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  • Volume 31,Issue 3,2010 Table of Contents
  • Analysis of Dominant Species of Microbial Community in Heavy Metals Contaminated Soil from Dabaoshan Area

    GUO Jian-hua ,  LI Hua-ping ,  ZHU Hong-hui

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.014

    Abstract (1353) HTML (0) PDF 467.12 K (1998) Comment (0) Favorites

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  • Effects of Xylooligosaccharide on Growth and Activity of Digestive Enzyme in Litopenaeus vannamei

    HUANG Yan-hua ,  WANG Guo-xia ,  LIU Xiang-he ,  ZHOU Ye ,  HUANG Wen-qing

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.015

    Abstract (1264) HTML (0) PDF 343.82 K (1594) Comment (0) Favorites

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  • Cultivable Gut Microbiota of Juvenile and Early Young Grouper Epinephelus coioides

    SUN Yun-zhang ,  LING Ze-chun ,  YANG Hong-ling ,  YE Ji-dan ,  LIN Wen-yan

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.016

    Abstract (1282) HTML (0) PDF 469.90 K (1843) Comment (0) Favorites

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  • Analysis of Nutrient Components and Evaluation of Nutritive Quality in the Muscle of Oplegnathus fasciatus

    HU Ling-ling ,  LI Jia-er ,  OU You-jun ,  CAI Wen-chao

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.017

    Abstract (1272) HTML (0) PDF 384.82 K (1444) Comment (0) Favorites

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  • Effect of Somatostatin on High Fat Diet-Induced Obesity and Oxidative Stress

    LI Wu ,  SHI Yong-hui ,  CUI Jue ,  XIAO Ying ,  LE Guo-wei

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.018

    Abstract (1096) HTML (0) PDF 392.13 K (1463) Comment (0) Favorites

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  • Studies on Rat Bladder Stone Model Caused by Chemical Pure Reagent of Melamine

    CHEN Jia ,  ZOU Yi-hai ,  ZHANG Wei ,  CHEN Yuan ,  ZHANG Yong-bin ,  JIN Peng-fei ,  SUN Jing-chen

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.019

    Abstract (1114) HTML (0) PDF 297.82 K (1512) Comment (0) Favorites

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  • Expression of Mouse Manganese Superoxide Dismutase in Escherichia coli and Its Purification

    WANG Feng ,  HUANG Lu-yuan

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.020

    Abstract (1169) HTML (0) PDF 361.78 K (1469) Comment (0) Favorites

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  • Analysis on mtDNA D-loop HVR-I Polymorphism of the Chinese Sharpei

    LIU Qing-shen ,  SU Yan-hua ,  FENG Ding-yuan

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.021

    Abstract (1093) HTML (0) PDF 338.98 K (1451) Comment (0) Favorites

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  • Experiments of Lifting Process for the Lodged Sugarcane

    MOU Xiang-wei ,  XIE Fu-xiang ,  OU Ying-gang ,  GONG Jian-hua

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.022

    Abstract (1066) HTML (0) PDF 294.30 K (1535) Comment (0) Favorites

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  • The Application on the Sugar Cane Forecast with the Neural Networks Using Improved Genetic Algorithms

    XU Yong-chun ,  ZHANG Sen-wen

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.023

    Abstract (1138) HTML (0) PDF 220.61 K (1430) Comment (0) Favorites

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  • An Improved Method for Determining the Density of Increment Core Wood Samples Using Hydrostatic Weighing Techniques

    HU Chuan-shuang ,  SU Zhi-yao ,  YUN Hong ,  WEN Wei ,  WANG Ting ,  LU Qun-xia

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.024

    Abstract (1586) HTML (0) PDF 294.60 K (2025) Comment (0) Favorites

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  • Fuzzy Synthesis Evaluation Methods on the Performance of Automobile Engine

    MAO Cai-yun ,  WU Mu-chun ,  LU Hua-zhong

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.025

    Abstract (1115) HTML (0) PDF 255.63 K (1525) Comment (0) Favorites

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  • Spermatophyte Resources of Yinggeling Mountain,Hainan

    ZHANG Rong-jing ,  CHEN Hong-feng ,  YE Yu-shi ,  NG Sai-chit ,  XING Fu-xu ,  WANG Fa-guo

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.026

    Abstract (1204) HTML (0) PDF 228.61 K (1341) Comment (0) Favorites

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  • Screening Biocontrol Bacteria to Vegetable Root-Knot Nematodes

    HUANG Jin-ling ,  LIU Ji-shuang ,  LIU Zhi-ming ,  LU Xiu-hong ,  PAN Ping-hong ,  LU Guang-yi ,  QIN Bi-xia ,  LI Guo-dong

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.027

    Abstract (1184) HTML (0) PDF 235.88 K (1536) Comment (0) Favorites

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  • First Record of the Tortricid Genus Vellonifer Razowski(Lepidoptera)from China

    FANG Yi ,  CHEN Liu-sheng ,  WANG Min

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.028

    Abstract (1150) HTML (0) PDF 157.10 K (1706) Comment (0) Favorites

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  • New Records of Ferns from Guangdong Province,China

    ZHOU Jin-song ,  YAN Yue-hong ,  HE Zu-xia ,  XING Fu-wu

    2010, 31(3). DOI: 10.7671/j.issn.1001-411X.2010.03.029

    Abstract (1166) HTML (0) PDF 126.06 K (1592) Comment (0) Favorites

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  • Study on Photodegradation of Fenamiphos in Surface Soil

    WANG Dong ,  WANG Jing-guo ,  MU Kang-guo

    2010, 31(3):36-39. DOI: 10.7671/j.issn.1001-411X.2010.03.030

    Abstract (882) HTML (0) PDF 320.00 K (1530) Comment (0) Favorites

    Abstract:以太阳为光源研究了TiO2量、苯线磷初始质量比、土壤厚度对苯线磷在土壤中光解行为的影响.结果表明:苯线磷的光解符合准一级动力学方程,且随添加TiO2用量从0mg·kg-1增加到200mg·kg-1,苯线磷的一级光解动力学常数也增大,其中最快的降解速率是最慢的1.51倍.在含有TiO2的土壤中,添加质量比相对较低的情况下,苯线磷的降解较快;苯线磷在不同厚度土壤中的光解速率不同,土壤厚度较大的情况下,苯线磷的半衰期也比较大.

  • Studies on the Separation and Determination of Roxarsone and Multi Valence As in the Excreta of Chicken

    ZENG Jing ,  ZHUO Jia-zhen ,  CHEN Zhang-liu ,  SUN Yong-xue

    2010, 31(3):94-97. DOI: 10.7671/j.issn.1001-411X.2010.03.031

    Abstract (891) HTML (0) PDF 315.73 K (1270) Comment (0) Favorites

    Abstract:对鸡排泄物中洛克沙胂、总As及无机As(Ⅲ)和As(Ⅴ)的分离与测定进行了研究.采用磷酸氢二钾-甲醇混合液提取和载入固相萃取小柱净化,对洛克沙胂进行HPLC法分析,回收率≥74.4%,最低检测限为0.1μg/g.经微波消解处理后采用AFS法检测排泄物中总As含量,检出限为0.08μg/L,回收率≥94.7%.经磷酸二氢钾溶液振荡提取过滤后,依次经阳离子和阴离子交换树脂静态吸附与动态分离,采用AFS法分别测定As(Ⅲ)、As(Ⅴ)含量,检出限分别为0.084和0.210μg/L,回收率分别为87.8%～113.0%和81.1%～127.3%.

  • Prokaryotic Expression and Preparation of Polyclonal Antibody of Glycolate Oxidase Gene OsGLO1 from Rice

    ZHANG Jian-jun ,  XU Hua-wei ,  ZHOU Xiao-chui ,  WNAG Yu-qi ,  PENG Xin-xiang

    2010, 31(3):113-115. DOI: 10.7671/j.issn.1001-411X.2010.03.032

    Abstract (953) HTML (0) PDF 246.92 K (1481) Comment (0) Favorites

    Abstract:乙醇酸氧化酶是光呼吸代谢的关键酶.以水稻Oryza sativa叶片cDNA为模板,利用RT-PCR扩增水稻乙醇酸氧化酶基因(OsGLO1),并构建了该基因的原核表达载体pET23d-OsGLO1,转化E.coli BL21(DE3),经IPTG诱导表达融合蛋白.以纯化的OsGLO1融合蛋白为抗原免疫新西兰白兔,制备兔抗OsGLO1的多克隆抗体.Western-blot分析表明,制备的多克隆抗体能有效地检测水稻、菜心Brassica campestris、拟南芥Arabidopsis thaliana和菠菜Spinacia oleracea中乙醇酸氧化酶的表达,为进一步深入研究乙醇酸氧化酶在调控光呼吸及抗逆性等方面的作用奠定了基础.

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