SHI Ruyi , ZHANG Xiujuan , BAI Yinshan , WEI Hengxi , LI Li , ZHANG Shouquan
2014, 35(2):1-5. DOI: 10.7671/j.issn.1001-411X.2014.02.001
Abstract:【Objective】The aim of this research was to optimize the cryopreserving conditions of porcine spermatogonial stem cells (PSSCs) and observe the changes of ultrastructure in PSSCs.【Method】The testicular cells of Landrace piglets aged from 1 day to 5 days were isolated by two-step enzymatic digestion method. The percoll discontinuous density gradients method was used to purify PSSCs. The transmission electron microscope (TEM) was used to observe the ultrastructure of PSSCs during different culturing periods in vitro. The effects of glycerine and dimethyl sulfoxide (DMSO) on PSSCs cryopreserving were investigated.【Result and conclusion】The results showed that the cell membrane of undifferentiated PSSCs was integrated and smoothed without pseudopodia, the cytoplasm was homogeneous and nucleus was clear. The pseudopodia were formed in differentiated PSSCs and the number of the mitochondria greatly increased. The appropriate cryopreserving medium for PSSCs is V(DMSO)∶V(DMEM/F12)∶V(FBS)∶V(100×Penicillin/Streptomycin solution) =10∶69∶20∶1.
JIANG Zhuying , LI Lili , ZHANG Bin , TANG Lihua , LIU Zhiqiang , DUAN Jielin , YIN Yulong , LI Tiejun
2014, 35(2):6-12. DOI: 10.7671/j.issn.1001-411X.2014.02.002
Abstract:【Objective】This experiment was conducted to study the effects of adding bamboo-carbon and bamboo vinegar to the diets contaminated by deoxynivalenol (DON) on serum free amino acids content of weaned piglets.【Method 】A total of 28 crossbred (Duroc×Landrace×Large white) weaned piglets with an average body mass of (12.10±1.12) kg were randomly assigned to 4 groups, with 7 replicates per group and 1 piglet in each replicate. The 4 groups were as follows: control group (a basal diet), DON group (the basal diet+DON), bamboo-carbon group (the basal diet+DON+2% bamboo-carbon), bamboo vinegar group (the basal diet+DON+1% bamboo vinegar). The experimental period lasted for 37 d. 【Result and conclusion】 1)On the 15th day of the experiment, there was no significant difference between the experiment group and control group (P>0.05), besides 3MHis in DON group and bamboo-carbon group significantly decreased by 39.06%,40.62%(P<0.05). 2)On the 30th day of the experiment, compared with the control group, Ile, Val, Cys, Hyp, 3MHis in DON group were significantly decreased by 26.60%, 25.96%, 73.38%, 44.61%, 26.09%(P<0.05)as compared to the control group, Cys, 3MHis in bamboo-carbon increased by 173.17%, 8.82%(P>0.05), respectively; Hyp, 3MHis Cys in bamboo vinegar group increased by 55.96%(P<0.05), 26.47%(P<0.05), 170.73%(P>0.05), respectively. 3)On the 37th day of the experiment, compared with the control group, Hyp in DON group significantly increased(P<0.05); His, 1MHis in bamboo-carbon group significantly decreased(P<0.05); His, Orn, 1MHis in bamboo vinegar group significantly decreased(P<0.05); Hyp, Gln in bamboo vinegar group significantly increased(P<0.05). Dietary supplementation with 2% bamboo-carbon or 1%bamboo vinegar could alleviate a part of serum free amino acids content, with a decrease or a significant decrease by DON challenge in weaned piglets. The weaned piglets have certain tolerant ability to DON with the time, dietary supplementation with bamboo-carbon and bamboo vinegar can give appropriate adjustment.
SHI Junsong , TIAN Cunfeng , XU Jiguo , ZHANG Xiujuan , LI Li , ZHANG Shouquan
2014, 35(2):13-17. DOI: 10.7671/j.issn.1001-411X.2014.02.003
Abstract:【Objective】To analyze the phylogenetic relationship of South China tiger, Panthera tigris amoyensis , Siberian tiger, P.tigris altaica and Bengal tiger, P.tigris tigris . 【Method】The fragment sequences of three mitochondrial genes were achieved by sequencing the PCR clones. These included CO Ⅰ 750 bp, CO Ⅲ 737 bp and ND4561 bp based on the three gene sequences analyses with the DNAStar software and the DNAssit software. Their base substitutions, Kimura 2-parameter distances and the percentage differences among three tiger subspecies were obtained. At the same time, the three genes sequences had fewer mutation sites. In addition, the Kimura 2-parameter distances of tiger subspecies was studied, selecting the mtDNAs of cat, Felis catus, leopard, Acinonyx jubatus and clouded leopard, Neofelis nebulosa as outgroup. The Mega 4.0 was used to reconstruct the evolutionary relationship of South China tiger, Siberian tiger, Bengal tiger with a neighbor-joining method. 【Result and conclusion】 The results of the Kimura 2-parameter distances of tiger subspecies indicates that Siberian tiger is closer to Bengal tiger than to South China tiger. The results of the CO Ⅰ, CO Ⅲ and ND4 genes show that Siberian tigers and Bengal tigers evolve from South China tigers.
SHU Jianhua , GUO Hongyan , WANG Liqi , GAO Yan , ZENG Zhenling
2014, 35(2):18-23. DOI: 10.7671/j.issn.1001-411X.2014.02.004
Abstract:【Objective】To establish a high sensitivity enzyme-linked immunosorbent assay for the detection of furaltadone.【Method】Furaltadone metabolite (3-amino-5-morpholinomethyl-2-oxazolidinone, AMOZ) was coupled to carrier proteins (bovine serum albumin or ovalbumin) and reduced with NaBH4 for the production of immunogen AMOZ-BSA and coating antigen AMOZ-OVA through glutaraldehyde method. With 4-carboxybenzaldehyde for derivatization reagent, AMOZ was made into CPAMOZ, which was successfully conjugated to carrier proteins according to the active ester method to form CPAMOZ-BSA, CPAMOZ-OVA. UV scanning showed that antigens were successfully linked to carrier proteins. After immunizing animal (Balb/c mice), polyclonal antibody against NPAMOZ was produced.【Result and conclusion】 Antibody was diluted at 1∶32×104 and the IC50 value was 4.53 ng·mL-1. The cross-reactivity (CR) of the antibody with CPAMOZ, AMOZ and furaltadone was 78.6%, 1.5% and 4.6% respectively. There is almost no CR with other three nitrofurans and their metabolites, which indicates a high selectivity of the antibody.
LI Bo , ZHAO Jixiang , CHEN Zhangliu , LU Wenhua , HAN Keke , XU Wei , CHEN Hong
2014, 35(2):24-27. DOI: 10.7671/j.issn.1001-411X.2014.02.005
Abstract:【Objective】A study on the diminazene-protein binding rate in swine was carried out in this experiment for further clinical application efficiency and safety.【Method】The plasma samples were pretreated to separate free drug by ultrafiltration. A high performance liquid chromatographic-UV (HPLC-UV) method was used to measure the amount of free diminazene in plasma. Then the drug-protein binding rate was accounted. Six swine were administrated intramuscularly at a dosages of 7 and 14 mg/kg. The plasma samples were collected and the protein binding rates in vivo were determination at 3, 5, 15, 60 min.【Result and conclusion】Diminazene-protein binding rate tested in vitro was within the range of 74.68%-95.90%. Although the drug-protein binding rate in vivo was within the range of 84.11%-89.69%(7 mg/kg, i.m.), and 82.96%-88.65%(14 mg/kg, i.m.) after administration, and saturation phenomenon emerged as dosage increased. These factors should be considered in clinical regimens designing.
YU Han , WU Chunsheng , WANG Zhenmin , CHEN Xifeng , GU Yan
2014, 35(2):28-34. DOI: 10.7671/j.issn.1001-411X.2014.02.006
Abstract:【Objective】Effects of soybean continuous cropping on soil microorganisms and physicochemical properties were studied.【Method】With the rhisphere soils of cropping and rotation soybean as test materials, the following indexes including soil microorganisms, bacterial physiological groups, physicochemical properties were investigated during the different growth stages of soybean.【Result and conclusion】The results showed that there were significant influences of continuous and alternate cropping on soil microorganisms. The number of bacteria, actinomycetes decreased, especially the reduction of ammonifying bacteria, nitrifying bacteria, aerobic nitrogen-fixing bacteria, cellulose-decomposing bacteria, but fungi amount increased. The content order of soil available nitrogen and potassium was rotation cropping>alternate cropping>1-year continuous cropping>2-year continuous cropping >3-year continuous cropping. The content order of soil available phosphorus was rotation cropping>alternate cropping>1-year continuous cropping>3-year continuous cropping >2-year continuous cropping. The mean mass diameter and geometric mean diameter of rotation soil were significantly higher than alternate and continuous cropping.
WANG Lijing , JIANG Dingxin , CHEN Zepeng , WEI Jianyu , WAN Shuqing , MA Zhuwen
2014, 35(2):35-39. DOI: 10.7671/j.issn.1001-411X.2014.02.007
Abstract:【Objective】To improve the quality of the flue cured tobacco leaf by combined application of chelated fertilizer. 【Method】Effects of combined application of chelated fertilizer on the contents of total sugar, reducing sugar, total nitrogen, nicotine and potassium of flue-cured tobacco leaf were studied using the pot and field experiments. Effects of chelated fertilizer of Kangkai Ⅱ, Funuogen, Funuo potassium or Funuo phosphorus on the quality of tobacco leaf were analysed. 【Result and conclusion】The results showed that in plot experiments, application of Kangkai Ⅱ and Funuogen at the stage of seedling, Funuo potassium or Funuo phosphorus at the stage of growing reduced the contents of tobacco leaf nicotine by 42.86% and 26.02% and increased the potassium contents by 41.24% and 3.44%. In field experiments, The combined application of Kangkai Ⅱ, Funuogen, Funuo potassium or Funuo phosphorus reduced the contents of tobacco leaf nicotine by 56.00% and 93.28%, increasing the potassium contents by 23.11% and 29.25%,respectively.
GAO Yali , LIU Jinshan , GUAN Lilan , ZHANG Xinming
2014, 35(2):40-46. DOI: 10.7671/j.issn.1001-411X.2014.02.008
Abstract:【Objective】The method of available phosphorus characterized by phosphorus extracted in KCl dilute-salt solution was studied. 【Method】 By using standard solution and soil samples from different land use patterns, influences of different conditions during extracting and color-developing stages on the precision and accurancy of dilute-salt solution-malachite green method were studied. 【Result and conclusion】 The optimum conditions for extraction were as follows:1 g soil∶20 mL water for soil-water ratio, 0.5 h for oscillation, and 0.02 mol·L-1 for concertration of KCl extracting solution; The optimum final concentrations of reagents for the color-developing system were as follows: H2SO4 0.48 mol·L-1,(NH4) 6Mo7O24·4H2O 0.024 mol·L-1, malachite green 0.06 g·L-1. The association complex had a maximum absorption wavelength at 620 nm, and Beer’s law was obeyed in the P range of 0~0.2 mg·L-1 with the linear regression equation being A =3.894 3C+0.001(R2 =0.999**)and P detection limit being 0.002 mg·L-1, and the method had high accurancy and precision as well. Thus the method is appropriate to determine phosphorus amount in KCl extracting solution.
CHEN Xiu , RAO Xueqin , RUAN Xiaolei , LI Huaping
2014, 35(2):47-52. DOI: 10.7671/j.issn.1001-411X.2014.02.009
Abstract:【Objective】To provide antibody of Banana streak virus Guangdong isolate (BSV-GD) and to provide references for the function research of proteins encoded by BSV ORF3 further. 【Method】The MP functional gene sequence was obtained through bioinformatics analysis. The gene was cloned and inserted into the plasmid pET-28b(+) to construct the prokaryotic expression recombinant plasmid. The recombinant vector was induced by IPTG to express the fusion protein 6His·MP. Soluble analysis of the fusion protein was carried out by ultrasonic lysis method. The highly purified protein was obtained by His-tag purification kit. The special polyclonal antibody was generated by immunizing healthy rabbit using the purified protein as antigen. The specificity of antiserum was detected by Western-blot. The antibody titer was determined by indirect enzyme immunoassay. 【Result and conclusion】The analysis showed that the MP functional gene was 61-311 aa of the ORF3 sequence and the nucleotide sequence length was 753 bp. The prokaryotic expression recombinant plasmid pET28b-MP was constructed, and the expressed fusion protein 6His·MP was about 30 800 in size. Soluble analysis of the fusion protein indicated that it was an inclusion body. The highly purified target protein was obtained. The special polyclonal antiserum was generated according to the purified protein. The assay suggested that the antiserum had very strong specificity, and the antibody titer was higher than 1∶204 800.
SUN Jie , WANG Wan , ZHOU Ling , RUAN Xiaolei , RAO Xueqin , LI Huaping
2014, 35(2):53-56. DOI: 10.7671/j.issn.1001-411X.2014.02.010
Abstract:【Objective】To develop a real-time quantitative PCR method with TaqMan probes to quantify Cucumber mosaic virus(CMV) in banana.【Method】The primers and probes were designed based on the conserved coat protein(CP) sequences of CMV and were applied to real-time PCR assays.The reaction system was optimized,and its sensitivity, specificity and repeatability were evaluated.【Result and conclusion】The detection sensitivity of the real-time PCR assay was 4.2×102 μL-1, which was 100 times more sensitive than PCR. The specifity of the assay was analyzed with Banana bunchy top virus(BBTV) and Banana streak virus(BSV), and no cross reaction were observed. The assay also had good repetitions. The real-time PCR assay was evaluated with field samples. 5 of the 14 tissue samples collected from field suspected CMV infected bananas were positive, which further confirmed that the real-time PCR method should be suitable for detection and quantitation of CMV in banana.
REN Yanling , LIU Bin , CHEN Yanlu , LI Yongjiang
2014, 35(2):57-60. DOI: 10.7671/j.issn.1001-411X.2014.02.011
Abstract:【Objective】To identify the Orbilia orientalis collected from dead branches. 【Method】Its anamorph was isolated from the ascospores of Orbilia orientalis. 【Result and conclusion】The anamorph was identified as Drechslerella cf. brochopaga.The connection between anamorph Orbilia orientalis and teleomorph Drechslerella cf. brochopaga was established.
LIANG Shejian , LIANG Jintang , JIANG Ningxiong , LIU Peiwei , WU Hong
2014, 35(2):61-65. DOI: 10.7671/j.issn.1001-411X.2014.02.012
Abstract:【Objective】 The study of secretory cavity development and essential oil in the fruit of Citrus reticulata may provide scientific basis of morphology and phytochemistry for timely harvest of Chenpi. 【Method】The developmental processes of fruit and secretory cavity were studied by paraffin section and semi-thin section method. The change of essential oil during fruit development was quantitatively analyzed. 【Result and conclusion】 From May to January, the fruit of C. reticulata developed from hard and dark green to soft and golden yellow or orange. In the epicarp, the secretory cavities were distributed in a ring with different depths. The developmental processes of secretory cavity were divided into four stages. The secretory cavity was shown to be composed of two areas: a cap area and a circular area. In the center of the latter, one big cavity was surrounded by 2-5 layers of epithelial cells. Essential oil secreting from the epithelial cells was stored in this cavity. With the fruit development, the accumulation of essential oil followed a “S” trend. The ratio of essential oil displayed a gentle rise during the period from May to August, a rapid growth from September to November, reaching a peak in the middle of November, and a slight decline in early December. It is suggested that the medically effective fraction of Chenpi is the epicarp. The best time for harvest should be from late Novermber to early December. In this period, the fruit color is light yellow or golden yellow. The average diameter of secretory cavities reaches the maximum (200 μm), and the ratio of essential oils is also the highest (8.2%-8.5%).
PENG Guixiang , LU Qiuyan , KONG Huiqing , LI Xiong , WAN Tao , LU Yusheng , TAN Zhiyuan
2014, 35(2):66-72. DOI: 10.7671/j.issn.1001-411X.2014.02.013
Abstract:【Objective】 This study aimed at isolating and screening of efficiently biological flocculation strains from the paddy field of the planted wild rice.【Method】A high bioflocculant strain YH39 was obtained from the screening of kaolin suspension flocculation effects of 135 strains isolated from Oryza officinalis on VM medium.【Result and conclusion】16S rDNA sequences analysis showed that the strain YH39 was closely related to the known species Burkholderia cepacia with 98.6% similarity. The best carbon and nitrogen sources for producing flocculation were glucose and potassium nitrate, respectively. The fermentation kinetics analysis showed that strain YH39 reached the highest values of biomass concentration and flocculating activity in 16 h. Flocculant composition analysis indicated that the bioflocculant components were polysaccharide with good heat resistance, which was heated at 60 ℃ for 30 min without losing flocculating activity. The application of bioflocculation of three kinds of dyeing wastewater show that the fermentation liquid has good flocculating effects on dyeing wastewater with effective decolorization and reduces CODCr in dyeing wastewater.
ZHANG Lu , JING Xiaoli , SU Zhiyao , DU Weijing
2014, 35(2):73-77. DOI: 10.7671/j.issn.1001-411X.2014.02.014
Abstract:【Objective】The objective was to determine the altitudinal pattern of Rhododendron plant distribution and the potential of using indigenous Rhododendron plant resource in landscape greening.【Method】 The continuous transect sampling method was employed and a total area of 15 600 m2 was surveyed. A horizontal transect (10 m×120 m) was placed at an 100 m altitudinal interval from 700 m to 1 900 m a. s. l., representing the altitudinal range of Rhododendron in Nanling mountains of north Guangdong. The contiguous grid quadrat sampling method was used for plant census in each transect, which consisted of 12 quadrats (10 m×10 m). Correlation analysis and two-way indicator species analysis (TWINSPAN) were used to analyze the altitudinal patterns of Rhododendron species.【Result and conclusion】 Seven Rhododendron species were found in the 15 600 m2 plot, all of which were perennial evergreen woody shrubs or small trees. These Rhododendron species were divided into three categories by TWINSPAN. The first category was R. championae, R. simiarum and R. chunii; the second was R. cavaleriei, R. kwangtungense and R. bachii, and the third was R. fortunei. These TWINSPAN results indicated that environmental factors influenced the distribution and ecological characteristics of Rhododendron species. The most widely distributed species in an altitudinal spectrum is R. cavaleriei, followed by R.bachii and R. championae. The altitudinal patterns of these native Rhododendron species reflect their bioclimatic adaptation and phenology, which have potentials for use in landscape greening.
CHEN Yujun , LIAO Baowen , LI Mei , DENG Zhihong , WEI Junfa , GUAN Wei
2014, 35(2):78-85. DOI: 10.7671/j.issn.1001-411X.2014.02.015
Abstract:【Objective】Growth adaptability of different mangrove species to the environmental influence on the high-salinity beach (higher than 30‰) was determined in order to provide reference and basis for mangrove afforestation on difficult sites. 【Method】Growth difference of mangrove species was analyzed through field planting experiments on high-salinity beaches in relation to different beach elevations, soil types and planting measures. 【Result and conclusion】 Beach elevation had significant effects on the growth and survival rate of mangrove species Rhizophora stylosa, Kandelia obovata, Laguncularia racemosa and Sonneratia apetala on high-salinity beaches. The growth and survival rate reached maximum at mean sea level beach elevation generally,while they showed a declining trend with the beach elevation above or below mean sea level. At the sandy beach with elevation at mean sea level, L. racemosa and S. apetala had a survival rate of over 80%, and the growth amount of L. racemosa was much larger than R. stylosa, K. obovata and S. apetala. At the muddy beach with elevation at 0.2 m above mean sea level, the survival rate of both R. stylosa and K. obovata was over 70% while the survival rate of both L. racemosa and S. apetala was lower than 20%. As for the growth amount, L. racemosa was close to S. apetala, a little larger than R. stylosa and K. obovata. Marine algae affected the growth of mangrove plants in winter and spring. Saplings could suffer heavily especially in the windward area, among which the survival rate of R. stylosa and L. racemosa in the windward area was only 40% of that in the leeward side. At sandy site the growth and survival rate of R. stylosa saplings originated from hypocotyl was much higher compared with those originated from seedlings, but at muddy sites, growth difference of R. stylosa saplings with different origins was relatively small. As for planting measure, planting depth of hypocotyl within a certain range did not influence the growth and survival of mangrove saplings.
SUN Jin , WANG Xiaojing , GAO Zhenzhong , LIN Yan , PENG Yougui , XU Zhengchun
2014, 35(2):86-89. DOI: 10.7671/j.issn.1001-411X.2014.02.016
Abstract:【Objective】 The study of wood anatomical features of Sonneratia apetala growing in Leizhou could provide a basis of identification. Analyzing its fiber morphology could evaluate whether it would be suitable for paper-making. 【Method】Wood anatomical features of Sonneratia apetala were observed by means of optical microscope and scanning electron microscope. 【Result and conclusion】The results indicated that S. apetala had diffuse porous wood with few solitary and multiple pores. The perforation was simple. The pitting between vessels was alternate and vestured. The pitting between vessel and ray showed round and few reniforms. The fiber was libriform fiber. The ray tissue was homogeneous homocellular uniseriate ray with rich crystals. The longitudinal parenchyma was scattered. The quantitative characteristics of fiber indicate that S. apetala is suitable for paper-making, but it cannot be used alone for the making of high tear-resistance paper due to its short fibers.
WANG Lu , DUAN Ranran , ZHAO Pan , ZHOU Xiaohua
2014, 35(2):90-95. DOI: 10.7671/j.issn.1001-411X.2014.02.017
Abstract:【Objective】In order to design and find specific recognition and cleavage agent of DNA, two copper(Ⅱ) complexes,[Cu(AMB)2Cl]Cl·4H2O(complex 1)and[Cu(AMB)(phen)Cl]Cl·2H2O(complex 2)(AMB = 2-aminomethylbenzimidazole, phen=1,10-phenanthroline), were synthesized. 【Method】The two complexes were characterized by elemental analysis, IR, UV and molar conductivity. The complex 1 and complex 2 were assayed against gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and gram-negative bacteria(Escherichia coil and Salmonella typhi) by a double dilution method, and the interaction with ct-DNA was investigated by electronic absorption spectroscopy, fluorescence spectroscopy, viscosity measurements and agarose gel electrophoresis.【Result and conclusion】The complex 2 could interact with DNA by non-intercalation, while complex 1 by non-intercalation, and they cleave pBR322 DNA assisted by vitamin C involving the hydroxyl radical oxidation mechanism. The order of combination ability with ct-DNA and cleaving pBR322 DNA of the two complexes is complex 2>complex 1, which is in accordance with the bacteriostatic activity.
ZANG Ying , LUO Xiwen , ZHANG Guozhong , ZENG Shan , WANG Pei , ZHANG Minghua , CHEN Xiongfei
2014, 35(2):96-100. DOI: 10.7671/j.issn.1001-411X.2014.02.018
Abstract:【Objective】To study the effects in the precision rice hill-drop drilled field on methane emission.【Method】 Seven treatments were designed, including three precision rice hill-drop drilling types: the first precision rice hill-drop drilling with deep fertilization(30 cm), the second with the alternate line(35 cm+15 cm) and the third with fixed line(25 cm),using mechanical transplanting, manual seedling broadcasting and manual transplanting as the control groups. Methane samples emitted from paddy field in 2010 and 2011 were collected using a closed-chamber technique. 【Result and conclusion】 The results showed that CH4 flux was reduced significantly in paddy fields treated by the precision rice hill-drop drilling compared with the non-precision rice field control within three weeks on the rice early tillering stage (reduced by 54.55%, P=0.011). Their flux order of CH4 emission were as follows, the first precision rice hill-drop drilling with deep fertilization type[(1.48±0.44) mg·m-2·h-1]< the second with the alternate line[(1.65±0.30) mg·m-2·h-1]< the third with fixed line[(2.57±0.56) mg·m-2·h-1]< manual seedling broadcasting[(2.89±0.61) mg·m-2·h-1]< mechanical transplanting[(3.77±0.64) mg·m-2·h-1]< manual transplanting[(4.76±0.90) mg·m-2·h-1]< manual broadcasting[(5.33±0.84) mg·m-2·h-1 ].The present study results showed that the precision rice hill-drop drilling technology can reduce the emission of CH4 in the pre-tillering paddy field effectively.
CAO Liying , YAO Yuxia , YU Helong , MA Li , CHEN Guifen
2014, 35(2):101-104. DOI: 10.7671/j.issn.1001-411X.2014.02.019
Abstract:【Objective】 To construct a fuzzy ontology of maize disease knowledge representation and reasoning model.【Method】Using data from sources to construct fuzzy ontologies,which were combined with fuzzy theory to construct the disease diagnosis model by ontology technology. 【Result and conclusion】The theory of fuzzy was introduced into the description of ontology form and the shortcoming of an ontology representing the narrow defined knowledge only was overcame by this model. Combining the formal definition of ontology with the indeterminacy presentation advantage of the theory of fuzzy, the robustness of maize expert system for diagnosis and treatment of diseases was increased. Meanwhile,this model improved the accuracy of diagnosis and treatment of maize diseases and presented a new knowledge representation reference for other agricultural production expert systems.
ZHANG Zhengping , CHENG Ziyong , SHEN Guoyun , ZHANG Rui
2014, 35(2):105-109. DOI: 10.7671/j.issn.1001-411X.2014.02.020
Abstract:【Objective】 To construct an optimal water resources allocation model.【Method】This study chose the water resources of the Yellow River for Gansu Province as the research object. The optimal technique of water resources management decision support system was used to construct the model of optimal allocation of water resources through years of water supply and demand balance analysis.【Result and conclusion】Model calculation results showed that in the 2020 and forward 2030 time period, water resources allocation would be 5.275 billion cubic meter and 6.452 billion cubic meter respectively, including a reduction in agricultural water for 280 million cubic meter and 70 million cubic meter compared to their last decade’s respectively, and other industry water consumption would increase to some extent. This scheme can meet region’s socioeconomic and ecoenvironmental development demands for water resources according to the prediction.
CHEN Lanying , LI Yunxiang , QIAN Yifan , QUAN Qiumei
2014, 35(2):110-114. DOI: 10.7671/j.issn.1001-411X.2014.02.021
Abstract:【Objective】To explore the inter and intraspecifically physiological and biochemical characteristics of three barrenwort species and to provide theoretical basis of standardized cultivation with high yield.【Method】 The morphology, the relative content of chlorophyll(SPAD value) and stomatal conductance of three transplanted barrenwort species were investigated. Non-parametric test and one-way ANOVA were used to analyze the data. 【Result and conclusion】 The order of plant height with young leaves from high to low was Epimedium wushanese Ying, Epimedium pubescen Maxim, Epimedium acuminatum Franch. And there were significant differences between E. acuminatum and the other two plants. The order of plant height with old leaves from high to low was E. wushanese, E. acuminatum, E. pubescen. And there were significant differences between E. pubescen and the other two plants. Significant differences of leaf morphology of plants with old leaves were observed among three species. But no significant differences was observed among plants with young leaves of three species. For three species, the chlorophyll contents of all plants with old leaves were higher than those of plants with young leaves. Significant differences of stomatal conductance were observed among three intraspecifically species with young leaves were slightly higher than those of old leaves. The different barrenwort species had different degress of adaptability to the growth environment.
2014, 35(2):115-116. DOI: 10.7671/j.issn.1001-411X.2014.02.022
Abstract:【Objective】To study the nature of flora in South jiangxi. 【Method】Plant specimens were collected and identified. 【Result and conclusion】The genus Blainvillea Cass. (Asteraceae) and the species Blainvillea acmella (Linn.) Phillipson were reported as new records in Jiangxi Province, China. Voucher specimens are deposited in Dendrological Herbarium of the Forestry College, South China Agricultural University (CANT).
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