Abstract:To explore a suitable mode of topping and the influence of upper leaves usability of flue-cured tobacco in Shixing, the northern Guangdong tobacco area, the effect of different topping modes (topping at buttoning stage, topping at budding stage, topping at early blooming stage)on the agronomic characters, major chemical composition changes of upper leaves, yield, quality and the quality of cured leaves of flue-cured tobacco was investigated. The results showed that the height of the plant, the effective leaf number and the length of inter-nodes increased significantly with a postponed topping stage, while the biggest upper leaf area and the girth growth decreased. Different topping modes made a big influence on starch, soluble sugar, nicotine for upper leaves stage, but not so obvious on total nitrogen. The output value of topping at early blooming stage and proportion of middle and superior tobacco leaves increased by 6.48% and 7.73%, respectively, compared with the common mode of topping (topping at budding stage), potassium content being 13.02%; starch and nicotine decreased by 15.68% and 5.99%, respectively, and sugar-nicotine ratio and soluble sugar-protein ratio increased slightly, and the inner elements being harmonious. In a word, topping at early blooming stage could improve tobacco upper leaves’ usability and was worth being promoted in production.

Abstract:Taking PH4CV×chang7-2 as the basic material, inbred lines were selected under the selective pressure of 6×10⁴, 9×10⁴, 1.2×10⁵ and 1.5×10⁵ plants/hm².The NCⅡdesign was composed of 4 backbone inbred lines and 5 representative lines which were selected from the F4 generation, and the yield performance, GCA and SCA were analyzed. The results indicated that GCA of 5 inbred lines selected under the 1.2×10⁵ plants/hm² were all positive, and the highest value 12F-3 was 12.74, the parents who had the highest value of SCA also came from the inbred lines under the selective density of 1.2×10⁵ plants/hm². Meanwhile，the yield of the combination which derived from that density was much higher than the best of CK，and the highest grain yield per plot was 15.57 kg from PH6WC×12F-5. The 1.2×10⁵ plants/hm² was considered the best density for PH4CV×Chang7-2 combination among the 4 selective densities.

Abstract:Major genes plus polygene mixed inheritance model and QTL IciMapping v2.2 Inclusive Composite Interval Mapping were used to study the genetic regulation of soybean fat content in F₂ segregating population materials of high protein Jiyu50 and high fat Jinong18 parents. The results showed that the soybean fat content trait complied with the polygenic inheritance model and was controlled by polygene. Polygene genetic rate was 79.15%. Two major effective QTLs and two micro effective QTLs on soybean fat content were detected and distributed in 12 (G), 17 (M) and 22 (F) 3 linkage groups, including a stable major QTL in two years.

Abstract:The genetic linkage map was constructed with 89 SSR markers based on a maize population consisting of 236 F₂ individuals from B15(not tender)×B20(tender). The linkage map covering 1 317.2 cM on a total of ten chromosomes with an average interval length of 14.8 cM could be used to locate QTL. The population of 236 F_2：3 families were evaluated for pericarp tenderness. With the method of composite interval mapping, four QTLs associated with pericarp tenderness were identified on the 3 ^rd, 4 ^rd, and 7^rd chromosomes, which explained 3.0%, 1.1%, 1.0% and 6.0% of phenotypic variance, respectively. The additive effects of 4 QTLs were all negative. The results revealed that all the QTLs had come from the parent with good pericarp tenderness, which could decrease pericarp tenderness and improve the tenderness of pericarp trait.

Abstract:Taking the Guangming New District in Shenzhen City as the study area, the spatial and temporal evolution of soil fertility properties were studied through principal component analysis of seven major indicators. The results showed that the main limiting factors of soil fertility had been changed into high bulk density and lack of nitrogen in 2011’s from low available phosphorus and potassium in 1984’s after 28 years’ evolution, but there were no significant differences in soil comprehensive fertility for the prominent fertility factors and limiting factors in the two periods were canceled out. Significant differences of soil integrated fertility and main limiting factors were observed in different areas, which could be caused by various planting models and different fertilization habits.

Abstract:Flow cytometry (FCM) was used to analysis the destruction of essential oils on membrane integrity of Spodoptera litura cultured cell line SL-1. The cytotoxicity of rotenone and capsaicin alone or in combination with essential oils against SL-1 cells were studied by MTT method. Flow cytometric analysis showed that the intra-cellular fluorescence intensity of accumulation in the membrane impermeable nucleic acid stain propidium iodide (PI) was promoted by 53.04% after 24 h treatment with 50 μg·mL^-1 of rue oil , followed by 34.23% and 33.67% after treatment with angelica oil and turpentine oil, respectively. After being treated with 100 μg·mL^-1 of patchouli oil, turpentine oil, rue oil, angelica oil, citronella oil, and ginger oil, the increase rates of the accumulation of PI were all higher than 40%. The values of median inhibitory concentration (IC₅₀) for rotenone and capsaicin against SL-1 cells for 24 h were 34.97 and 35.92 μg·mL^-1, respectively. When combined with 100 μg·mL^-1 of rue oil, the values of IC₅₀ were decreased to 12.69 and 13.26 μg·m^-1, and the other values were 14.56 and 11.392 μg·mL^-1 when combined with 100 μg·mL^-1 of turpentine oil, which were all lower than those treated with rotenone and capsaicin only. All these results indicated that rue oil and turpentine oil could obviously enhance the cytotoxicity with rotenone and capsaicin.

Abstract:Citrus canker is an critical bacterial disease in the world. Chemical control is one of the most important measures for the disease. In this study, the sensitivities to eight kinds of bactericides on 21 strains of Xanthomonas axonopodis pv. citri (Xac) mainly from Guangdong and Jiangxi Province were tested by using bacteriostatic experiment in the plate. The results showed that bismerthiazo (1 000×)， new phytomycin (2 800×) and oxytetracyclini hydrochloridum (1 300×) had few effects on 21 bacterial strains. Streptomycin, zhongshengmycin，peracetic acid，copper hy-droxide ziram and bouillie bordelaise had different levels of bacteriostatic action, while streptomycin had the best effect on Xac. However，some Xac strains showed different resistances to these fungicides. In addition, streptomycin and zhongshengmycin showed better control effects on citrus canker disease in the field.

Abstract:At the temperature of 26 ℃, effects of host age on the development and reproduction of Diachasmimorpha longicaudata(Ashmead), reared with the larvae of oriental fruit fly, Bctrocerat dorsalis（Hendal）, was investigated from the aspects of the parasitical efficiency，immature survival rate, wasp body size and sex ratio of its offspring, respectively.The results showed that host age had an obvious influence on the development and reproduction of D. longicaudata. Female wasps preferred to parasitize the 2^nd instar and the initial 3^rd instar larvae of oriental fruit fly, and the number of 63.00 and 85.50 host larvae parasitized at the corresponding ages were observed within 6 hours. But only 35.60 and 33.21 host larvae were parasitized when provided with the 1^st instar and the old 3^rd instar oriental fruit fly larvae, respectively. Development rate of D. longicaudata decreased with the increase of rearing host age. The generation development of D. longicaudata were 18.5, 18.9, 19.6 and 22.0 days when reared with the 1^st instar, 2^nd instar, initial 3^rd and old 3^rd fly larvae, respectively, and the corresponding immature survival rates were 16.40%, 46.88%, 54.09% and 26.29%, respectively. The development of D. longicaudata was greatly blocked at the 1^st instar and old 3^rd oriental fruit fly larvae. Body size of offspring wasps mainly depended on host’s ages, and the smallest wasps were observed from those 1^st instar oriental fruit fly larvae. However, a female-based sex ratio of offspring wasps was only exhibited in the initial 3^rd instar oriental fruit fly larvae, and the ratio of female offspring wasps was 64.11%.

Abstract:The optimal sterile concentration range of HMPA (hexamethyl phosphoryl triamide) against Bactrocera dorsalis was tested by feeding B. dorsalis adult with different concentration of HMPA dissolved in honey water. When normal female mates with the male treated with 0.05%HMPA, the hatching rate of their laying eggs was about 6%. The eggs don’t hatch when males were treated with the concentration of 0.09%. However, treatment of female adults with 0.07% HMPA resulted in a complete sterility. Based on the above results, the sterile effect of HMPA dissolved with 5% protein hydrolysate against B. dorsalis was studied by feeding B. dorsalis adult with concentration 0.02%, 0.04%,0.06%,0.08% and 0.10%. The results indicated that fecundity decreased with the concentration. The egg hatching rate was only 14% after feeding the males with 0.08% HMPA. But the egg hatching rate in the control group was up to 76%. The female adults were sterilized completely when the concentration was 0.08%.

Abstract:Cacopsylla citrisuga Yang & Li is a major pest of Citrus limon (L.) Burm.F. in Dehong lemon production region, Yunnan Province. The pest damages the young leaves and flushes of lemon trees. A recent study indicated that C. citrisuga was a carrier of Candidatus Liberibacter asiaticus, the bacterium associated with Asian type huanglongbing. This work was therefore initiated for study of the morphological characters, biology, population dynamics and economic damages of the psyllids in Dehong region. The study indicated that C. citrisuga preferred areas with an altitude between 1 000-1 500 meters. Its host plants were limited to the species of in genus Citrus as well as Poncirus trifoliate only. The pest caused more severe and a longer period of damage as altitude increased. The nymphs caused the most damage. The common symptoms of C. citrisuga infestation include distorted leaves. Population dynamics and occurrence of C. citrisuga are correlated with lemon flushing, altitude, and temperature.

Abstract:The inhibition effects and the disease-control efficiency of Allium tuberosum root extracts on Foc4(Fusarium oxysopoyum f.sp.cubence race 4), and root extracts effects on soil microbial ecology were studied by bacteriostatic experiment (mycelia growth rate method, spore germination method and pot trial). The results of bacteriostatic test indicated that A. tuberosum root extracts had significantly inhibition action on mycelia growth and conidium germination of Foc4, showing concentration effects on Foc4. In addition, the aberration of the morphology of mycelia and conidium of Foc4, which were impacted by A. tuberosum root extracts, was found by microscopic observation. The results of pot trial showed that the occurrence of banana fusarium wilt decreased significantly by A. tuberosum root extracts, and disease-control efficiency increased with the increase of root extracts concentration. When the root extracts concentration reached 160 mg·mL^-1, the disease-control efficiency was up to 53.66%. A. tuberosum root extracts could affect the numbers of Foc4 and the main microorgnanisms in the soil, which was presented as the numbers of Foc4, fungi and actinomyces in the soil were reduced, while the numbers of bacteria in the soil increased evidently. The Biolog analyses on the 40th planting days showed that A. tuberosum root extracts could noticeably increase carbon source utility by microbes and soil microbial community diversity, richness, uniformity, which manifested that A. tuberosum root extracts could adjust soil microbial community structure, enhance soil microbial ecological system stability and inhibition of disease, thus reducing the occurence of soil born disease.

Abstract:Morphological and molecular characterization of two putative Xiphinema americanum-group species (X. brevicollum and X. himalayense) from Yaccatree imported from Japan were described. ITS and D2D3 segments of two species were amplified, cloned, sequenced and analysed by using ME polygenetic tree. They were first intercepted by the General Administration of Quality Supervision Inspection and Quarantine of the People’s Republic of China. X. himalayense was reported in Japan and has no distribution record in China.

Abstract:The fusion expression strategy of human beta defensin 3(hβD-3) gene in Escherichia coli cells in the fusion expression by pET-32a(+) vector was studied. Fusion expression of F-βD-3 protein was performed in lysogenic bacterium BL21(DE3) by pET-32a(+) vector. The results showed that the production of F-βD-3 protein was 127.23 μg/mL. After nickel column chromatography and enterokinase cleavage, the fusion protein production finally obtained was 18.17 μg/mL. Recombinant hβD-3 protein products were identified to possess inhibitory activity by bacteriostatic test on E. coli K₁₂D₃₁.

Abstract:To explore the possibility of expressing the hybrid peptide CAD in the alfalfa, Medicago sativa, the cDNA sequence encoding CAD was obtained by recursive PCR(rPCR), and fused with elements such as Ω sequence, Kozak sequence and KDEL sequence to improve the expression level in the transgenic plants, and cloned into the plant transformation vector pCAMBIA1390R. This recombinant plasmid was transformed into Agrobacterium tumefacien LBA4404 and CAD gene was transformed into alfalfa by A. tumefacien-mediated method. The regenerated alfalfa plants were selected on medium 20 mg/L hygromycin. The results of PCR and Southern blotting showed that the CAD gene was successfully inserted into alfalfa genome. Proteins extracted from transgenic alfalfa showed a certain bacteriostatic activity to Salmonella and Staphylococcus aureus.

Abstract:The near isogenic lines of high and low lycoppene processing tomato were used as experiment materials; DNA was extracted from these plants to develop high and low lycoppene DNA gene pools, respectively. RAPD techniques were adopted to screen molecular marker linked to high lycoppene, and only primer S636 was found to be polymorphic between near isogenic lines of high and low lycoppene processing tomato from 260 RAPD random primers. It had been proved that a specific fragment of RAPD marker was linked to high lycoppene in the back cross parent and the single plants in the backcross population. The specific fragment was extracted from the agarose and ligated with pMD-18T vector, then transformed into DH-5α. The positive clone was sequenced to find a 827 bp fragment. This fragment could be used to develop SCAR marker for steady lycoppene.

Abstract:The floral development of the Theaceae species, Camellia semiserrata Chi. was observed under scanning electron microscope (SEM) for the first time up to date. Both perianth were initiated spirally and centripetally; in the species four to five carpel primordial arose from a ring meristem; in the flowering developmental process the carpels in C. semiserrata Chi. developed nearly into syncarpous gynoecium, and the carpels developed into connate stylus. The surface of stigma had a lot of papilla cells, and it was divided into 3-5 clefts. The stamen was divided into two wheels, and the internal stamen primordia all developed on a ring meristem; 15-21 stamen primordia in the inner whorl first arose simultaneously; the outer whorl of stamen primordia was divided into 3-5 layers; the same layer developed simultaneously on a ring meristem; different layers irregularly initiated; in the flowering development process the outer stamen primordia developed nearly into 1/3-1/2 of based connate stamens. The floral development of C. semiserrata Chi. is distinctly different from those of Adinandra latifolia(Theaceae) and Euryodendron excelsun(Ternstroemioideae, Theaceae). This study provides evidence from floral organogenesis about the further study on the phylogeny of Camellia and even of Theaceae.

Abstract:Floristic composition and species diversity of Baiwan Fengshui woods in the limestone region of Qingyuan City, Guangdong were studied by plot method. A total of 151 vascular plants were recorded in the 10 plots with an area of 400 m². They belong to 61 families and 119 genera. Among the 100 non-cosmopolitan genera, 79% are tropical distribution elements. They are dominated by pantropic elements. There were also higher percentage of old world tropic and tropic Asian elements. The results showed that there was the highest species richness in herbal layer, followed by shrub layer, and the lowest one in tree layer. There were the highest Simpson and Shannon indices in shrub layer, followed by herbal layer, and the lowest one in tree layer. Evenness was the highest in shrub layer, followed by tree layer, and the lowest one in herbal layer. Zenia insignis, Cinnamomum burmannii, Radermachera sinica, Adenanthera microsperma, Celtis sinensis, Chukrasia tabularis, Loropetalum chinense, Murraya paniculata, and Micromelum integerrimum were found to grow well in Fengshui woods. They can be used in rehabilitation of degraded limestone areas in Qingyuan City and even in other subtropical areas of Guangdong Province.

Abstract:This study deals with the changes of osmosis-regulating substances（proline, soluble sugar and soluble protein）of three common tree species in south China area, i.e., Evodia meliaefolia, Rhaphiolepis indica, and Zenia insignis, under drought stress by means of pot experiment, with an aim to prove the approach and mechanism of how the trial species adapt to the drought stress so as to provide evidence for species selection in south China limestone area. Major findings were as follows: in condition of drought stress, contents of proline and soluble sugar of all three species showed an increasing trend, which peaked under heavy drought stress. Among them, R. indica was the highest (proline 490.86 μg/g, soluble sugar 38.13 mg/g), while E. meliaefolia was the lowest (proline 278.33 μg/g, soluble sugar 27.74 mg/g), Z. insignis was moderate. The content of soluble protein approximated a parabola: first an increase, later a decrease. E. meliaefolia(5.97 mg/g) and Z. insignis(6.84 mg/g) peaked under light drought stress, while R. indica (7.44 mg/g) peaked in moderate drought stress. In condition of heavy drought stress, only R. indica’s content of soluble protein(53.64%) was higher than that of normal condition, which approximated a great osmotic adjustment ability. In conclusion, R. indica shows a great osmotic adjustment ability, which can well adapt to drought stress condition; Z. insignis takes the second place, and E. meliaefolia is the worst.

Abstract:Daily variations in photosynthesis and water-consumption in the seedlings were compared among three species by using Li-6400 photosynthetic system and by weighting potted seedlings. The experimental species included an endemic tree species (Zenia insignis) distributed in limestone area in south China and two other tree species (Evodia glabrifolia, Rhaphiolepis indica) commonly used for afforestation in limestone area. The results showed that R. indica and E. glabrifolia had similar or better ability to grow through photosynthesis and water conservation as compared with Z. insignis in vigorous growth periods. As for photosynthetic characteristics, the daily variation trend of net photosynthetic rate of seedlings from the three species was a “twin peaks” curve. R. indica had the highest net photosynthetic rate, reaching （11.709±0.392）μmol·m^-2·s^-1; E. glabrifolia took second place, and Z. insignis was at the lowest level. The parameter values on photoresponse curve indicated that R. indica had the most powerful ability to grow through photosynthesis, while Z. insignis and E. glabrifolia were similar to each other. As for the characteristic of water-consumption, the water-consumption in daytime and nighttime of Z. insignis was significantly higher than those of R. indica and E. glabrifolia. Water-consumption in daytime accounted for about 90% of the total consumption in the whole day. The water-consumption rate of the three species seedlings was comparatively low, between 2.91×10^-4 -4.28×10^-4 mol·m^-2·s^-1. Maximum water-consumption rate of E. glabrifolia was the lowest among them, while that of R. indica was slightly higher compared with Z. insignis, and the average ratio of the maximum water-consumption rate and the average water consumption rate was about 1.6.

Abstract:The purpose of this experiment was to explore the effects of sorbic acid(SA) on proliferation, IGF-1 secretion, GH/IGF system and glucose and lipid metabolism-related genes mRNA expression in C2C12 cells. C2C12 cells were exposed to different doses of SA(0, 0.1, 1.0, 10.0 and 100.0 μmol/L）to measure cell proliferation by MTT assay, IGF-1 secretion by RIA assay and transcript levels by qPCR assay. Results indicated that: 1) SA(10.0 μmol/L) significantly promoted(P<0.05) proliferation of cell cultured with 10% fetal bovine serum(1 and 2 d) or without serum(4 and 5 d). 2) IGF-1 secretion was significantly suppressed(P<0.05) by 0.1, 1.0 or 100.0 μmol/L SA in serum-free cultured C2C12 cells. 3) Expressions of IGF-1R, GHR and CPT1b mRNA were significantly up-regulated in serum-free cultured C2C12 cells treated with 1.0 μmol/L SA. The increasing trend of IGFBP3 (P=0.09), PDK4 (P=0.10) and PGC1 α(P=0.10) transcript levels were also observed in serum-free cultured C2C12 cells by 1.0 μmol/L SA treatment. This suggested that SA could increase proliferation and GH/IGF system and glucose and lipid metabolism-related genes mRNA expression but depress IGF-1 secretion in C2C12 cells.

Abstract:To investigate the relationship of anticancer effects, toxicity and dose, tumor syngeneic models were made from murine colorectal adenocarcinoma cell line CT26 derived from BALB/c mice. Mice were treated with various doses (10, 20, 30 and 50 mg/kg) of 5-fluorouracil (5-FU), and the indexes were analyzed, including the anti-tumorigenic effects, body mass and mortality of mice. The results showed that the efficacy and toxicity of 5-FU was closely related to the doses, and the proposal dose was 20-30 mg/kg which may be the optimal dose for drug research in tumor syngeneic model of mice.

Abstract:In order to investigate influence of DHA compound additives in diets on egg quality and production performance, 96 Hy-Line layers were divided into 4 groups at random, and each group consisted of 6 cages with 4 laying hens per cage. GroupⅠ was control group and fed with basal diet. Groups Ⅱ-Ⅳ were experimental groups and fed increasing levels of DHA compound additives at 2%,4% and 8% respectively. Results showed that average laying rate, average egg shape index and average feed/egg ratio of all groups were not significantly different(P>0.05); the average egg mass of groups Ⅱ-Ⅳ were found to be significantly higher than that of groupⅠ(P<0.01) in the whole trial period. Average haugh unit of group Ⅳ was also significantly higher than that of group Ⅰ in the first two weeks (P<0.01). In the third week, average haugh unit of group Ⅳ was significantly higher than that of the other three groups (P<0.05). In the last two weeks, average contents of DHA in groups Ⅱ-Ⅳ were significantly higher than those of group Ⅰ(P<0.01), whereas group Ⅳ was the highest. To sum up, the effects of DHA compound additive in diets can improve production performance and egg quality. According to the experiment results, the 8% DHA compound additive in diets is found to be the best approach.

Abstract:To understand the difference of the nucleoprotein (N) gene and glycoprotein (G) gene between the strain of pig origin and other rabies viruses. The N and G genes of rabies virus isolated from the brain of a rabid pig in Guangdong Province were amplified by RT-PCR, cloned, sequenced and compared with the genes of other rabies virus. The results showed that the sequence of nucleotides and amino acids of N genes of GD-SH01 shared 84.3%-98.0% and 92.5%-99.3% similarity，and G genes of GD-SH01 shared 80.4%-98.2% and 87.8%-99.6% similarity, respectively, compared to other viruses.

Abstract:Ninety Staphylococcus strains, including 19 Staphylococcus aureus and 71 coagulase-negative Staphylococci, were isolated from different swine lymph node samples collected from Guangdong Province in 2010. Antimicrobial susceptibility testing was performed with agar dilution method according to the recommendations of the Clinical and Laboratory Standards Institute (CLSI). PCR detection was used to screen 13 resistance genes among these strains. Statistical methods were used to analyze the differences in resistance and the distribution of resistance genes in S. aureus and coagulase-negative Staphylococci. The results showed that resistance rates of S. aureus and coagulase-negative Staphylococci to macrolides, lincosamides, tetracycline were very high(＞80%). There were no significant differences of antimicrobial resistance and the carriage rates of resistance genes between S. aureus and coagulase-negative Staphylococci, except that the coagulase-negative Staphylococci strains had a higher oxacillin resistance rate and higher limA and ermB gene carriage rates as compared with S. aureus（P＜0.05）.

Abstract:To study the pharmacokinetics and biavailibility of terbinafine hydrochloride capsules in beagle dogs, a single intravenous (i.v.) and oral(p.o.) administration of terbinafine at a dosage of 10 mg·kg^-1 was performed in eight healthy beagles according to a two-period crossover design. Plasma concentrations of terbinafine were determined by a reverse phase high performance liquid chromatographic method. The pharmacokinetic parameters were calculated by noncompartmental analysis with WinNonlin 5.2.1 software. After intravenous administration, the main pharmacokinetic parameters were as follows: AUC_0-∞ =(5.47±1.03) μg·mL^-1·h, V_ss =(2.55±0.89) L·kg^-1, CL=(1.88±0.33) L·h^-1·kg^-1, t_1/2 =(3.02±1.70) h; whereas after oral dosing, the main pharmacokinetic parameters were as follows: t_max =(1.09±0.37) h, C_max =（0.39±0.04）μg·mL^-1, AUC_0-∞ =(0.67±0.18) μg·mL^-1·h, V_d/F =(35.17±6.58) L·kg^-1,t_1/2 =(1.69±0.74) h. The absolute bioavailibilty(/F) of terbinafine hydrochloride capsules after oral administrtion was (12.54±3.43)%.Terbinafine was absorbed and eliminated rapidly in beagles and the absolute bioavailability was very low.

Abstract:To monitor the effectiveness of canine rabies vaccination, the indirect ELISA method had been developed to detect the neutralizing antibodies against rabies virus (RV) by using the main antigenic determinant of glycoprotein (RV G3) as coating antigen. The optimum test conditions were as follows: the optimal concentration of RV G3 coating the ELISA plate was 8 mg/L. The optimal concentration of serum samples and HRP-labeled goat anti-canine IgG was 1∶100 and 1∶3 000 respectively. RV G3 had no reaction with positive serum against canine distemper virus (CDV), canine adenovirus (CAV) or canine parvovirus (CPV) by the specificity test. The coefficients of variation of intro-batch and inter-batch duplicability test were fewer than 10%.The sensitivity was 1∶1 280.Compared with America SYNBIOTICS kit, the coincidence rate of indirect ELISA was 95.6%.Therefore, the indirect ELISA has good specificity, repeatability and sensitivity, which can be a good candidate for routine detection of neutralizing antibodies of canine rabies.

Abstract:An experiment was conducted to study the effect of different ways of micro-moist irrigation on growth and yield of maize under different buried depths, spacing and pressures of micro-moist irrigation. The results showed that the dry matter accumulation of micro-moist irrigation was different from that of drip irrigation; the stem diameter, height and yield increased with the water consumption; membrane drip irrigation had the highest yield which increased by 33.13%，34.48%，26.82% and 63.94%, compared with T1, T2, T3 and T4. Water use efficiency (WUE) of micro-moist irrigation was higher than that of drip irrigation, all treatments had no significant difference, WUE of T2 was the highest, and it had a significant difference with drip irrigation. Micro-moist irrigation was beneficial to the development of maize-seed and to the increase of grain mass. The effect of different micro-moist irrigation on the maize yield and WUE was significant under different buried depths, spacing and pressures. The pressure mainly influenced maize yield and WUE, and the spacing mainly influenced water consumption.

Abstract:The great temperature difference present between the front and the rear of current fresh-keeping transportation makes negative impact on the quality of fruit and vegetable. In order to resolve this problem, double temperature sensors were applied in temperature control system designed in this paper, which utilized one temperature sensor to measure the temperature of the rear of preservation chamber as the prior value in controlled, and utilized the other temperature sensor to measure the temperature of the front as the auxiliary value in control. The system proved a good feasibility, stability by experiments, which could not only control the temperature under the set value in the preservation chamber, but also keep the temperature over the temperature limit of fruits and vegetables. This result is valuable for the improvement of the technology of the fresh-keeping transportation.

Abstract:A new ternary copper(Ⅱ) complex: ［Cu(TBZ)(L-Ala)(H₂O)］ClO₄［TBZ=2-(4′-thiazolyl) benzimidazole, L-Ala =L-alaninate］, was synthesized and characterized by elemental analysis, molar conductivity, infrared absorption spectrum, and electronic absorption spectrum. The complex was assayed against gram-positive(Staphylococcus aureus, Bacillus subtilis) and gram-negative(Salmonella typhi, Escherichia coil) bacteria by double dilution method, and the interaction of the complex to DNA was investigated by electronic absorption spectrometry, fluorescence spectrometry, CD spectrometry, viscosity measurement and agarose gel electrophoresis. The results indicated that the complex had good antibacterial activity, which could be bound to CT-DNA (calf thymus DNA) by intercalative mode, cleave pBR322 DNA in the presence of vitamin C in the involvement of the hydroxyl radical, and possibly singlet oxygen.

Abstract:An experimental study was conducted to explore the effect of supercritical extraction conditions on the extraction rate of deer oil. Deer venison was used as raw material in this study. The extraction pressure, extraction temperature, and extraction time on the yields were studied by single factor experiments and orthogonal tests. The results showed that the highest extraction rate could be reached at an extraction temperature of 45 ℃, under an extraction pressure of 40 MPa, and the extraction time was 150 min. After the measuration of physical and chemical property indicators of the deer oil and the analysis of the composition of the fatty acid, it was found that the deer oil acquired through supercritical extraction had a good color and low acid value and contained a variety of fatty acids, of which the total unsaturated acids reached 41.94%.

Abstract:To screen the germplasm with heat tolerance in rice, twenty eight varieties (lines) from 11 countries were selected for evaluation of heat tolerance in this study. The tested rice varieties (lines) at flowering stage were subjected to high temperature treatment in phytotron with temperatures equal to or higher than 35.0 ℃ for 10 hours, and the daily average temperature had been 33.5 ℃ for 7 days. The results indicated that the heat tolerance of the tested varieties (lines) varied considerably. The percentage of spikelet fertility of the tested varieties ranged from 0.6% to 58.7％ and their heat tolerance indexes ranged from 0.01 to 0.85 after high temperature treatment. The indica varieties, “Ganxiangnuo” and “N22” were the most heat-tolerant varieties which had the heat tolerance indexes of 0.84 and 0.85, respectively. Twenty-four SSR and InDel markers that could specifically differentiate indica from japonica were used to analyze the differentiation of the tested materials. The degree of indica-type of the tested materials calculated by the ratio of the number of markers that could generate indica-type band pattern to the total number of markers used was positively correlated with their heat tolerance index (P<0.05), suggesting that indica subspecies was more heat-tolerant than japonica subspecies and there would be more chances to identify heat-tolerant rice germplasm when screening is conducted in indica rice.

Abstract:The genus of Chaeopsestis Le Cerf, 1941 and the species of C. ludovicae Le Cerf, 1941 from China were reported for the first time. Images of adult and male genitalia were illustrated. The specimens are deposited in Department of Entomology, College of Natural Resources and Environment, South China Agriculture University.

Abstract:Three species of invasive plants are reported as new records to Hainan Province, China, i. e. Macroptilum atropurpureum (DC.)Urban, M. lathroides (L.) Urban, and Desmodium tortuosum (Sw.) DC. Voucher specimens are deposited in the Herbarium of Forestry College, South China Agricultural University(CANT).