~ 30 % [1] .离体培养的主要问题是愈伤组织分化频率低 ,在分化成苗时先产生根而抑制了芽的分化[1,2 ] ,芽伸长困难 ,实验结果重复性差 ,不能满足遗传转化的需要 .本实验研究了不同外植体、培养基附加成份等因素对菜心离体培养植株再生和分化频率的影响 ,以AgNO3 和GA3 相配合 ,获得了高频率、芽壮且多的再生植株 ,对保存种质、扩大繁殖和遗传转化有一定的意义 .1 材料与方…
2002, 23(4):90-90. DOI: 10.7671/j.issn.1001-411X.2002.04.028
Abstract:评价单质或复合肥质量的主要指标是肥料养分含量 ,而评价控释 /缓释肥技师的主要指标是养分的控释 /缓释性能 ,其测定方法主要为水中溶出率法和土壤淋出率法 ,但适用范围和对结果的解释尚未统一 .笔者研究了几种控释 /缓释肥在水、土壤、砂和具有不同CEC值的土壤 +砂、土壤 +沸石介质中的氮素释放特性 ,说明了土壤因子对评价控释肥的意义 .1 材料与方法肥料 :Osmocote (14 - 14 - 14 ) :包膜复合肥 ,荷兰生产 ;Luxecote (2 0 - 6 - 10 ) :包膜复合肥 ,深圳市芭田复合肥有限公司生产 ;Lujin :(41- 0 - 0 ) :包膜尿…
2002, 23(4):91-92. DOI: 10.7671/j.issn.1001-411X.2002.04.029
Abstract:绿巨人 (SpathiphyllumpallsHort.)是天南星科苞叶芋属植物 ,植株形态优美 ,耐阴 ,适合长期在低光照强度条件下摆放 ,因而已成为美化居室的一种重要观叶植物 ,生产上有较大面积栽培 .尽管对观叶植物的一些生理生化特性已有一些研究[1~ 4] ,但对绿巨人的需肥特性研究很少 ,因而生产上施肥比较盲目 .若施肥水平与植物生长的关系不明 ,一方面造成肥料的浪费 ,增大生产成本 ;另一方面 ,还造成化肥对环境生态的破坏 .因此 ,加强这方面的研究 ,对于指导生产实践 ,提高经济效益及降低环境污染很有意义 .本文以绿巨人为材料 …
2002, 23(4):93-94. DOI: 10.7671/j.issn.1001-411X.2002.04.030
Abstract:地理信息系统 (geographyinformationsystem ,GIS)是利用现代计算机图形学和数据库技术输入、存储、编辑、查询、决策和输出空间图形及其属性数据的计算机系统[1] .它是融合地理学、几何学、计算机科学及各类应用模型为一体的高新技术 .今天 ,人们开发GIS的软件也越来越多 ,有工具型GIS开发软件 :MapEngine、ARC/INFO、MapinfoProfessional、GenaMap、GeoStar等 ;有面向对象可视化程序设计语言 :VisualBasic、Vi sualC ++、Pow…
You are the visitor
Mailing Address: GAddress:No.483 Wushan Rd, Tianhe,Guangzhou 510642, Guangdong Province, China Post Code:510642 Phone:(020)85280069/38746672