• Volume 35,Issue 5,2014 Table of Contents
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    • >Animal science
    • Isolation and identification of chicken adipose-derived mesenchymal stem cells

      2014, 35(5):1-7. DOI: 10.7671/j.issn.1001-411X.2014.05.001

      Abstract (1781) HTML (0) PDF 1.01 M (2705) Comment (0) Favorites

      Abstract:【Objective】 A method for isolation and identification of chicken adipose-derived mesenchymal stem cells(AMSCs) was established.【Method】 AMSCs from Tianlu yellow chickens were obtained by type I collagenase digestion. CCK-8 was used to detect cell activities. RT-PCR was used to examine their specific marker. Whereas their adipogenic and osteogenic differentiations were chemically induced.【Result and conclusion】The primary cultured and subcultured cells showed fibroblast-like morphology, and primary AMSCs were subcultured to passage 10 without any change in activities. The growth curves were typically sigmoidal. RT-PCR assays showed that the specific markers of AMSCs, CD29, CD44 and CD71, were positive, but CD34 and CD45 characterized by hematopoietic stem cells were negative. In addition, AMSCs can successfully differentiated into osteoblasts and adipocytes in different media. Lipid droplets formation was recorded during adipogenic induction, with the cells being positive for oil red O staining, and mRNA expression of peroxisome proliferator-activated receptor-γ(PPARγ) and fatty acid(FAS)was increased. During osteogenic induction, alizarin red staining showed that the calcium nodus was positive, and there was significant difference in alkaline phosphatase (ALP) activities between the test and control group (P<0.05), mRNA expression of ALP and bone morphogenetic protein-2 (BMP2) also increased. This research suggests that the mesenchymal stem cells isolated from chicken adipose tissues are multi potential and may provide the possibility for future clinical application.

    • Effects of roxarsone expo to carbon-source metabolic capacity and functional diversity of soil microbial communities

      2014, 35(5):8-13. DOI: 10.7671/j.issn.1001-411X.2014.05.002

      Abstract (1712) HTML (0) PDF 463.62 K (2169) Comment (0) Favorites

      Abstract:【Objective】 To evaluate the effects of roxarsone residues on the microbiological characteristics of soil, the carbon-source metabolic functions of the soil microbial communities exposed to roxarsone and studied by Biolog technology.【Method】 Fresh soil with different concentrations of roxarsone solution mix were collected so that drugs were (as As): group 15 mg·kg-1, group 75 mg·kg-1, group 150 mg·kg-1, cultivated at room temperature (20-25 ℃). Soil samples were taken after 1,2,3,5,8 weeks of treatment for analysis using Biolog method.【Result and conclusion】 The results showed that the carbon-source metabolic functions of the soil microbial communities of groups treated with roxarsone were significantly distinguished from that of CK, indicating a dose-dependent relationship. The carbon-source metabolic functional diversity of soil microbial communities in the lower roxarsone groups (w=15 mg·kg-1) were not significantly different from the control group during the 1st week. However, the soil microbial community functional diversity in the four groups (control, low, medium and high group) was significantly different in the 2nd, 3rd, 5th and 8th week. The difference between control and treatment groups appeared to be much more severe with the increase of roxarsone concentration. It is concluded that the carbon-source metabolic capacity and functional diversity of soil microorganisms in roxarsone treatment groups change with the increase of roxarsone concentration. In the 2nd week and 3rd week, the functional diversity of the soil microbial community of the three roxarsone groups decrease more rapidly compared with that in the 5th week and 8th week, which might be caused by the chemical conversion and degradation of roxarsone in soil.

    • An optimization study on determination of fluoroquinolones residues in chicken muscle by high performance liquid chromatography

      2014, 35(5):14-18. DOI: 10.7671/j.issn.1001-411X.2014.05.003

      Abstract (1483) HTML (0) PDF 364.40 K (2326) Comment (0) Favorites

      Abstract:【Objective】A method for rapid determination of five fluoroquinolones (norfloxacin,ciprofloxacin,danofloxacin,enrofloxacin,sarafloxacin) residues in chicken muscle using high performance liquid chromatography (HPLC) was established.【Method】The extracting solution of the chicken samples included a mixture of acetonitrile and trichloroacetic acid(the mass fraction of 2%). HPLC mobile phase consisted of acetonitrile and phosphate buffer (0.02 mol·L-1) with triethy lamine. Fluorometric excitation wavelength was 280 nm, and emission wavelength was 450 nm. 【Result and conclusion】The recoveries of 5 fluoroquinolones were 84.71%-99.66% at three spiked level(low, medium, high), and the relative standard deviations (RSD) were 0.20%-4.34%.The limit of quantiation (LOQ) of norfloxacin, ciprofloxacin and enrofloxacin was 10 μg·kg-1. The LOQ of danofloxacin was 2 μg·kg-1, and the sarafloxacin was 20 μg·kg-1. The method is simple, practical, and sensitive. It can meet the needs of determination of fluoroquinolones in chicken muscle.

    • A study on the distribution and dynamic changes of the glutathione peroxidase activity in pigs

      2014, 35(5):19-24. DOI: 10.7671/j.issn.1001-411X.2014.05.004

      Abstract (1428) HTML (0) PDF 604.11 K (1973) Comment (0) Favorites

      Abstract:【Objective】 To get a thorough understanding about the distribution and dynamic changes of the glutathione peroxidase (GPx) activity in pigs.【Method】 Using three varieties of pigs as the subject, GPx activities of different tissues and whole blood from pig with different species, genders, age periods and health statuses were determined in this study.【Result and conclusion】 The result showed that the GPx activity of whole blood from Lantang or Landrace pigs was slowly rising from 26 to 150 d, reaching a maximum at 150 d. The GPx activity of Lantang pigs was significantly higher than that of Landrace pigs during the periods of 30 and 90 d(P<0.05)and no significant difference in the other periods. The GPx activity of hybrid boars(Duroc×Yorkshire×Landrace) was significantly higher than that of sows(P<0.05); the GPx activity of healthy pigs (Duroc×Yorkshire×Landrace) was significantly higher than that of sick ones(P<0.05). Samples from different tissues revealed that kidney, spleen, liver, lung, heart and ileum showed a higher Gpx activity and these tissues can be used as ideal raw materials for extracting GPx.

    • >专题
    • Identification of polymorphism and function of LCYE allele in sweet corns

      2014, 35(5):25-30. DOI: 10.7671/j.issn.1001-411X.2014.05.005

      Abstract (2387) HTML (0) PDF 3.01 M (1529) Comment (0) Favorites

      Abstract:【Objective】The purpose of this investigation was to identify the polymorphisms of LCYE gene and the variation of β-carotene and carotenoids for understanding LCYE allele function in sweet corns and providing the reference and basis for biofortification breeding of pro-vitamin A.【Method】 The contents of β-carotene and total carotenoid were measured by HPLC in 47 sweet corn inbred lines. The whole length of LCYE was amplified, cloned and sequenced. Association analysis was performed by combining the sequences of LCYE gene with the content of β-carotene and total carotenoid.【Result and conclusion】 The total of 75 polymorphism sites were detected in this study, which included 49 polymorphism sites located on the non-coding chromosome region and 26 polymorphism sites located on the exons of the LCYE gene. All these sites on the exons were SNPs and most of them were located on the fifth exon except for the forth and the ninth exon with a conserved sequence. All 13 detected INDELs located on the non-coding region. The fragment of these INDELs ranged from 1 to 40 bp and most of them were from 4 bp to 8 bp. Three polymorphic sites were detected to be associated with β-carotenoid and 6 polymorphic sites were detected to be associated with total carotenoid. Exon 1-2 and exon 5-6 were found to be significantly associated with these two phonotypes. The result identifies that LCYE gene is related to the biosynthetic pathway of the carotenoids and provides the theoretical basis for biofortificated breeding of pro-vitamin A in sweet corns.

    • A genetic analysis and gene mapping of a new translucent and shrunken endosperm mutant in maize

      2014, 35(5):31-35. DOI: 10.7671/j.issn.1001-411X.2014.05.006

      Abstract (1969) HTML (0) PDF 2.12 M (2521) Comment (0) Favorites

      Abstract:【Objective】 To analyze the formation mechanism of translucent and shrunken maize endosperm.【Method】 The mutant M-E-479 of homozygous maize was used as experimental materials in our study. Through phenotypic identification, genetic analysis, main ingredients measurement and scanning electron microscopy, mapping population of M-E-479 was selected and map-based cloning technology was performed to locate the mutant gene.【Result and conclusion】 The result showed that the amount of fat increased, while the protein and starch decreased, and the starch grains changed in size, shape and space configuration in the mutant endosperm of M-E-479. The mutant gene is located between SSR markers bnlg1074 and umc1506 of chromosome 10 by using F2 population. Meanwhile, the genetic distance is about 3.6 cM and the physical distance is about 2.2 Mb. The results suggest that M-E-479 is a new translucent and shrunken maize endosperm mutant, and its mutant character may be controlled by a recessive single gene.

    • The response of maize root characteristics on population density

      2014, 35(5):36-41. DOI: 10.7671/j.issn.1001-411X.2014.05.007

      Abstract (1486) HTML (0) PDF 480.76 K (2016) Comment (0) Favorites

      Abstract:【Objective】 The response of maize root characteristics on population density were studied.【Method】 Four maize varieties Yifeng29, Zhengdan958, Xianyu335 and Xianyu420 were used as the tested materials. The number of brace root, dry mass and protective enzyme activities of root were measured, and the effects of population density on basic characteristics of root were determined.【Result and conclusion】 With the increase of population density, the number of brace root, dry mass and root activity increased first and then decreased, and the peaks occurred under different treatments. Protective enzyme activities of root were different with varieties, and MDA contents increased with the increase of population density. The percentage of lodging, the number of brace root and dry mass of root of Zhengdan958 were the biggest, while Xianyu335 had a higher protective activities.

    • A QTL analysis of main agronomic characters in rice,Oryza sativa

      2014, 35(5):42-51. DOI: 10.7671/j.issn.1001-411X.2014.05.008

      Abstract (1871) HTML (0) PDF 1.54 M (2707) Comment (0) Favorites

      Abstract:【Objective】 To identify excellent main effect QTLs conferring high yield, high quality in rice.【Method】 A genetic linkage map consisting of 142 simple sequence repeat (SSR) markers was constructed based on a recombinant inbred line (RIL) population derived from a cross between Japonica Zhongye 1211 and Indica Zhongda 304 in rice,Oryza sativa. Through an interval mapping method, a genome-wide quantitative trait loci (QTL) analysis was carried out for 13 agronomic traits of rice in the early and late seasons in 2012, including heading period, effective tillers per plant, plant height, panicle length, filled grains per plant, grains per panicle, seed setting rate, grain density per panicle, grain length, grain width, grain shape, 1000-grain mass, grain mass per plant.【Result and conclusion】 The number of QTLs related with the traits except that plant height was 2-15. A total of 73 QTLs for 12 traits were identified and mapped on all of the 12 rice chromosomes. Among all the 73 QTLs, there were 34 QTLs identified in the early season rice, 23 QTLs in the late season rice, and 16 QTLs in both of the early and late season rices. Each of the QTLs individually explained 5.3%-28.4% of the phenotypic variations, among which 10 QTLs were more than 20%. In both cropping seasons, many QTLs closely linked each other were detected in multiple sections of most chromosomes. Twelve new QTL loci are discovered, and 16 QTLs are identified in both cropping seasons can be used in marker-assistant-selected method.

    • Measurements of the capsaicinoid contents in Capsicum frutescens

      2014, 35(5):52-56. DOI: 10.7671/j.issn.1001-411X.2014.05.009

      Abstract (1998) HTML (0) PDF 399.73 K (3733) Comment (0) Favorites

      Abstract:【Objective】 The purpose of this study was to understand the pattern of capsaicinoid synthesis in Capsicum frutescens. 【Method】 With high performance liquid chromatography (HPLC), the placenta and pulp were sampled at 13 different periods after anthesis for analyses of capsaicin and dihydro capsaicin. All the data were subject to SAS software for multi-factor variance analysis.【Result and conclusion】 Capsaicinoids were synthesized in both the placenta and pulp. Their contents gradually increased to a peak and then slightly decreased. The contents of capsaicinoids were significantly affected (P=0.00≤0.01) by fruit tissues and developmental periods. The placenta had the highest content of capsaicinoids, which was 11.418 mg·g-1 at 21 d after anthesis.

    • Effects of different nutrient management models on the formation of yield, quality and nitrogen use efficiency of flue-cured tobacco

      2014, 35(5):57-63. DOI: 10.7671/j.issn.1001-411X.2014.05.010

      Abstract (1760) HTML (0) PDF 458.03 K (2134) Comment (0) Favorites

      Abstract:【Objective】 In order to solve the problems existing in the production of flue-cured tobacco about low nitrogen use efficiency (NUE) and inferior quality of flue-cured tobacco by unreasonable fertilizing technology.【Method】 A variety (Yunyan87) was used as a material in the field experiment. The technology of real time nitrogen management (RTNM) was applied in Meizhou. Two application modes of fertilizer (S40 and S43) based on SPAD(Soil and Plant Analyzer Development)value, the conventional application of fertilizer by farmer (CK2) and no fertilizer (CK1) were designed to study the influences of different application modes of fertilizer on the agronomic traits, nitrogen use efficiency (NUC), yield and quality. 【Result and conclusion】 The areas of the middle and upper leaves increased by adopting RTNM, and the other agronomic traits had no significant differences compared with CK2. The NUE was elevated obviously under RTNM, and the absorption of nitrogen of S40 and S43 increased by 48.74% and 45.42% compared with CK2, respectively. The yield and output value of tobacco leaf under RTNM increased more than those of CK2 and S43 achieved the highest yield and output value of tobacco leaf. Results indicated that, compared with the conventional application of fertilizer by farmer, RTNM could enhance high yield and output value of flue-cured tobacco and improve utilization rate of nitrogen fertilizer. Therefore, RTNM, especially based 43, is effective in practice and good for popularization at Meizhou Guangdong production area according the local condition.

    • >Plant protection
    • Sublethal effects of cyantraniliprole on the development and reproduction of the cabbage cutworm, Spodoptera litura

      2014, 35(5):64-68. DOI: 10.7671/j.issn.1001-411X.2014.05.011

      Abstract (1563) HTML (0) PDF 407.82 K (1808) Comment (0) Favorites

      Abstract:【Objective】 To study the effects of sublethal doses cyantraniliprole on the development and reproduction of Spodoptera litura in parental and F1 generations.【Method】 The sublethal doses including both LC20 and LC40 were estimated by the leaf-dipping method and the sublethal effects were investigated in parental and F1 generations under laboratory conditions.【Result and conclusion】 When the 3rd instar S. litura larvae were exposed to LC20 and LC40 of cyantraniliprole on an arum leave, Colocasia esculenta (L.) Schott for 72 h, the larvae duration from 3rd instar to 6th instar period increased by 2.39 and 4.32 d respectively, and the pupation rate (78.67%, 60.33%) and emergence rate (72.12%, 61.28%) were significantly lower than those of the control groups. Meanwhile, both the male and female pupal mass increased. Compared to the control groups, the laid eggs per female and the hatched rate significantly reduced in the LC40 group, but those did not occur in the LC20 group. No significant differences in pupal duration, sex ratio, and longevity of adults were observed in the treatment exposed to LC20 or LC40 dosages. The parent S. litura exposed to LC20 and LC40 doses of cyantraniliprole also extended the development duration in F1 generation larvae and reduced the pupation rate, with no significant influence on the emergence rate and the laying eggs per female. These results provide the theoretical basis for developing integrated pest management program for S. litura and the rational use of cyantraniliprole.

    • Effects of fungicides and bactericides and germicidal time on the growth of residual root of Pachira macrocarpa

      2014, 35(5):69-75. DOI: 10.7671/j.issn.1001-411X.2014.05.012

      Abstract (1660) HTML (0) PDF 440.34 K (2144) Comment (0) Favorites

      Abstract:【Objective】To screen the best fungicide and the best germicidal time of Pachira macrocarpa.【Method】 The effects of different fungicides and bactericides with different germicidal times on the residual root of reclaimed P. macrocarpa were investigated through pot experiments with peat soil. Five fungicides and bactericides (carbendazim, metalaxyl mancozeb, agricultural streptomycin, thiophanate methyl and potassium permanganate) were selected to study their effects on the shoot length,leaf mass,root length,root numbers,root mass,and root activities of the residual root of the reclaimed P. macrocarpa. The concentration of carbendazim, metalaxyl mancozeb, agricultural streptomycin, and thiophanate methyl was 1.25 g·L-1 while potassium permanganate was 2.0 g·L-1.【Result and conclusion】 The results showed that the best fungicide and bactericide was carbendazim, followed by thiophanate methyl, with the third being metalaxyl mancozeb and potassium permanganate and the least effective fungicide and bactericide being agricultural streptomycin. The preferred germicidal time was 0.5 and 1.0 h. The growth of the residual root of P. macrocarpa was significantly inhibited when it was disinfected over time(12 h).

    • Screening antagonistic microorganisms against ginseng diseases from Panax ginseng rhizosphere soil

      2014, 35(5):76-81. DOI: 10.7671/j.issn.1001-411X.2014.05.013

      Abstract (1501) HTML (0) PDF 1.19 M (2087) Comment (0) Favorites

      Abstract:【Objective】 To expand biocontrol strain resources and screen new strains with a higher antagonistic rate and a wider inhibiting range to control ginseng diseases.【Method】 The antagonistic strains were isolated by dilution plate method and were screened by flat-stand method.【Result and conclusion】 Seven actinomycetes strains with higher antagonistic activities to single ginseng pathogen, such asPhytophthora cactorum, Sclerotinia schinseng, Botrytis cinerea, Alternaria panax, Cylindrocarpon destructans, Rhizoctonia solani and Fusarium solani, had been screened, whose inhibition rates were 80.84%, 82.46%, 66.08%, 71.33%, 64.63%, 85.53% and 65.11%, respectively. Five strains, including a strain of Trichoderma and four strains of Actinomyces, had higher antagonistic activities to seven kinds of ginseng pathogens. The results indicate that the beneficial strains in the rhizosphere has a biocontrol potential ability of ginseng pathogens and can be used to prevent and cure major diseases of ginseng.

    • >Biological science
    • A correlation between ploidy levels and characters of leaf and flower in Phalaenopsis

      2014, 35(5):82-87. DOI: 10.7671/j.issn.1001-411X.2014.05.014

      Abstract (1847) HTML (0) PDF 1.33 M (3077) Comment (0) Favorites

      Abstract:【Objective】To research the correlation between ploidy levels and main characters in Phalaenopsis. 【Method】Ploidy levels of 85 Phalaenopsis accessions were identified by using squash method, and the length and density of stoma, the length, width and thickness of leaf, the diameter and the number of flowers were also measured. 【Result and conclusion】The results showed that in the 85 accessions there were three diploids (3.5%), nine triploids (10.6%) and 73 tetraploids (85.9%). Stomatal lengths and the length of tetraploid flower were obviously longer than those of diploid and triploid flower, while the density of stoma and the number of flowers were fewer than that of diploid and triploid(P<0.05). The differences of the stomatal cell length and diameter and the number of flower between diploid and triploid were not significant at 5% level, however, the stomatal density of triploid was less than that of diploid(P<0.05). The leaf length of tetraploid was significantly longer than that of diploid(P<0.05), while the difference of leaf length between diploid and triploid or between triploid and tetraploid was not significant(P>0.05). The difference of leaf width and thickness among diploid, triploid and teraploid were also not significant either(P>0.05).The ploidy levels were, to a large extant, positively associated to stomata length, leaf length and flower diameter, but negatively correlated with stomatal density and the number of flowers.

    • Molecular cloning, expression and bioinformatic analysis of TaLEA2 gene from wheat

      2014, 35(5):88-92. DOI: 10.7671/j.issn.1001-411X.2014.05.015

      Abstract (1853) HTML (0) PDF 426.92 K (2253) Comment (0) Favorites

      Abstract:【Objective】The cloning, expression and bioinformatic analysis of TaLEA2 gene were carried out to lay the theoretical basis for salt-tolerance of wheat.【Method】The TaLEA2 gene was obtained by homology cloning. Its gene structures and protein characteristics were analyzed by bioinformatic softwares. Real-time PCR was used to analyze its expression.【Result and conclusion】The results showed that TaLEA2 contained a 981 bp ORF encoding 326 amino acids. Using bioinformatics analysis to predict and analyze TaLEA2, the protein had classic domains and characteristics of LEA2, rich in Lys and lack of Cys, high hydrophilia (GRAVY, -0.405) and heat stability (Instability index, 25.28). The results of real-time PCR revealed that the expression of TaLEA2 had a constitutive expression, whose levels were different in different tissues. The expression was induced markedly by drought, salt, low-temperature and pathogenic bacteria in particular, but no change in treatment with ABA. Therefore,it is speculated that TaLEA2 can play a role not only in growth and development conditions but also in stress stage of wheat, especially in resistance to drought-stress.

    • Effects of 60Co-γ ray irradiation on the seed germination and seedling growth of Camellia semiserrata

      2014, 35(5):93-97. DOI: 10.7671/j.issn.1001-411X.2014.05.016

      Abstract (2056) HTML (0) PDF 591.42 K (1816) Comment (0) Favorites

      Abstract:【Objective】This study aimed to provide theoretical foundation and technical procedures of radiation mutation breeding for Camellia semiserrata and to improve the germplasm resource of this species. 【Method】Different doses of 60Co-γ ray irradiation were applied to the dry seed of this species, and the effects on seed germination and seedling growth were investigated.【Result and conclusion】Seed germination rate and seedling survival rate were reduced after irradiation treatment. Extremely significant positive correlation existed between irradiation and seedling mortality in the range of 10-90 Gy dose. The half lethal dose of radiation of the test samples was 47 Gy. The inhibition effect on seedling height and its ground diameter growth was found after different irradiations, and some seedlings produced a few branches. However, no obvious deformity in the seedling morphology is found.

    • Growth rhythm of Zenia insignis seedlings from four provenances

      2014, 35(5):98-101. DOI: 10.7671/j.issn.1001-411X.2014.05.017

      Abstract (1835) HTML (0) PDF 422.68 K (1620) Comment (0) Favorites

      Abstract:【Objective】 The purpose of this study was to understand the seedling growth rhythm of Zenia insignis from four different provenances.【Method】 The container seedling growth rhythm of Z. insignis was observed periodically. The growth curves of seedling height and ground diameter were built using the Logistic equation, and growth parameters were estimated.【Result and conclusion】 The result showed that the growth rhythm showed a “S” type curve. The determination coefficient varied from 0.954 to 0.983, and the fitting degree reached an extremely significant level. Therefore, seedling growth dynamics could be fitted by the Logistic equation. Different provenances showed different growth rhythms, but seedling growth of Z. insignis could be divided into 4 stages in general. The seedling germination stage was before March 1st; the early growth stage was from March 1st to April 30th in height, and from March 1st to April 9th in diameter; the fast-growing stage was from April 30th to September 20th in height, and from April 9th to October 12th in diameter; late growth stage was after September 20th in height and after October 12th in diameter. However, different provenances of Z. insignis showed differences in the starting and ending time and the duration of the 4 growth stages.

    • Wood anatomical characteristics of 17 Lauraceae species from southwest of Yunnan

      2014, 35(5):102-107. DOI: 10.7671/j.issn.1001-411X.2014.05.018

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      Abstract:【Objective】 The wood structures of 17 species belonging to Lauraceae from southwest of Yunnan were studied in order to provide a basis for wood identification, systematics, and taxonomy.【Method】 Wood samples were observed with the help of light microscope and scanning electron microscope.【Result and conclusion】 The results showed that the wood this studied had the following characteristics: The wood had diffuse porous with solitary pores and short radial multiple pores (2-3 cells), and few pore clusters for all the species. The vessels had two kinds of perforation plates: Only simple and both with simple and scalariform perforation and the number of scalariform perforation plate bars were from 3 to 14. The intervessel pitting was alternate. Wood rays were made up of few uniseriate and many multiseriate rays, and multiseriate rays often had 2-3 cells in the widest place. Most ray types were heteromorphosis Ⅱ and heteromorphosis Ⅲ, but few heteromorphosis Ⅰ existed as well. The types of vessel-ray pitting were almost large circular and reniform shape. Axial parenchyma was almost vasicentric and terminal parenchyma. Oil cells or mucous cells were widespread in ray and axial parenchyma cells. Wood classification table is made based on the observations in order to provide the basis for wood identification. The study of vessel perforation plates show that degree of evolution of wood belonging to Lauraceae is inconsistent, and Beilschmiedia and Cryptocarya are relatively evolutionary genera to some extent.

    • >Agricultural Engineering
    • A study of pass-ability of small multi-function chassis for intercropping in Southwest hills

      2014, 35(5):108-111. DOI: 10.7671/j.issn.1001-411X.2014.05.019

      Abstract (1435) HTML (0) PDF 690.23 K (1895) Comment (0) Favorites

      Abstract:【Objective】 To study pass-ability of small multi-function chassis for intercropping in Southwest hills.【Method】 The mathematical model was established by evaluating related geometric parameters of vehicle contour and analyzing the Southwest mountainous areas marked by intercropping cultivation agronomic angle and terrain features.【Result and conclusion】 Parametric test results showed that the theoretical and measured values were consistent. The highest vertical obstacles and the widest trench were 190 and 280 mm respectively. Chassis prototype climbing ability tests results showed that the multifunctional chassis prototype had a good climbing ability and it showed a good passing performance to climb under undulating ground conditions.

    • >Short communications
    • Application of Gibson Assembly method for plant expression vector construction

      2014, 35(5):112-116. DOI: 10.7671/j.issn.1001-411X.2014.05.020

      Abstract (8606) HTML (0) PDF 519.41 K (6655) Comment (0) Favorites

      Abstract:【Objective】 To construct a convenient and efficient plant expression vectors.【Method】 Gibson et al developed an isothermal in vitro recombination system or Gibson Assembly for ligation of multiple DNA fragments. The primers were designed to amplify target DNA sequences that contained 20-25 bp overlapping ends. The target DNA segments, linearized vector and the enzyme mixture were mixed in one tube to perform the assembly reaction.【Result and conclusion】 Using this method, several vectors for expressing rice genes were assembled. To increase the efficiency for cloning multiple fragments, the assembled primary products by PCR were amplified and then ligated into the vector. The Gibson Assembly method is faster and simpler without the limitation of internal restriction endonuclease sites of target genes, and it can be applied widely to construction of various vectors.