Abstract:以2个常规香稻品种和1个杂交香稻组合为材料,通过人工气候室控温的方法,研究了不同温度处理对香稻产量和品质的影响.结果表明:灌浆结实期不同温度处理以日均温23℃香稻的产量最高、米质最好、香味得分也最高;高温处理(日均温30℃)使香稻的结实率、实粒数显著降低,虽然千粒质量稍有增加,但最终表现为产量显著降低;高温处理显著降低了精米率、整精米率、直链淀粉含量、碱消值、食味得分和香味得分,而使垩白度增加,胶稠度变硬,从而对香稻品质带来显著影响.

Abstract:研究了采前使用不套袋、白色纱网袋、黑色遮阴网、绿色纱网袋、蓝色纱网袋(以下分别称为对照、白袋、黑袋、绿袋、蓝袋)5种处理对‘石硖’龙眼果实品质和贮藏生理的影响.结果表明:5种处理间落果率无显著差异;套袋对果实大小和单果质量影响不一;套袋处理可以显著改善果实的外观颜色,提高其亮度(L*值),增加其色饱和度(C*值),且与对照差异极显著;提高果皮中类黄酮(对照、白袋、黑袋、绿袋、蓝袋分别为2.16、2.67**、2.67**、2.57*、2.60*U.g-1)和花青苷的含量,促进果皮叶绿素的降解,提高果实中维生素C含量(对照、白袋、黑袋、绿袋、蓝袋分别为0.56、0.64*、0.59、0.68**、0.61 mg/g),但对可溶性固形物和可滴定酸含量影响不一;各套袋处理均促进了龙眼4℃贮藏过程中果皮的褐变,改变了龙眼果实在4℃贮藏过程中的生理变化,表现为提高了呼吸强度,促进果皮细胞膜透性的升高,也提高了PPO酶和POD酶活性.

Abstract:利用BIOLOG ECO微孔板分析施人不同堆肥后，引起土壤微生物群落防治土传病害功能变化的碳源，结合3种数学分析方法对移栽后13、33、55d的土壤进行分析，共选出10种特征碳源，分别是吐温80、D-木糖、苯乙基胺、腐胺、α-D-乳糖、β-甲基-D-葡萄糖苷、γ-羟基丁酸、α-丁酮酸、L-苯基丙氨酸和L-苏氨酸．利用31种特征碳源作主成分分析图，结果表明：PCA图中各处理点的分布情况较相似，能较好地反映发病情况，且特征碳源能更敏感地反映土壤微生物群落特征的变化．聚类分析结果表明，与31种碳源相比，利用特征碳源的聚类图能更合理地将各处理按发病情况归类．

Abstract:The four-armed olfactometer was used to measure the olfactory response of Trichogrammatoidea bactrae Nagaraja,Trichogramma confusum Viggiani,T.raoi Nagaraja and T.closlerae Pang et Chen to the volatiles derived from eggs,hexane extract of egg surface and ventral squama of Corcyra cephalomica(Stainton) in laboratory.The results showed that adults of these four species of trichogrammatid prolonged their retention time in the treated areas,and indicated the kairomones from C.cephalomica could stimulate an intensive search behavior of adults.

Abstract:采用环境扫描电镜观察中华微刺盲蝽Campylomma chinensis Schuh的触角，结果表明，中华微刺盲蝽触角由基节、柄节、梗节和鞭节组成．绝大部分触角感器位于触角的背面、腹面和外侧面．早、6两性触角上均存在7种感器，即毛形感器、刺形感器、锥形感器、腔锥形感器、具弯钩形感器、蒲姆氏鬃毛和腔形感器，各种感器在早、6两性触角上的分布和数量没有明显的区别．此外6性触角还有3种感器，即乳形感器、钟形感器和圆柱形感器，其功能可能与接受性信息素有关，其中乳形感器是一种新发现的昆虫触角感器．

Abstract:对桔小实蝇Bactrocera dorsalisHendel在中国的适生性进行了研究,结果表明,该虫的世代发育起点温度和有效积温分别为10.675 7℃、501.724 0日度.根据桔小实蝇的致死温度、有效积温和生物气候相似距,构建了桔小实蝇适生性分析计算机模型.用中国670个气象站30年的气候资料运行该模型,将桔小实蝇在中国的定殖风险区划分为高度危险区、危险区、轻度危险区和安全区,分布北界为(30±2)°N,适生区面积占全国的31.64%,1年发生3~11代,以4~8代为主.

Abstract:Comparison of culture of Meloidogyne spp. in varieties of Lycopersicum esculentum and Solanum tuberosum was conducted. It was showed that four varieties of potato could successfully culture the M. incognita, and that the reproduction of the nematode in variety of Daxiyan and 958 were significantly better than in other two varieties. M. incognita, M. javanica and M. arenaria could be successfully cultured on variety of 958, but the best for M. incognita.

Abstract:There is a diverse and peculiar biodiversity of invertebrates which play a vital role in maintaining native bird populations in New Zealand.The current biodiversity and threatened species of invertebrates in New Zealand were briefly reviewed in the present paper.There are 31 species of invertebrates included in the list of the most threatened invertebrates(18 species for insects,1 species for mite,1 species for annelid and 11 speices for molluscs).The main threats of invertebrates facing today are human activities,destroy of habitats and invasive alien species.

Abstract:The male of Coptotettix fangchengensis Zheng et Jiang was discoveried and described, and its specimens were kept in Department of Chemistry and Biology, Hechi University.

Abstract:用经磷酸缓冲液处理后的多孔陶瓷膜作为固定化酶的载体，采用物理吸附法固定木瓜蛋白酶．试验结果表明，当溶液酶质量浓度为1．0～2．0mg／mL、pH7．0、固定2h时，固定化酶活力最高达111．1U／g，活力回收达57．9％．固定化酶反应最适pH为6．0～8．0，最适温度为60～70℃，70℃下保温4h活力仍保持在50％以上，半衰期54d．试验证明，多孔陶瓷膜吸附能力强、吸附速度快，制备的固定化木瓜蛋白酶的pH稳定性、热稳定性、贮藏稳定性明显优于溶液酶．

Abstract:从广东酸性土壤分离得到一种编号为2-1A1的丛枝菌根真菌.其孢子在土壤中单生,未见孢子果,椭圆形,黄色至褐色,孢子较大(170μm×230μm),孢壁一层,厚约11μm,M elzer s反应为黄色.其形态特征与Glomus flavispo-rum的特征描述相一致.提取其单孢子DNA,Nested PCR扩增其18S rDNA的NS31-AM1区,经序列测定,并在Gene-Bank上比对,进行系统进化树分析,结果表明,2-1A1与Glomussp.(DQ371674)的亲缘关系最近,序列同源性为98%.分子鉴定的方法只能将其鉴定到球囊霉属Glomus.

Abstract:利用叶绿素荧光技术测定了银杏不同品种叶绿素荧光参数．结果表明：泰兴一号的PSⅡ最大光化学效率（Fv／Fmax）、PSⅡ电子传递量子效率（ФPSⅡ）、表观光合电子传递速率（ETR）及光化学猝灭系数（qP）均高于顺德清晖园银杏，而非光化学猝灭系数（qM）较低．可见，泰兴一号生长较快，具有潜在高生物产量的生理生化基础．不同品种银杏光响应曲线、ETR对光强度响应的初始斜率以及光合色素的含量进一步证实了以上论点．

Abstract:A research on bat diversity has been done in Guangzhou City.1 400 bats were observed and 91 specimen were collected which belong to 12 species.In addition 3 species were recorded by interview,thus there are 15 species of bats(belonging to 4 families,9 genera) in total in Guangzhou.In which Cynopterus sphinx and Tylonycteris pachypus are listed in the China Red Data Book of Endangered Animals.Our findings showed that 12 species belong to the Oriental type(80% of the total),3 species belong to the Palaearctic type, the Monsoonal type and the South China type respectively(20% of the total).Bats in Guangzhou City have 4 habitat types:Rhinolophus,Hipposideros and Rousettus leschenaulti live in a cave,Cynopterus sphinx and Tylonycteris pachypus,etc,live in a tree,Nyctalus velutinus and Pipistrellus,etc,live in a house,Scotophilus kuhlii live in a tree or a house.Bats play an important ecological roles in the eco-system in Guangzhou City.Entomophagous bats prey on pests such as gnat and miller,etc,they are one of the valid natural enemies of pests.Fruit bats may eat fruit such as litchi,longan and banana,etc.From the questionnaire,75.5% fruit farmer ever suffered from the fruit bat(annually.)

Abstract:Niche characters of the dominant plants and the feeding plants for Hainan black-crested gibbons, Nomascus hainanus in Hainan Bawangling National Nature Reserve were studied by plot survey to understand their adaptations to the environments and competitions. A total of 190 species were recorded in the plot with an area of 4 800 m2, of which 41 species, accounting for 21.58% and 58.89% in species number and importance value, respectively, were fed by the gibbons. There were only 24 plants or 12.63% of the plants with a niche breadth higher than 1, while other 166 plants or 87.36% of the plants with a niche breadth lower than 1. Among the feeding plants, there were only 13 plants or 31.71% of them with a niche breadth higher than 1, while other 28 plants or 68.29% of them with a niche breadth lower than 1. And among 17 species pairs with an overlap index higher than 0. 5, 12 pairs are both feeding plants. This study indicates that the current habitat of montane forest is less suitable for the feeding plants so to recover the lowland rain forest are suggested to carried out as soon as possible.

Abstract:By applying the method of "spatial sequence as a surrogate for temporal change",size structure of Schima superba population was analyzed with diameter as the indices of individual size.The results showed that in the early period after S.superba population invaded the forest of Pinus massoniana or Liquidamber formosana,size structure of the population was characterized by a growing type(growing,stable and senescent form).And in the processes of succession,the density sequences of S.superba population declined.Through analyzing dynamics of S.superba population,it showed that S.superba population had the characteristic of climax pioneer species, which was different from not only pioneer species such as P.massoniana,and shade-intolerant tree species such as L.formosana,but shade-tolerant tree species such as the Cinnamomum and the Castanopsis.S.superba tree were in the middle position of the forest succession stages.

Abstract:Nine ferns are reported as new records from Hainan Island,China.They are Allantodia alata(Christ) Ching,Allantodia petelotii(Tard.-Blot) Ching,Microsorum steerei(Harr.) Ching,Pteris decrescens Christ,Pteris majestica Ching ex Ching et S.H.Wu,Pteris oshimensis Hieron,Selaginella boninensis Bak.,Cyrtomium fortunei J.Sm.and Polystichum kwangtungense Ching.All the voucher specimens for these ferns in the present paper are preserved in Herbarium of South China Botanical Garden,the Chinese Academy of Sciences (IBSC).

Abstract:Test of Xinfeng sub-population of the main growth breeding population of Pinus taeda was assessed for traits. Selection was carried out using tree volume. The results showed that genetic gains of combining selection were higher than that of family selection. Giving the selection rate as 4/100, then the genetic gains for combined selection and family selection were 48.6% and 21.8% respectively. For 25/100 of selection rate, the genetic gains were 30.3% and 11.1% respectively. Possibility of at the age of six years, aimed at improvement for productive deployment and establishment of selection breeding population, was discussed. It raised selection for the establishment of the next generation breeding population that could be realized only when most trees in the test stand of sub-population reach the age of fruiting.

Abstract:Biomass allometry of Pterocarpus indicus seedlings under different fertilization was studied based on the multiple regression analysis and analysis of covariation(ANCOVA).Combined with residual analysis,the biomass estimation model was established.The effects of fertilization on biomass distribution were also analyzed based on the Duncan's multiple comparison.The result showed that: 1) there were no effects of the fertilization on the biomass allometry of Pterocarpus indicus seedlings,2) the biomass distributions were affected by the fertilization,3) there was inverse relationship between the leaf biomass distribution and the root biomass distribution,i.e.the biomass allocation to leaf increased while the biomass allocation to root reduced.

Abstract:根据家鸡催乳素受体基因胞外域序列（GenBank号：D13154）设计并合成1对覆盖编码区58～1249位核苷酸的引物，以家鸡垂体总RNA为模板，经反转录-聚合酶链反应（RT-PCR）方法获得了特异性片断，将该片断克隆入pMD18-T载体并转化感受态细菌Ecoli DH5α永久保存．通过设计第2对引物扩增PRLR胞外域cDNA片段，该片段经酶切后克隆到表达载体pRSETA的EcoRⅠ和HindⅢ两酶切位点之间，构建重组质粒pR-PRLR．该质粒在转化感受态细菌E．coli B121（DF．3）后，在IPTG诱导下促使重组菌表达了相对分子质量为53640左右的重组融合蛋白，表达量在0．1mmol／L IPTG诱导5h时达到最高，约占总菌体蛋白的17．6％左右．表达产物可经体积分数为50％的Ni-NTA凝胶纯化，说明重组蛋白中具有正确的His-tag标签及之后的鸡催乳素受体编码区胞外域序列．

Abstract:To study the epidemiology of pseudorabies(PR),the investigation of temporal and spatial distribution of pseudorabies(PR)were conducted in Guangdong Province from 1998 to 2004.The seropositive rates of PR were higher(>20%) in Dongguan,Huizhou,Shenzhen,Yangjiang,Zhuhai,and lower(<10%) in Qingyuan and Shantou.The highest was 3.82 times as much as the lowest.The seropositive rates of 51.7% pig farms were less than 10%.In temporal distribution,a peak of PR seroprevalence may appear every 5 months.Forecast by ARIMA model revealed that seropositive rate in Jan 2005 may be 14.23% approaching the true value 15.06%.Analysis of spatial-temporal correspondence showed that the high seroprevalence of PR for Pearl River Delta appeared in 2002 and 2003,for the north in 2001.In 2002 and 2003,the low seroprevalence of PR were observed in the east.Two-dimension chart covered 83.3% original information.

Abstract:The amantadine-resistance of an H5 subtype avian influenza virus(AIV) isolate was characterized.The M gene of AIV 178 isolate was obtained by RT-PCR,and sequence analysis showed that M2 protein contained S31N substitution.Animal trial revealed that the isolate was resistant to the treatment of 200 mg/L amantadine via drinking water.The results suggested that AIV 178 isolate was amantadine resistant,and the position 31 amino acid point mutation in M2 protein may confer the resistance.

Abstract:为了研究鸡γ-干扰素（ChIFN-γ）基因的功能并进一步探究其在控制家禽传染病方面的应用，根据GenBank已发表的ChIFN-γ cDNA基因序列自行设计引物，应用反转录-聚合酶链式反应（RT-PCR）技术从伴刀豆球蛋白A（ConA）刺激培养鸡脾淋巴细胞中提取的总RNA中扩增得到ChIFN-γ，然后连接到pMD18-T载体上并转化到Top10感受态细胞中，获得阳性重组质粒并进行正确性序列测序．核甘酸序列结果表明，试验得到了长为513bp的ChIFN-γ基因，与原鸡（Gullus）IFN-γ的同源性可高达100％，其含有1个495bp的开放性阅读框架，编码164个氨基酸，推测其成熟蛋白的相对分子质量约为18000．

Abstract:采用RT-PCR方法从猪肾脏皮质组织中扩增出pEGF基因，将其连接到克隆载体pMD18-T上，经测序鉴定正确后，再克隆到原核表达载体pET-41a（＋）上，构建重组表达质粒pET-EGF．用重组质粒转化Ecoli BL21（DE3）宿主菌，经IPTG诱导表达后利用SDS-PAGE电泳和Western-blotting对GST—EGF融合蛋白进行分析，结果表明37℃诱导表达的融合蛋白含量较高，占菌体总蛋白的20．2％；30℃诱导表达的可溶性融合蛋白含量较高，占菌体总蛋白的7．7％．

Abstract:The relationships between the ground water rise and the water consumption per bank length, the ground water depth and the improvied ecological area, were studied by using observied data of 5 time input water to Talimu River, dynamic planning theory and great system optimization theory. The mathematics mode of input water on Talimu River was contructed and water allocation in different research area was computed.

Abstract:Based on analysis of the information management status, goal and functions of the sugarcane mechanization production system, a information management platform was constructed for sugarcane mechanization production system. The detail elements and functions of every sub-platform and database were clarified. Some integrated information management scenarios were provided for sugarcane modernization production.

Abstract:The simplify criterions for construction of multi-resolution models was discussed based on geographic features. For the problem about carck brought out in model triangularization, a new technique combining quad-tree has been invented.

Abstract:利用离子色谱仪同时测定降水中的3种有机酸根离子和4种无机阴离子，通过对分析条件的优化，确定了最佳的色谱条件：以4．0mmol／L Na2CO3与1．0mmol／L NaHCO3为流动相，流速0．8mL／min作等度洗脱，在10min内实现基线分离并完成电导检测．7种组分的检测限在0．5～20．0nmol／L之间，线性范围跨越2个数量级以上．该方法用于雨水样品的分析，结果表明：平均加标回收率为92．1％～106．2％，相对标准偏差0．30％～4．03％.

Abstract:以电化学沉积法将Pt纳米颗粒沉积在碳纳米管(CNTs)上,并用扫描电镜(SEM)和能量色散X射线谱(EDS)对Pt/CNTs进行了表征.循环伏安图表明Pt/CNTs电极对L-半胱氨酸的电催化效果比石墨、Pt/石墨要好.同时,计时电流曲线表明Pt/CNTs电极对L-半胱氨酸在0.5~100.0μmol.L-1范围内有线性响应,其检测限为0.3μmol.L-1.

Abstract:Effects of hormone on shoot formation and plant regeneration were studied in reproduction of cotyledon as explant in Brassica alboglabra Bailey.The results showed that shoot formation frequency from the cotyledons with petiole was higher than that of the cotyledon segments without petiole.The maximal shoots formation frequency was 93.75%,When cultured on MS medium containing 0.05 mg/L NAA,2.0 mg/L 6-BA,30.00 g/L sucrose and 8.00 g/L agar(pH 5.8).The elongated shoots rooted in MS medium without hormone or MS medium containing 0.1 mg/L NAA,with 100% and 86.11% of rooting rates,respectively.More and stronger roots were induced in the latter medium.

Abstract:The life table and interference index of population control(IIPC) were used to evaluate the effect of petroleum spray oils on the natural population of Chloropuicinaria psidii Mask by observation in the laboratory and the systematic investigation in the field.The result showed that the trend index of population(I) of C.psidii was 7.96.The trend index of population in treatment of petroleum spray oils was 0.237,and its IIPC was 0.030.It was indicated that the petroleum spray oils had good control effect of 97% on C.psidii.

Abstract:six to eight-week-old BALB/c mice and guinea pigs were immunized with eukaryotic plasmids and normal FMDV type O vaccine by intramuscular injection, respectively for three times, and immunization interval was two weeks. The F fragment codes for the amino acids at sites 140-160 and 200-213, the major antigenic epitopes of FMDV type O. The immune effects of DNA vaccines were compared with that of conventional vaccine at MTT, T lymphcyte subgroup, neutralizing antibody, resistance to FMDV and histopathology. The results showed that DNA vaccines pcDNA-VPI and pcDNA-F could induce humoral and cellular immunity. BALB/c mice and guinea pigs immuned with pcDNA-F could were resistant to FMDV, but there were difference with conventional vaccine.

Abstract:将生物质棕榈油在一定条件下与偶氮二乙丁腈（AIBN）、活性单体甲基丙烯酸缩水甘油酯（GMA）和甲基丙烯酸羟乙酯（HEMA）共同作用，转化为聚合物材料．对该聚合物材料的结构用红外光谱进行了表征，热行为用热重及差热分析进行了研究，结果表明，聚合物材料在180℃下稳定性良好．

Abstract:The traditional method for dynamic life table need to trace the whole life process of a population and is difficult to apply to long-lived plant species research. The paper devises a method using data from two surreys for making dynamic life table of plant species to improve the efficiency of research.

Abstract:Sponsored by South China Agricultural University, Journal of South China Agricultural University publishes a wide range of papers in Agricultural Sciences. Featuring in the Plant Protection Section for publishing regular articles, Journal of South China Agricultural University maintains a leading position in publishing papers in agricultural sciences in China. It is one of China＇s core journals in comprehensive agricultural sciences and in agricultural plant protection, and also the award - winning journal for many times. It is abstracted and indexed in more than 30 digest journals and databases.