Abstract:【Objective】 To determine the apparent digestibility of dry matter, crude protein, crude lipid and crude fiber of dried or fermented brewer’s grains in grass carp (Ctenopharyngodon idellus), tilapia (Oreochromis niloticus), yellow catfish (Peltobagrus fulvidraco) and Scortum barcoo. 【Method】The experimental diets were consisted of 70% basal diet and 30% test feed ingredients, and Cr₂O₃ was added as an exogenous indicator. The fecal samples were collected by siphon after four weeks of feeding. 【Result】The apparent digestibility coefficients of dried and fermented brewer’s grains in different fish were significantly different (P<0.001). The apparent digestibility coefficients of dried and fermented brewer’s grains in grass carp and tilapia were significantly higher compared with those in yellow catfish and Scortum barcoo (P<0.05). After fermentation, the apparent digestibility coefficients of fermented brewer’s grain significantly increased compared to those of dried brewer’s grain in four fish species(P<0.05). 【Conclusion】 Herbivorous and omnivorous fish can utilize the dried brewer’s grain better compared with carnivorous fish. The utilization of dried brewer’s grain in fish can be improved by fermentation. Fermented brewer’s grain can be used in considerable amounts for herbivorous and omnivorous fish, but only in limited amounts for carnivorous fish.

Abstract:【Objective】 To investigate the mRNA expression of plasma membrane calcium channel, calcium-sensing receptor (CaSR) and adipogenic determination genes during the adipogenesis of porcine bone marrow mesenchymal stem cells (BMSCs).【Method】The porcine BMSCs, isolated and purified from 5-7 d piglet, were induced to adipogenic differentiation. Oil red O staining and triglyceride assay were used to detect the formation of adipocytes. The cells were collected at various time points (0, 1, 2, 5, and 10 d) during the adipogenic differentiation of porcine BMSCs. The mRNA expression patterns of zinc finger protein (Zfp423), preadipocyte factor 1 (Pref-1), bone morphogenetic protein 2 (BMP2), bone morphogenetic protein 4 (BMP4), plasma membrane calcium channel and CaSR genes were detected by real-time quantitative PCR. 【Result】The results of oil red O staining and triglyceride assay indicated that porcine BMSCs were successfully induced to adipogenic differentiation. The findings of real-time quantitative PCR demonstrated that the mRNA expression of Zfp423, Pref-1, BMP2 and BMP4 significantly increased at day 5(P<0.05), suggesting that day 5 is the key time for the adipogenic determination of porcine BMSCs. Meanwhile, the mRNA expression of voltage-dependent alpha-2/delta subunit 1 (CACNA2D1), calcium release-activated calcium channel modulator 1 (Orai1), transient receptor potential canonical type 1 (TRPC1), transient receptor potential melastatin 7 (TRPM7), transient receptor potential vanilloid receptor 1 (TRPV1) and CaSR also significantly increased at day 5, implying that calcium channels and CaSR gene might be involved in the adipogenesis of porcine BMSCs. 【Conclusion】Our data reveal the expression patterns of plasma membrane calcium channel, CaSR and adipogenic determination genes.

Abstract:【Objective】To explore the influence of G418 treatment of donor cells on the developmental efficiency of somatic cell nuclear transfer (SCNT) embryos.【Method】Porcine adult fibroblasts were collected after six days of G418 treatment. Expression levels of antioxidation and apoptosis-related genes were detected by quantitative RT-PCR before and after G418 treatment. The DNA methylation levels of LINE-1 repetitive sequences and microsatellites were analyzed using bisulfites sequencing analysis, and the in vitrodevelopmental rates of SCNT embryos were investigated.【Result】G418 treatment of donor cells caused significant (P<0.05) changes in expression levels of genes related to antioxidation and apoptosis, but did not significantly(P>0.05) change DNA methylation levels. SCNT embryos cloned from G418-treated donor cells exhibited a significantly (P<0.05) lower in vitro developmental rates compared with the control group.【Conclusion】G418 treatment of donor cell may inhibit the development of SCNT embryo.

Abstract:【Objective】In order to study the adaptabilities of introduced varieties of sheep and goot in Southern China. 【Method】 Hu sheep and Chuanzhong black goats were introduced from other provinces into Southern China. Their growth and reproduction performances and the relationship between growth, reproduction performances and ambient temperature and humidity were statistically analyzed.【Result】 Within one year of introduction, certain growth and reproduction performances of Hu sheep and Chuanzhong black goats declined in different degrees (P<0.01). Lambing percentage of Hu sheep was significantly correlated with ambient temperature and humidity (P<0.05), and the mortality rates of Hu sheep and Chuanzhong black goats were significantly correlated with temperature(P<0.05).【Conclusion】 There are adaptation problems of Hu sheep and Chuanzhong black goats within short period of introduction into Southern China from other provinces. The reproduction rate of female Hu sheep is negatively affected by the high temperature and humidity climate, and the monthly mortality rate of Chuanzhong black goat lambs largely rises in winter. Therefore, we should pay attention to the heatstroke prevention of Hu sheep in summer and heat preservation of Chuanzhong black goats in winter during management.

Abstract:【Objective】 To confirm genetic diversity distribution center of common wild rice，Oryza rufipogon Griff., in Guangxi, and construct the core collection which will provide a reference for protecting and utilizing wild rice resources in Guangxi. 【Method】 A total number of 623 common wild rice resources from Yujiang River Basin，Hongshui River Basin，Nanliujiang River Basin and Guibei Mountain were used to study the genetic diversity with 24 SSR markers. The stepwise cluster analysis was used to construct 10% and 5% core collections of common wild rice in Guangxi. 【Result】There were 114 alleles to be detected at 24 SSR loci. The number of alleles per locus (A) was 4.75, the average number of effective alleles (A_e) was 3.000 1, the Shannon-weaver information index (I) was 1.180 1 and the average expected heterozygosity (H_e) was 0.638 8. The order of genetic diversity of 9 regional populations was Yongning (YN) > Lingui (LG) > Fusui (FS) > Rongxian (RX) > Guigang (GG) > Pingnan (PN) > Gupeng (GP) > Wulitang (WLT) > Bobai (BB). The order of genetic diversity of four regions was Yujiang River Basin>Guibei Mountain > Nanliujiang River Basin > Hongshui River Basin. There were 31 copies belong to 5% core collection，including 14 copies of Yongning population and 12 copies of Fusui population which accounted for 5.76% and 18.75% of analyzed samples respectively. 【Conclusion】The genetic diversity center of Guangxi common wild rice is Yujiang River Basin. Wild rice of Yongning is widely distributed and presents various types. Wild rice of Fusui shows great rich genetic diversity. Yongning and Fusui regional populations，which are main sources of the core collection samples，are considered to be the most important populations of Guangxi common wild rice，and should be paid particular attention to their protection and exploitation.

Abstract:【Objective】To construct a plant expression vector including a lepidopteranresistant gene cry1Ab13 and use it to create a new transgenic maize germplasm with high resistance to Ostrinia furnacalis.【Method】The cry1Ab13 gene was inserted into expression plasmid pCAMBIA3300-Bar by homologous recombination method，and the plant expression vector pCAMBIA3300-cry1Ab13-Bar with Bar gene as a selection marker was obtained. Following Agrobacterium mediated transformation of the immature embryo of maize inbred line H99, the regenerated plants were detected by PCR and herbicide-resistance selection for each generation. T₂ generation plants were also detected by Southern blotting and real-time quantitative PCR. The resistance of transgenic plants to O. furnacalis was tested indoor and in the fields.【Result】A plant expression vector containing the cry1Ab13 gene was successfully constructed and three T₂ transgenic maize with high resistance and one T₂ transgenic maize with resistance to O. furnacalis were obtained. The cry1Ab13 gene was integrated into maize genome as confirmed by Southern blotting, and it was expressed in maize as shown by real-time quantitative PCR.The resistance of transgenic plants to O. furnacalis was improved significantly compared to non-transgenic control.【Conclusion】We introduced the cry1Ab13 gene into maize and its expression significantly improved the resistance of maize to O. furnacalis, which will promote the development of new insect-resistant maize germplasm.

Abstract:【Objective】 In order to determine the species and characteristics of phosphate solubilizing bacteria (PSB) in soybean, Glycine max, rhizosphere soil in Jilin Province.【Method】PSB were screened based on the formation of a halo zone of dissolving phosphate, and were identified by 16S rDNA gene sequencing and Vitek 2 system. Bacterial growth rates, soluble phosphorus concentrations, pH changes of the media and the types and amounts of secreted organic acids were determined. 【Result】WJ1, WJ3, WJ5 and WJ6 were isolated from soybean rhizosphere soil, and were identified as Pseudomonas sp., Enterobacter sp., Ochrobacterum sp. and Klebsiella sp. respectively. The maximum soluble phosphorus concentrations within 96 h of inoculating four PSB were 558, 478, 596 and 586 μg·mL^-1 respectively. The methyl red tests and pH measurements indicated that media pH all decreased during the phosphate solubilizing process of four PSB. Bacterial growth was limited when pH was below 4. Growth of WJ1 and WJ3 were slightly affected at pH 5, whereas WJ5 and WJ6 grew normally. Various organic compounds secreted from four PSB were analyzed by GC-MS, and among them α-ketoglutaric acid was identified as an abundant compound secreted by WJ1, WJ3 and WJ6.【Conclusion】All four PSB can effectively solubilize phosphate. The types of organic acids produced by the four PSB are not exactly the same，and the secretion of organic acids causes medium pH to decline, which inhibit the bacterial growth. The number of the bacteria determinines the amount of dissolved phosphorus.

Abstract:【Objective】 To build models for estimating chlorophyll a and chlorophyll b contents in potato，Solanum tuberosum， leaves by using hyperspectral vegetation indices, and provide a basis for scientific and nondestructive estimation of chlorophyll content in potato leaves. 【Method】Leaf spectral reflectance was measured for potato plants at different growth stages under different nitrogen levels by using a portable spectrometer. The hyperspectral vegetation indices were extracted from leaf spectral reflectance. The contents of chlorophyll a and chlorophyll b in potato leaves were measured, and the relationship between hyperspectral vegetation index and chlorophyll content was analyzed. 【Result】There were 12 vegetation indices with relatively good correlations with chlorophyll content. Modified normalized difference index(mND₇₀₅), modified simple ratio index(mSR₇₀₅),MERIS terrestrial chlorophyll index(MTCI) and modified chlorophyll absorption reflectance index(MCARI) had the highest correlations with chlorophyll contents among the 12 vegetation indices. Estimation models were established based on above-mentioned four indices. The power model based on MTCI offered the best estimation of chlorophyll a content, and the index model based on MCARI offered the best estimation of chlorophyll b content.【Conclusion】The power model based on MTCI and the index model based on mND₇₀₅ can be respectively used to estimate chlorophyll a and chlorophyll b contents in potato leaves with relatively high accuracy，and indirectly monitor nitrogen deficiency in potato plants.

Abstract:【Objective】To reveal the potassium (K⁺) adsorption properties of diatomite and tailings from Linjiang, Jilin Province, and provide theoretical basis for the development and utilization of diatomite and tailings as soil K⁺ regulation materials. 【Method】High quality diatomite raw ore and tailings were collected in Linjiang, their chemical and mineral compositions were analyzed, and their K⁺ adsorption and desorption characteristics were studied by static equilibrium adsorption method.【Result】The high quality diatomite and tailings were considerably different in their SiO₂ contents which were 78.57% and 89.29% respectively. Both were mainly composed of amorphous opal, yet only tailings contain some impurities such as quartz, kaolinite, mica and organic matter. The kinetic adsorptions of K⁺ by both high quality diatomite and its tailings were in accordance with pseudo two order kinetics model. The thermodynamic adsorptions of K⁺ by both were consistent with the Freundlich isotherm adsorption equation, and the adsorption reactions were easy to carry out. The K⁺ desorptions by both were consistent with the Elovich equation. The K⁺ desorption rates and the logarithmic values of reaction time (Lnt) were well linearly correlated before the desorption process reached equilibrium for both the high quality diatomite and tailings. 【Conclusion】 Diatomite tailings are mainly composed of amorphous opal, and has certain capacity of K⁺ adsorption and desorption.

Abstract:【Objective】 To study the effects of temperature and host on Cacopsylla citrisuga growth and development.【Method】C. citrisuga was parasitized on healthy or Candidatus Liberibacter asiaticus-infected Citrus limon in the incubators under simulative natural environment. The developmental periods, survival rates of different life stages, preoviposition period and fecundity of C. citrisuga were analyzed and compared. Adult survival rates were compared for C. citrisuga parasitized on C. limon, Murraya exotica and Citrus sunki under 15, 20 and 25°C.【Result】Under 15 °C, 70% relative humidity and on healthy C. limon, the mean duration of C. citrisuga egg was 9.38 days, the durations from 1st to 5th instar nymph were 8.80, 6.66, 6.00, 6.76 and 5.94 days respectively, the preoviposition period was 16.2 days, the total duration of generation was 59.74 days and the survival rate of generation was 28.34%. For C. citrisuga parasitized on Ca. L. Asiaticus-infected C. limon, the durations of 2nd instar, 4th instar and total nymph stages significantly decreased, the preoviposition period significantly increased, and the survival rates of 4th instar, total nymph stages and generational period significantly decreased compared to those on healthy lemon. There was no significant difference in fecundity when the same origin psyllid females were laying eggs on healthy and infected C. limon but the fecundity of the subsequent generation females emerged from healthy C. limon was significantly higher compared to those from infected C. limon. The survival rate of C. citrisuga adults was the highest on C. limon comparing three hosts under the same temperature, and was the highest under 15°C comparing different temperatures when reared on the same host plant species. There were 81.67% adults survived after 20 days of being parasitized on C. limon under 15 °C. The nymph developed faster when parasitized on Ca. L. Asiaticus-infected C. limon, but the pathogen negatively influenced the survival and reproduction of C. citrisuga. 【Conclusion】C. citrisuga can accomplish the whole generation with high survival rate under the above conditions, which can be applied to breed C. citrisuga indoor, and can be used for other studies related to C. citrisuga growth and Huanglongbing mechanisms.

Abstract:【Objective】To screen entomopathogenic fungal strains with high virulence against Asia citrus psyllid (ACP), Diaphorina citri Kuwayama, and to provide a basis for development of microbial pesticide against ACP. 【Method】The growth and sporulation rates of five Beauveria bassiana strains numbered A to E and five Isaria fumosorosea strains numbered A to E, and the mortality rates of ACP after infected by these ten fungal strains were investigated. 【Result】 The spores quantity of five B. bsssiana strains were significantly different after cultured for 10 days, strain E had the largest spores quantity(3.78×10⁸ mL^-1) and strain A had the least spores quantity(7.96×10⁷ mL^-1). There was significant difference of spores quantity between strain E and strain A. There was no significant difference among the mortality rates of 2-3 instar ACP nymphs seven days after the spray of 1×10⁶ mL^-1 B. bsssiana fungal spores, and the highest mortality rate (78.0%) was found in the strain A treatment, followed by the strain E treatment (77.0%). For five I. fumosorosea strains, the spores quantity after cultured for 10 days, strain B had the highest sporulation rate (6.04×10⁸ mL^-1) and strain E had the least spores quantity(6.28×10⁷ mL^-1). There was significant difference of spores quantity between strain B and strain E. The mortality rates of 2-3 instar ACP nymphs seven days after the spray of 1×10⁶ mL^-1I. fumosorosea fungal spores were higher than the control group except strain D. ACP infected by strain B had the highest mortality rate(81.0%). 【Conclusion】B. bsssiana strain E and I. fumosorosea strain B are considered with high virulence against ACP and can be potentially used for biological control of ACP.

Abstract:【Objective】To investigate rules of heartwood formation and essential oil distribution of Indian sandalwood, Santalum album, plantation in Hainan, and provide reference for development of sandal plantation in large scale in China. 【Method】The presence of heartwood, heartwood proportion, and the distribution of essential oil content and composition of 21-year-old sandal plantation in Jianfeng mountain, Hainan were surveyed by random sampling. 【Result】The heartwood had formed naturally in all sampled sandals. The average heartwood diameter, heartwood proportion and essential oil content at 30 cm above ground were 10.14 cm, 38.01% and 4.36%, respectively. There was positive linear correlation between ground diameter and heartwood diameter (r=0.873), but no clear correlation was found between ground diameter and heartwood proportion (r=0.279). The distribution of essential oil was not significantly different among different azimuths including east, south, west and north（P=0.197）,but was significantly different among different radial positions (P<0.05). The essential oil content in sapwood was significantly lower than that in heartwood. Totally 56 constitutes were isolated from the essential oil, of which 42 were identified. Although the number of constitutes in the essential oil extracted from heartwood was less than that from sapwood, the oil quality of heartwood met the ISO standard (ISO3518:2002) for S. album oil while the oil quality of sapwood did not. 【Conclusion】Hainan is considered as a suitable region for sandal growth given the good growth performance, high heartwood proportion and essential oil content.

Abstract:【Objective】To analyze and identify the chemical compositions of volatile oils extracted from Taxodium ascendens leaves and cones, and test the inhibition activities against seven different bacteria.【Method】The volatile oils were extracted by hydro-distillation from the leaves and cones of Taxodium ascendens and the chemical compositions of the essential oils were analyzed by GC-MS. The inhibitory activities against bacteria were tested using the filter paper diffusion method. 【Result】The yield rates for the essential oils of fresh leaves and cones were 0.211% (w) and 0.657% (w), respectively. Totally 21 components were identified from the volatile oil of leaves, which accounted for 90.410% of the total volatile content. (1R)-α-pinene (70.149%), (-)-α-terpineol (7.072%), 4-carene (2.025%) and β-pinene(2.012%) were the major compounds in the volatile oil of leaves. Totally 13 components were identified from the volatile oil of cones, which accounted for 95.285% of the total volatile content. (1R)-α-pinene (78.609%), ferruginol (4.276%), 4-carene (3.355%), limonene (2.324%) and β-terpinene (2.179%) were the major compounds in the volatile oil of cones. There were only four common components in the volatile oils of both leaves and cones including (1R)-α-pinene, 4-carene, limonene and borneol. The volatile oil of cones showed the strongest inhibitory activity against Agrobacterium tumefaciens and the inhibition zone diameter was （34.5±2.3） mm, while the volatile oil of leaves showed the strongest inhibitory activity against Staphylococcus haemolyticus and the inhibition zone diameter was （16.0±1.2） mm.【Conclusion】The content of volatile oil in T. ascendens cones is higher than that in leaves, and (1R)-α-pinene is the main ingredient in both. The volatile oil of cones has higher antibacterial activity than that of leaves.

Abstract:【Objective】To determine the effect of 20 different fungi on compositions of essential oils from agarwood of Aquilaria sinensis.【Method】The whole-tree agarwood-inducing technique was used, and culture fluids of 20 fungal species were injected into A. sinensis trees separately. The chemical compositions of extracts from the 20 treatments were investigated using GC-MS method.【Result】The ethanol extract contents were above 10% from A. sinensis trees infected by seven fungal species including Lasiodiplodia theobromae,Penicillium meleagrinum, Trichoderma atroviride, T. koningiopsis, Pen. italicum, Fusarium solani and Botryosphaeria rhodina. The total relative contents of sesquiterpenes, aromatics compounds and 2-(2-phenylethyl) chromone derivatives were 21.57%, 21.85%, 24.02%, 22.83%, 23.49%, 25.15% and 24.84% respectively. For the rest 13 treatments, the ethanol extract contents were below 10%, the main detected ingredients were fatty acids and alkanes，the total relative contents of sesquiterpenes and aromatics compounds were only 2.67% to 7.88%, and no 2-(2-phenylethyl) chromone derivative was detected.【Conclusion】Among the 20 fungal species, seven species can efficiently induce agarwood formation in A. sinensis, and the effects of the rest 13 species are not obvious.

Abstract:【Objective】To find out the growth rhythms of the seedlings of Chinese unique precious fast-growing timber species Toona sinensis, for raising seedling quality and tending effect of young stand.【Method】The seeds were collected from 14 representative locations of T. sinensis distribution area. Seedling growth was observed continuously for one year. Logistic equations were used to fit the growth in seedling height and ground diameter, and seedling growth stages were divided.【Result】The growth in seedling height of different provenances had three stages. Slow growth period was from January to April. Rapid growth period was from mid-May to the end of September, followed with another slow growth period. The "Slow-Fast-Slow" growth process showed "S" type rhythm. The growth rhythm of the ground diameter also showed as "S" type, whereas the beginning time of rapid growth period was generally earlier than that of height growth, and the duration of rapid growth was generally longer by continuing until mid-or late October. There were significant differences in growth rhythms among seedlings of different provenances. The rapid growth period of southern provenances was from April to September, lasting for a long time. The rapid growth period of northern provenances was relatively short and was mainly in June and July. The ground diameters of southern provenances continued to grow fast in August and September. However, the northern provenances step into a slow growth period in July or August, and in general stopped growing after mid-September.【Conclusion】Northern provenances are not suitable for cultivation in Guangdong. There are high precisions in the logistic equations used to fit growth in height and ground diameter for seedlings of different provenance. According to the seedling growth curves, the growth process of T. sinensis provenances can be divided into three stages. Rapid growth stage is the key time for seedling growth. During this stage the management of water and fertilizer should be reinforced to promote T. sinensis growth.

Abstract:【Objective】To utilize the superior properties of mature Acacia auriculiformis, improve propagation efficiency, and accelerate breeding and promotion of elite varieties.【Method】A. auriculiformis bud inducing system was established by comparing the disinfection strategies for the explants from 16-year-old A. auriculiformis and screening the bud inducing media. The proliferation media were screened and the relationship between subculture times and proliferation rate was studied. 【Result】 The optimal explants were those collected from the 3th-5th node of shoots in August. For the optimal explants treated with 0.1% mercuric chloride for 18 min and 75% ethanol for 15 s, and inoculated onto the best bud inducing medium containing MS medium + 6-BA 1.0 mg·L^-1+sucrose 40 g·L^-1, the germination rate reached 92.00%. When type II medium (MS+ 6-BA 1.0 mg·L^-1+ NAA 0.1 mg·L^-1+Ac 0.05 g·L^-1+sucrose 30 g·L^-1) was used for proliferation, the proliferation rate was high and stable with an average rate of 2.63 for seven generations. 【Conclusion】 This tissue culture system enables fast propagation for mature elite plants of A. auriculiformis.

Abstract:【Objective】To study internal stress variations of citrus fruits clamped by working robot under different clamping conditions, and to predict and analyze clamping damages on citrus fruits. 【Method】The mechanical parameters of citrus peel and pulp were measured. Finite element model of each tissue structure of citrus fruit was established. The processes of clamping citrus fruits by robot with different finger planes were simulated. The verification experiments of citrus fruit clamping were performed using a custom terminal clamping platform.【Result】The elastic modulus of citrus peel and pulp under vertical stress were 11.408 and 0.277 MPa respectively, and the limiting stress of citrus peel and pulp were 1.250 and 0.048 MPa respectively. The equivalent stress distribution diagrams of citrus peel and pulp were obtained in the simulation tests. The stress of peel was lower than that of pulp. Because the limiting stress of pulp was lower than that of peel，the pulp started to damage before the peel did. The stress on both peel and pulp caused by arc fingers was lower than that caused by plane fingers under the same clamping force. The peel started to damage at 23 N clamping force for the plane fingers, while the damage initiated at 45 N clamping force for the arc fingers. At the clamping force of 12, 23, 34, 45, 56 and 67 N，the pulp damage rates for the plane fingers were 0, 10%, 30%, 40%,20% and 20% respectively higher than those for the arc fingers. The results of verification experiments using the clamping platform were consistent with the simulation outcomes. 【Conclusion】 The clamping damage on citrus fruit can be predicted and evaluated. This study provides a basis for designing damage reducing structures in citrus picking robot.

Abstract:【Objective】To explore the relationship between CO₂ filling amount and the performances of the heat pump dryer system including the system power, the drying chamber temperature and the system performance coefficient, and to determine the optimal CO₂ filling amount.【Method】The working principles of the heat pump dryer were analyzed according to the actual production requirements. The CO₂ heat pump drying experimental platform was established. Data collection and monitoring system design were conducted by PLC. CO₂ filling experiments were performed.【Result】When the CO₂ filling amount was 2.5 kg, the system power reached 1 200 W, the drying chamber temperature reached 70 ℃, the energy consumption rate （C_OP） was 3.89, the dehumidification rate was 1.636 kg·h^-1, the pressures of the cooler and evaporator were suitable, and the temperature difference between inlet and outlet was relatively large.【Conclusion】This system has high energy efficiency and excellent performance.

Abstract:【Objective】To optimize the structure parameters of cell wheel notoginseng precision seeding apparatus and improve seeding performance. 【Method】 Notoginseng seeds were chosen as materials. Number, diameter and depth of type hole, along with cell wheel rotational speed of seed metering device were selected as four influence factors to carry out quadratic orthogonal rotation combination test. Seeding qualified index, repeat sowing index and leakage sowing index were tested by the self designed soil bin test bed. The test results were analyzed by response surface methodology, and quadratic regression models between various factors and test indexes were established. The influence rules of various factors, interaction terms and quadratic terms on the seeding qualified index were studied. Test factors were optimized and verified by actual experiment. 【Result】 The order of factors affecting the seeding qualified index was diameter of type hole > depth of type hole > number of type hole > rotational speed of cell wheel. The seeding qualified index was above 90, the repeat sowing index and leakage sowing index were below 5 when the depth of type hole was 5.4 mm, the rotational speed of cell wheel was 35 r·min^-1, the number of type hole was 12-14 and the diameter of type hole was 7.7-8.2 mm. Factors were verified after optimization, and the experimental results were generally in agreement with the optimization results. 【Conclusion】 The performance of this seeding apparatus can be optimized by response surface methodology. This research provides a theoretical basis for designing notoginseng seeder and mechanized sowing of notoginseng seeds.