CHEN Zhao-gui , WANG Jiang , ZHANG Ze-min , LIU Fang , ZHU Hai-tao , WAN Xin-shan , ZHANG Jing-liu , ZHANG Gui-quan
2006, 27(1):1-4. DOI: 10.7671/j.issn.1001-411X.2006.01.001
Abstract:在筛选和鉴定水稻T-DNA(Ds)插入纯合体的过程中,观察到1个叶片卷曲的突变体.对该突变体进行遗传分析表明,分离群体出现叶片卷曲和叶片正常2种植株类型,其分离比率为3:1,符合1对基因的显性遗传.Basta抗性检测及PCR分子检测证实,该突变体是由单一T-DNA(Ds)插入所引起的,突变性状与T-DNA(Ds)共分离.该突变材料可用于插入座位的基因克隆.
GAO Lu , HU Chun-sheng , CHEN Su-ying
2006, 27(1):5-8. DOI: 10.7671/j.issn.1001-411X.2006.01.002
Abstract:Based on soil moisture distribution under sprinkler irrigation,the root growth and distribution of winter wheat under different irrigation conditions were studied.The results show that sprinkling irrigation is more useful for the plant's growing and can also increase the efficient utilization of water.Under sprinkling irrigation,the root of the plant concentrates on the upper soil and tends to move up.Both of jointing stage and ripening stage, the maximum of root length density apprears on the 20 cm layer and root length density decreases with the increases of the depth under 20 cm.The root's death rate is different under different water treatments.The death of the root is mainly occured on the surface of soil.When analyzing the root dry mass of different layers of the three treatments in jointing and ripening stages,the difference isn't significant.
CHEN Hou-bin , FENG Qi-rui , XU Chun-xiang , HE Ri-xiang , LI Jian-guo , WANG Ze-huai
2006, 27(1):9-12. DOI: 10.7671/j.issn.1001-411X.2006.01.003
Abstract:枯萎病Fusarium oxysporum f.sp.cubense(FOC)近年成为华南地区香蕉产业的最大威胁.为了筛选抗病种质,于2002~2004年期间分别在广州番禺区对15个引进品系和1个主栽品种进行了抗性评估和筛选.根据评估结果,供试品系可归为3类:抗病——FHIA-02,FHIA-03,ev.Rose,FHIA-23,GCTCV-119,FHIA-2l;中抗——CRBP39,TMBx5295-1,FHIA-18,SH-3640,SH3436-9;感病——Williams,FHIA-17,Gros Michel和巴西蕉.Williams和GCTCV-119均为高型香蕉(Giant Cavendish)的选系,前者被作为FOC感病的参考品种,但在第二茬18株中发现2株变矮(1.8m对2.5m)未发病.
HU Gui-bing , ZHANG Shang-long , XU Chang-jie , LIN Shun-quan
2006, 27(1):13-16. DOI: 10.7671/j.issn.1001-411X.2006.01.004
Abstract:Codon DNA sequence(CDS) of four different citrus species,including sweet orange(Citrus sinensis),satsuma mandarin(C.unshiu),grapefruit(C.paradisi) and lemon(C.limon) were used to analyze the relative frequency of synonymous codon(RFSC),and high-frequency codons were selected by high-frequency codon analysis.The results indicated that the codon preference was little different in four citrus species.
CAO Pan-rong , LIU Chun-yan , LIU Ke-bin , YU Xiong-hui
2006, 27(1):17-20. DOI: 10.7671/j.issn.1001-411X.2006.01.005
Abstract:Effect of the different drought stress on the aromatic constituents and relative contents in the fresh leaves of Lingtou Dancong tea plants was studied in this paper.Results showed that drought stress could evidently increase the kinds of aromatic component in fresh leaves.The largest number(58 kinds) of aromatic constituents was found under 10.56%(w) water stress among several treatments,while the fewest number was found under the absolute water content of 19.59%(w).The total amount of relative content of 17 kinds of aromatic constituents as Linalool etc.,increased with drought stress,whereas 12 kinds of aromatic constituents as Tetradecanoic acid etc.,decreased with drought stress.Linalool,Linalool oxide,Etradecane,10-Methylnonadecane,Dodecanal showed highest contents under the absolute water content of 10.56%(w);Cyclohexane,1-Hexadecene,1-Tricosanolonly was induced in the absolute water content of 10.56% and 5.73%(w),while drought stress could inhibit synthesis of 7 kinds of constituents as nonanedioic acid monomehtyl ester,etc.The different drought stress could induce special aromatic constituents.The number of aromatic constituents in fresh leaves increased with drought stress.
LI Hai-ying , ZHANG Xue-jun , ZHANG Shu-ting , ZHAO Xin-hua
2006, 27(1):21-24. DOI: 10.7671/j.issn.1001-411X.2006.01.006
Abstract:研究了以污水厂污泥与尿素、磷酸二铵、磷酸铵、氯化钾等化肥为原料的复混肥生产工艺,通过控制C/N、含水率、温度、供气量等工艺参数,生产出了能在农业上应用的污泥复混肥.通过在沙质土壤中施用不同量及不同配方的复混肥,进行了城市生活污水污泥复混肥在冬小麦生长中的应用效果研究.结果表明,污泥复混肥具有良好的改良土壤、增加小麦产量的作用.污泥复混肥作底肥与对照组相比,对植株的生长性状大都产生了显著或极显著的影响.小麦冬前分蘖增加30~60万个/hm^2,返青后分蘖数增加30~90万个/hm^2,拔节期蘖茎数增加30~105万个/hm^2,穗数增加19.5~45万条/hm^2,株高增加2~4cm.施用复混肥对小麦发育生长没有不良影响,有增产效果.一般穗粒数增加1~2个,千粒质量提高0.1~1.7g,产量增加457.5~1209.0kg/hm^2,增产率为7.6%~20.2%.但是由于污泥中重金属在土壤中的累积及在粮食作物中的富集现象,施用复混肥应控制污泥的比例.
2006, 27(1):25-28. DOI: 10.7671/j.issn.1001-411X.2006.01.007
Abstract:A pot experiment with tomato cv.Hongbaoshi grown on perlite and soil receiving liquid steeped chicken manure and liquid steeped peanut cake applied through drip irrigation system was conducted to find out the feasibility of this new application method of the two manures.As compared with tomato specific nutrient solution,under perlite culture,fruit yield of liquid chicken manure and peanut cake treatments was only 64.5% and 82.9% of that of nutrient solution,respectively.Under soil culture,fruit yield of liquid chicken manure and peanut cake treatments was 147.0% and 82.8% of that of nutrient solution,respectively.No significant difference of single fruit mass,contents of total soluble solids and titratable acids of fruits was observed among treatments. The dregs was 3.5% when chicken manure was steeped and most of the liquid could be injected into drip irrigation system.However,there was much more dregs(accounting for 74.0%) when peanut cake was steeped which made it unsuitable to be used in drip irrigation system.
S.N. MANDOUR , QIU Bao-li , REN Shun-xiang , WEN Shuo-yang , S.N. MANDOUR
2006, 27(1):29-33. DOI: 10.7671/j.issn.1001-411X.2006.01.008
Abstract:The differentiation of three haplotypes of Bemisia tabaci populations in China was analyzed using RAPD-PCR in this study.Five B.tabaci populations were collected in the north,middle and south of China,respectively,including three B biotype populations,the indigenous haplotype populations(NaC haplotype) and a new haplotype population from ornamental plants(C_v haplotype).In addition,the greenhouse whitefly,Trialeurodes vaporariorum,was used as a outgroup population in this analysis.The results of the cluster analysis using genetic distances indicated that,first,the population relationship between the NaC haplotype and the C_v haplotype was more close than that between B biotype and NaC haplotype,or that between B biotype and the C_v haplotype;second,the population relationships of the three haplotype of B.tabaci in China was intraspecific,and the C_v haplotype was potentially originated in China or the neighboring countries.
XU Yi-juan , LU Yong-yue , ZENG Ling , XI Yin-bao , HUANG Jun
2006, 27(1):34-36. DOI: 10.7671/j.issn.1001-411X.2006.01.009
Abstract:The location expansion of the red imported fire ant, Solenopsis invicta Buren,in a special ecology system were investigated and analyzed.The result showed the square area of the infestation was up to(226 hm~2) about 8 years after it was introduced to this area;Expansion distance were as follows: it spread to upper and lower reaches with the distance of(262) and(3 770 m) respectively.Active mounds densities were different among different types of habitat,with field bank>riverbank>roadside>village >riverbed. Average expansion speed of red imported fire ant was(187.9 m/year) in this area.
LUO Shu-ping , ZHANG Yong-qiang , HUANG Shou-shan
2006, 27(1):37-40,46. DOI: 10.7671/j.issn.1001-411X.2006.01.010
Abstract:The niche of major predatory arthropod on different resistant rice planthopper in three paddy fields were studied, the results showed that Ulmmeliata insecticepes was the key predator of rice planthopper in early rice. The spatial niche breadth of Dyschiriognatha dentata on resistant variety was the highest while that of Staphylinidae was the highest on susceptible variety. The temporal and temporal-spatial twodimension niche overlap value of predatory arthropod to rice planthopper on medial resistant variety was higher than that on resistant rice planthopper and susceptible variety. The predatory of medial resistant variety was more effective to control rice planthopper.
LIU Guang-hua , ZENG Ling , LIANG Guang-wen , ZHANG Mao-xin
2006, 27(1):41-43. DOI: 10.7671/j.issn.1001-411X.2006.01.011
Abstract:Community component and damage of rice borers were systematically investigated in rice fields in Guangdong Province.The results showed that four major rice stem borers were existed in Guangdong rice fields.They were Scirpophaga incertulas(Walker),Chilo suppressalis(Walker),Chilo auricilia Dudgeon and Sesamia inferens(Walker).Yellow stem borer distributed all over rice grown region in Guangdong Province as reported before and occurred more seriously in Taishan,Nanxiong and Xinhui three counties.It was found that the spread trend of striped stem borer was from northern to southern counties and stalk stem borer distributed all over the rice field in the province except Nanxiong county.The rice loss caused by the rice stem borers was not serious in Guangdong Province because of chemical and cultural control.
2006, 27(1):44-46. DOI: 10.7671/j.issn.1001-411X.2006.01.012
Abstract:Belonging to the north part of Taihang Mountains,Xiaowutaishan is located at the north Hebei Province.Through a field collecting trip in the summer of 2005,five species of the genus Carabus were collected from Xiaowutaishan National Nature Reserve: Carabus(Aulonocarabus) canaliculatus Adams,C.(Pagocarabus) crassesculptus Kraatz,C.(Morphocarabus) latreillei Fischer,C.(Oreocarabus) vladimirskyi Dejean and C.(Scambocarabus) sculptipennis Chaudoir.Among them,the latter three were newly discovered from the nature reserve.
AO Shi-en , YANG Mei , ZHOU Er-xun , TANG Qian-fei , PAN Ru-qian
2006, 27(1):47-50. DOI: 10.7671/j.issn.1001-411X.2006.01.013
Abstract:以水稻成熟胚培养产生的愈伤组织和悬浮细胞为筛选材料,以广东省水稻纹枯病菌Rhizoctonia solaniKühn强致病力菌株GD-11和GD-118产生的粗毒素为选择压力,从水稻品种的选择、粗毒素致病力的鉴定、突变体的筛选方法、突变体的再生以及突变体再生植株对粗毒素的抗病性鉴定等方面展开研究,建立了水稻抗纹枯病突变体的筛选体系并获得了抗病突变体.筛选体系为:两步筛选法,即以粤香占的愈伤组织和悬浮细胞为筛选材料,以φ(粗毒素)为3.5%和4.0%分别作为第1、2步的处理浓度,每一步处理的时间为20 d;筛选出的突变体再生苗的病情指数为37.55,而原始型再生苗的病情指数为87.50,抗病效果达57.08%.
XU Dong-lin , ZHOU Guo-hui , SONG Xiao-bing
2006, 27(1):51-54. DOI: 10.7671/j.issn.1001-411X.2006.01.014
Abstract:对侵染广东省阳西县田间辣椒的黄瓜花叶病毒(cucumber mosaic wirus,CMV)的CP及2b基因进行序列分析,结果表明其CP基因与CMV亚组Ⅰ、亚组Ⅱ各株系之间的核苷酸同源率分别为90.9%~93.8%和76.1%~76.9%。氨基酸同源率分别为92.7%~97.7%和72.4%~78.1%;其2b基因与CMV亚组Ⅰ、亚组Ⅱ之间的核苷酸同源率分别为83.9%~90.2%和64.0%~67.7%,氨基酸同源率分别为79.3%~85.7%和50.5%~53.5%,由此确认该CMV分离物属于亚组Ⅰ成员.此外,CMV亚组Ⅰ成员的CP及2b基因中分别具有一段长为102bp及54bp的高度保守序列,这为转基因诱导寄主转录后基因沉默(post-transcriptional gene silencing,PTGS)CMV育种研究提供了参考.
WENG Qun-fang , ZHONG Guo-hua , WANG Wen-xiang , LUO Jian-jun , HU Mei-ying
2006, 27(1):55-60. DOI: 10.7671/j.issn.1001-411X.2006.01.015
Abstract:采用浸渍生测法测定了采自广东、新疆、湖南的15科28种植物提取物对南方根结线虫Meloidogyne incognita的毒杀活性.结果表明:骆驼蓬Peganum harmala、黄文江鱼藤Derris elliptica和火殃勒Euphorbia antiguontm甲醇提取物以1000μg/mL处理后48h,供试2龄线虫校正死亡率分别为86.46%、69.28%和70.84%,处理后72h,校正死亡率分别为98.20%、85.45%和81.90%,呈强杀线虫活性.此外,厚果鸡血藤Millettia pachycarpa、桃花心木Swietenia mahagany等12种植物甲醇提取物亦有较强的杀线虫活性.盆栽试验结果表明:骆驼蓬、黄文江鱼藤、厚果鸡血藤、桃花心木提取物处理的番茄地上部植株高度分别比对照增长163.35%、90.61%、155.99%和178.08%,地上部鲜质量分别增长191.37%、130.25%、79,25%和124.35%.骆驼蓬、黄文江鱼藤、桃花心木、火殃勒提取物处理后根长分别增长140.57%、93.40%、135.85%和107.55%,均显著优于或相当于对照药剂阿维菌素(30μg/mL,即6%乳油2000倍)处理增长率98.11%.黄文江鱼藤提取物处理后根结数2.33个/株,病级最低(1.00),显著低于印楝素2.5μg/mL处理.骆驼蓬、黄文江鱼藤、桃花心木、火殃勒对盆栽番茄南方根结线虫病控制效果明显.
CHEN Chun , XIE Hui , WEI Ying-jun , HAN Yu-chun , QIN Dan , HUANG Chun-xiao , TANG Jiong
2006, 27(1):61-64. DOI: 10.7671/j.issn.1001-411X.2006.01.016
Abstract:以10g/L琼脂粉培养基为对照,研究筛选了新的培养基.新筛选的100g/L马蹄粉培养基、100g/L玉米粉培养基和100g/L菱角粉培养基可以培养用于繁殖香蕉穿孔线虫的胡萝卜愈伤组织.其中100g/L马蹄粉培养基和100g/L玉米粉培养基培养的胡萝卜愈伤组织繁殖香蕉穿孔线虫的效果较好,而100g/L马蹄粉培养基培养周期短、速度快、成本低,可以用于代替琼脂粉培养基培养胡萝卜愈伤组织来繁殖香蕉穿孔线虫.香蕉穿孔线虫不同种群的繁殖力存在明显差异,本研究的10个种群中,来源于红掌的RH1种群的繁殖力最强.
SHU Wei , HE Li-ping , CUI Tang-bing , GUO Yong
2006, 27(1):65-68. DOI: 10.7671/j.issn.1001-411X.2006.01.017
Abstract:采用活化的单甲氧基聚乙二醇(mPEG-5000)对木瓜凝乳蛋白酶(Cp)进行了共价修饰,修饰产物经毛细管电泳检测与分析表明:主产物为平均每分子Cp偶联3—4个mPEG。长链的低修饰度修饰酶.以此修饰酶为研究对象,进行了Cp在修饰前后的主要酶学、药物生物学性质的比较研究.结果表明:Cp经mPEG,修饰后,Kn^app(酪蛋白为底物)有所增加,但与Cp相比,mPEG1修饰酶的pH稳定性、热稳定性以及抗胰蛋白酶的水解能力却均有增强;药代动力学结果还显示,mPEG1-Cp的体内活性半衰期延长,修饰酶体内半衰期是原酶的1.6倍,表明mPEG1修饰酶具有更好的临床应用前景.
ZHANG Ming-sheng , QI Jin-liang , DU Jian-chang , YANG Chun-xian , YANG Yong-hua , TAN Feng
2006, 27(1):69-71,75. DOI: 10.7671/j.issn.1001-411X.2006.01.018
Abstract:对水分胁迫下甘薯植株质膜相对透性(RPP)和水分状况与品种抗旱性关系的研究结果表明,水分胁迫下不同甘薯品种叶片的RPP和水分饱和亏(WSD)均明显增大,叶片相对含水量(wRW)、自由水与束缚水含量比值(w1/wb)及藤叶与块根含水量比对照均不同程度下降.wRW与品种抗旱性呈极显著正相关(r=0.9080,P〈0.01),RPP、WSD、w1/wb及块根含水量的相对值与品种抗旱性均呈极显著负相关(r=-0.6797~-0.8937,P〈0.01),藤叶含水量的相对值与品种抗旱性问的相关性不显著(r=-0.3675,P=0.1778).因此,除藤叶含水量外,其余指标均可用于甘薯品种抗旱性的评定.
2006, 27(1):72-75. DOI: 10.7671/j.issn.1001-411X.2006.01.019
Abstract:以紫罗兰Matthiola incana无菌苗下胚轴为材料,研究了光照条件、无菌苗日龄、酶液渗透压、酶液组合及酶解时问对其原生质体分离效果,结果表明,种子在20℃黑暗下萌发4d后,转移到2000lx、每日光照14h条件下,约14d苗龄,用埘为1,2%的纤维素酶(celluase onozuka R-10)+w为0.8%的离析酶(macerozyme onozllka R-10)+3mmol/LMES+CPW-10M(CPW+w为10%的甘露醇)酶组合处理约12h,可以高效分离出有活力的原生质体.
HE Li-hong , CAI Ma , HE Sheng-gen , LAN Xia , ZHENG Yi-xiong , XIONG Xing-chang , LAN Shi-wei
2006, 27(1):76-78. DOI: 10.7671/j.issn.1001-411X.2006.01.020
Abstract:Polyamine oxidase(PAO) and peroxidase(POD) activities of different parts in peanut gynophores and effects of PAO and POD on peanut gynophore entering into soil were studied by using six peanut varieties.The results showed that the different parts of peanut gynophores had different PAO and POD activities which were the highest in the peak parts,the higher in the middle and the lowest in the upper;PAO and POD activities of six peanut varieties were different and days of gynophore growth had few influences to PAO and POD activities.
ZHANG Qian-mei , CHEN Bei-guang , ZHOU Guo-yi
2006, 27(1):79-83. DOI: 10.7671/j.issn.1001-411X.2006.01.021
Abstract:应用9种计算方法,对鼎湖山自然保护区2种主要林型——针阔叶混交林和南亚热带常绿阔叶林的种间联结关系进行了分析.结果表明:先用方差比率法(VR)验证整个群落的总体关联性,再以x^2统计量来确定物种间联结性,然后与种间联结系数(AC)和成对物种间匹配系数(OI)等方法共同分析南亚热带森林的种间关系效果会较好.南亚热带常绿阔叶林研究的结果比针阔叶混交林更精确.对种间联结系数AC出现+1、-1的情形,把a、b、c、d为0的都加权为1后的结果,便于在2个林型间进行比较,更能体现出种间关系的动态变化和延续性,而不是种间关系的突变.
LIN Wei-qiang , JIA Xiao-rong , CHEN Bei-guang , XIE Zheng-sheng
2006, 27(1):84-87. DOI: 10.7671/j.issn.1001-411X.2006.01.022
Abstract:Two common formulas(Shannon-Wiener index and the reciprocal of Simpson index) were used to calculate the niche breadths among 15 populations of the arborous layer of the secondary forest community in Maofengshan.The niche breadths of most populations were narrow,while few populations were wide,which reflected a simpler community structure.The niche overlaps of 15 main populations were calculated using the niche similarity proportion,which revealed the inter-population niche overlaps and similarities using resources were not higher and the secondary community stayed in a stage of succession.The relationship between the niche overlap and breadth was not obviously correlated.
FENG Cheng-hao , WU Hong , ZHAO Sheng
2006, 27(1):88-91,116. DOI: 10.7671/j.issn.1001-411X.2006.01.023
Abstract:There were flourishing trichomes on the epidermis of Pogostemon cablin's stem and leaf,and they also have distinct difference in shape and development stage.According to the shape of head,the glandular hairs were divided into capitate and peltate glandular hairs.They all occurred very early and their initial cells came from protoderm,which usually originated from the first and second primordium or 1-2 internode of stem.Their developmental process may be divided into four stages,i.e.primitive cell stage,basal cell stage,stalk cell stage and apical cell stage.The origination of non-glandular hairs was later than that of glandular hairs,but lasting origination time in the former was longer than that in the latter.Non-glandular hairs usually started to occur in the secondary immature leaf,and also continue to occur in mature leaves and stems.After initial cells formed,they began to periclinal division to develop into basal cell and head cell.Head cell also began to periclinal division(once or more) to develop into the composing cells of non-glandular hairs.
SHU Gang , GAO Ping , ZHU Xiao-tong , JIANG Qing-yan , WANG Zhong-gang , FU Wei-long
2006, 27(1):92-95. DOI: 10.7671/j.issn.1001-411X.2006.01.024
Abstract:试验选用1日龄优质粤黄鸡360只,随机分为4组,每组设3个重复,每个重复30只,通过饮水添加不同剂量(第Ⅰ组0,第Ⅱ组0.01mg/L,第Ⅲ组0.05mg/L和第Ⅳ组0.10mg/L)甘氨酰谷氨酰胺(glyeyl-glutamine,Gly-Gin),以观察其对粤黄鸡肉品品质的影响,试验期91d.试验结果表明:(1)0.10mg/L的甘氨酰谷氨酰胺处理组胸肌剪切力较对照组有显著提高(P〈0.05);但对腿肌剪切力无显著影响;(2)0.10mg/L的甘氨酰谷氨酰胺处理组胸肌肉色色度较对照组有一定增高的趋势,但差异不显著(P=0.059);(3)0.10mg/L的甘氨酰谷氨酰胺处理组胸肌和腿肌的终pH值显著高于对照组(P〈0.05);(4)0.05mg/L的甘氨酰谷氨酰胺处理组胸肌初水分含量显著高于对照组(P〈0.05);(5)所有剂量甘氨酰谷氨酰胺对肌肉失水率以及肌肉中粗蛋白、粗脂肪含量均无显著性影响.以上结果提示,甘氨酰谷氨酰胺能够降低肌肉嫩度,增加肌肉的保水性能,改善肉色.
CHEN Wei-zhou , LI Yuan-you , SUN Ze-wei , HUANG Jun-hui , DU Yong-bing , TAN Yan
2006, 27(1):96-100. DOI: 10.7671/j.issn.1001-411X.2006.01.025
Abstract:以n-3高度不饱和脂肪酸(n-3HUFA)含量(w)为1.12%、2.40%、3.70%、5.85%的4种配合饲料或冰鲜杂鱼喂养花尾胡椒鲷Plectorhynchus cinctus雌亲鱼1周年,研究饲料中n-3 HUFA含量对性腺、肌肉和肝胰脏等组织的脂质含量和脂肪酸构成的影响.结果显示,各组织中的脂质含量在各投喂组之间无显著差异(P〉0.05).然而,在1.12%、2.40%和3.70%(w)投喂组,其性腺和肌肉脂质中18:2n-6、二十碳五烯酸(EPA)、n-3多不饱和脂肪酸(n-3 PUFA)、n-3 HUFA的含量与饲料中相应脂肪酸的含量存在较明显的平行变化趋势;在性腺脂质中,1.12%、2.40%、5.85%(w)投喂组的二十二碳六烯酸(DHA)和1.12%(w)投喂组的n-3 PUFA、n-3 HUFA的含量都显著低于冰鲜杂鱼投喂组(P〈0.05);在1.12%、2.40%和3.70%(w)投喂组,上述3种组织中的n-3 PUFA、n-3 HUFA、DHA含量以及DHA/EPA比值都显著高于饲料中相应脂肪酸的含量和比值(P〈0.05).结果表明,花尾胡椒鲷组织中的脂肪酸构成受饲料中n-3 HUFA含量影响,受影响的顺序是性腺〉肌肉〉肝胰脏;组织对n-3 PUFA、特别是DHA具有较强的选择性保留作用,说明这些脂肪酸在性腺发育成熟中具有重要的生理作用.
LUO Qing-fang , LIU Hua-yuan , GUO Xiao-feng
2006, 27(1):101-103. DOI: 10.7671/j.issn.1001-411X.2006.01.026
Abstract:Based on the reported sequences of NA gene of avian influenza virus(AIV) H9N2 subtype,a pair of primers was designed for the amplification of NA gene from a strain of AIV H9N2 subtype isolated from a pheasant(A/Pheasant /Guangdong /GZI/2003(H9N2) by RT-PCR.Sequence analysis indicated that the NA gene was 1 458 bp and the open reading frame(ORF) was 1 401 bp in length,encoding for 466 amino acids,sharing more than 98% identities with A/Chicken/Guangdong/11/97(H9N2).
2006, 27(1):104-106. DOI: 10.7671/j.issn.1001-411X.2006.01.027
Abstract:为了从分子水平研究狂犬病糖蛋白基因(G)从第4位重排至第1位后病毒的结构、功能及病毒致病性的变化,运用基因突变、反向遗传技术的原理与方法,将狂犬病毒(RV)糖蛋白基因从野生型的第4位(N-P-M-G-L)重排于第1位(G-N-P-M-L),并成功地拯救已发生基因重排的狂犬病毒.
WU Chang-li , ZHANG Shou-quan , GAO Shu-jing , JIANG Qing-yan , FENG Ding-yuan
2006, 27(1):107-109. DOI: 10.7671/j.issn.1001-411X.2006.01.028
Abstract:在TYH受精液中添加促卵泡素(FSH)和苯甲酸雌二醇(E2),检测不同浓度的FSH和E2对昆明小鼠的体外受精率的影响;检测在M16、CZB两种培养液中添加胎牛血清(FCS)与否对昆明小鼠早期胚胎体外发育的影响.结果表明:在.rYH中添加1.0IU/mL FSH,受精率和2-细胞胚率显著高于对照组;在TYH中添加0.1μg/mL E2,受精率和2-细胞胚率也显著高于对照组.在CZB中添加体积分数为10%FCS,可显著减少2一细胞期体外发育阻断,并显著提高小鼠胚胎体外发育的囊胚率,可见,以TYH添加1.0IU/mL FSH和0.1μg/mL E2为受精液,以含体积分数为10%FCS的CZB为胚胎培养液,小鼠胚胎可获得较高的囊胚率.葡萄糖并不是小鼠胚胎形成囊胚所必需的.
2006, 27(1):110-112. DOI: 10.7671/j.issn.1001-411X.2006.01.029
Abstract:在室内条件下采用浸渍法分别测定了4种杀虫剂的田间常用最低浓度对梭毒隐翅虫Paederus fuscipes成虫死亡率和捕食功能的影响.结果表明,梭毒隐翅虫对W=10%高效杀虫死水剂最敏感,对W=22%蚜虱灵可湿性粉剂、W=40%氧乐果乳油水剂次之,3种药剂处理24h后,天敌种群的功能减退率依次为93.293%、84.288%和75,848%.而W=18%杀虫双水剂对它的致死率及捕食功能减退率影响分别为(3.334-3.33)%和19.108%,相对其他3种药剂都较小.4种杀虫剂对残存个体捕食功能的影响均超过其致死作用.
PANG Yan-jun , RONG Xin , SHI Li-li
2006, 27(1):113-116. DOI: 10.7671/j.issn.1001-411X.2006.01.030
Abstract:在100 mmol/L NaC l胁迫下,经蒸馏水吸胀处理的LHN(耐盐)和IR26(盐敏感)水稻种子的发芽势、发芽率和发芽指数均较对照下降,LHN分别下降12.2%、12.5%和3.12,IR26分别下降46.6%、33.6%和9.42,IR26下降的幅度大于LHN.吸胀后,不同质量浓度(2、1、5×10-2、5×10-4和5×10-6mg/L)的外源茉莉酸甲酯(M e-Ja)对LHN和IR26在盐胁迫下发芽势、发芽率和发芽指数影响不同.以相对盐害率判断,质量浓度为2和5×10-6mg/L的Me-Ja对LHN在盐胁迫下萌发的促进作用不显著,质量浓度为5×10-2和5×10-4mg/L的M e-Ja表现为促进作用,质量浓度为5×10-2mg/L的M e-Ja促进作用最大;而对于IR26在盐胁迫下的萌发,5种质量浓度的M e-Ja的都具有显著的促进作用,且质量浓度为5×10-4mg/L的促进作用最大.
KANG Jie , HUANG Han-quan , CHEN Kao-ke , FU Mian-xing , CUI Da-fang
2006, 27(1):117-120. DOI: 10.7671/j.issn.1001-411X.2006.01.032
Abstract:Based on the research of plant community in Bijiashan Park of Shenzhen City,vegetation landscape could be divided into five major types: evergreen broad-leaved forest,evergreen coniferous forest,evergreen coniferous and broad-leaved mixed forest,meadows of sparse trees and orchard.Based on the observation of representative plant of landscape in Bijiashan Park,plant phenologies was noted and confirmed,and vegetation landscape effect was studied.The result showed that the vegetation landscape diversity of the whole park was low.It was suggested that more local trees should be planted there to increase the vegetation landscape diversity.
TAN Guan-zhu , FENG Zhi-jian , HE Zhong-jian , ZHENG Zhou-xiang , ZHAO Jun-jing
2006, 27(1):121-123. DOI: 10.7671/j.issn.1001-411X.2006.01.033
Abstract:Brainea insignis is one of the fern species belonging to the second class national key protected plants.According to the investigations in 16 sample plots totaling(1 600 m~2) in Yinpingzui Nature Reserve of Dongguan City,the communities consist of 99 species,88 genera,60 families of vascular plants.Analysis of floristic geographical elements showed that the percentage of tropical elements is high,accounting for 83.95% of the total numbers of genera.The community can be structurally divided into arborous layer,shrub layer and herbaceous layer.In the arborous layer Itea chinensis,with an importance value of 35.05%,is the biggest,and in the shrub layer the biggest one is Brainea insignis with an IV of(38.61%).It was suggested that Brainea insignis played an importanst role in the community.
2006, 27(1):124-124. DOI: 10.7671/j.issn.1001-411X.2006.01.034
Abstract:Two new recorded species of Boraginaceae:Bothriospermum chinense Bge.,Cynoglossum amabile Stapf et Drumm.from Guangdong Province are reported with their distributions and medicinal values.All specimens examined are preserved in Herbarium of South China Botanical Garden,the Chinese Academy of Sciences(IBSC).
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