ZOU Shuzhan , LI Bing , CHEN Anni , WU Jiajun , JIANG Zeng , LUO Manlin
2018, 39(4):1-6. DOI: 10.7671/j.issn.1001-411X.2018.04.001
Abstract:【Objective】To evaluate the immunopotentiation effects of levamisole hydrochloride (LH), concanavalin A (ConA) and muramyl dipeptide (MDP) on pseudorabies virus (PRV) inactivated vaccine.【Method】The immunopotentiators were mixed into inactivated PRV virus aqueous phase, and emulsified with oil phase to make inactivated vaccines containing immunopotentiators. The final concentrations of immunopotentiators were LH 7.5 mg·mL-1, ConA 25.0 mg·mL-1 and MDP 150.0 mg·mL-1 respectively. The second immunization was on the 14th day after the first immunization. Mouse serum samples were collected on the 14th day after the first immunization, 7th and 14th day after the second immunization to detect relative cytokines (IL-2, IL-4, IFN-γ) and specific antibody levels. On the 14th day after the second immunization, all mice were attacked and the final attack protective rate was calculated.【Result】Compared to Baiyou group, ConA and MDP groups extremely significantly improved IL-2 content in mouse serum on the 14th day after the second immunization. LH group significantly improved IL-4 content on the 14th day after the first immunization, and MDP group significantly improved IL-4 content on the 14th day after the second immunization. The three immunopotentiators extremely significantly enhanced IFN-γ contents and specific antibody levels on the 14th and 7th day after the second immunization respectively, and also improved the attack protective rates. The survival rates of LH and ConA groups were 20%, while that of MDP group was 60%.【Conclusion】Three immunopotentiators within the range of the experimental dosages can enhance the immune effects of PRV inactivated vaccine in varying degrees.
TENG Jinyan , WANG Jiaying , ZHANG Jing , XU Yunxin , JI Congliang , ZHANG Xiquan , ZHANG Zhe
2018, 39(4):7-12. DOI: 10.7671/j.issn.1001-411X.2018.04.002
Abstract:【Objective】To investigate the effects of feeding behavior on production performance of broiler chickens, and provide references for chicken management.【Method】The automatic feeding system was used to collect the data of feeding behavior and production performance for 45-day-old yellow-feather broiler chickens. The change of feeding behavior for chickens over 24 hours was summarized. The correlation between feeding behavior and production performance was analyzed.【Result】There were two peaks of feeding in one day including the times from 06:00 to 10:00 and from 17:00 to 19:00. The average feeding speed per chicken of the flock was higher in the early or late time of the feeding peaks and the highest feeding speed was about 3.0 g·min-1. The correlation between single feeding behaviors (single feed intake, single feeding duration, single feeding speed) and production performances (average daily feed intake, average daily gain, feed conversation ratio) were significant. The average number of daily meals was significantly negatively correlated with average daily gain, while positively correlated with feed conversation ratio. The average duration of daily feeding time was not significantly correlated with production performance.【Conclusion】There is a close relationship between single feeding behavior and production performance of chickens. The production performance can be increased by selecting chickens having less number of daily meals.
CAO Shiqi , GENG Yi , YU Zehui , ZHENG Liping , LEI Xueping , CHEN Defang , OUYANG Ping , HUANG Xiaoli , HUANG Yanzhen
2018, 39(4):13-19. DOI: 10.7671/j.issn.1001-411X.2018.04.003
Abstract:【Objective】To explore the etiology of a serious infectious disease occured in hybrid sturgeon (Acipenser schrencki ♀×A. baeri ♂) from a farm of Qionglai, Sichuan Province.【Method】The pathogens were isolated from the liver, spleen and kidney tissues of diseased fish, and identified by physicochemical characteristics, 16S rRNA and gyrB gene sequences analyses, meanwhile the susceptibility test and histopathological observation were performed.【Result】One gram-negative predominant bacteria strain (YC160412) was isolated and identified as Yersinia ruckeri by physicochemical characteristics, 16S rRNA and gyrB gene sequences analyses. The antibiotic susceptibility test showed that the pathogen was sensitive to cefazolin, ampicillin, florfenicol, and resistant to amoxicillin and erythromycin. Histopathological observation found that Y. ruckeri infection caused significant damages to hybrid sturgeon multiple organs, especially in liver, spleen, kidney, intestine and gill. The main pathologic lesions were obvious degeneration, necrosis, hemorrhage and infiltration of the inflammation cells. The pathogen was largely found in liver, spleen and kidney tissues.【Conclusion】This study confirms Y. ruckeri is the pathogen of the epidemic. Its infection can cause multiple organ dysfunctions and even death of hybrid sturgeon.
ZHOU Yaojia , TU Zunfang , SHUI Fei , YANG Ruixue , WANG Kaiyu , GENG Yi , HUANG Xiaoli , OUYANG Ping
2018, 39(4):20-24. DOI: 10.7671/j.issn.1001-411X.2018.04.004
Abstract:【Objective】To confirm the effect of temperature on proliferation in vitro and pathogenicity of Cyprinid herpesvirus 3 (CyHV-3).【Method】CyHV-3-EGFP strain was used in this study. Viral titer was measured by the indirect immunofluorescence staining assay. The proliferation of virus in vitro was observed using a fluorescence microscope. The thermal stability, and the effect of temperature on proliferation in vitro and pathogenicity in vivo of virus were studied.【Result】CyHV-3 had good thermal stability at 4-36 ℃. The viability of CyHV-3 decreased with the increase of temperature when the temperature exceeded 36 ℃. The virus viability was completely lost at 50 ℃. The infection temperature had little effect on the viability of CyHV-3, and the virus showed infectivity at both high (37 ℃) and low (4 ℃) temperatures. However, incubation temperature significantly affected the virus viability. CyHV-3 proliferation capacity decreased or even disappeared when the temperature exceeded 30 ℃. After CyHV-3 infection, the mortality rates for the carps at low temperature (15 ℃) and room temperature (25 ℃) were 26.67% and 73.33% respectively, and the mortality rates for the carps at high temperature (30 ℃) and in the control group with no virus infection were 0.【Conclusion】The results confirm that CyHV-3 can proliferate and has pathogenicity at low temperature, which explain the phenomenon that the outbreak and rapid spread of KHVD in recent years are no longer restricted in spring and autumn seasons.
LI Qi , WU Zaijing , LIN Fangyuan , HU Fei
2018, 39(4):25-32. DOI: 10.7671/j.issn.1001-411X.2018.04.005
Abstract:【Objective】To investigate the relationships of the yields, qualities and heavy metals contents of allelopathic rice with water managements and planting densities, and make better use of environmentally friendly characteristics of allelopathic rice.【Method】The rice materials, including ‘Allelopathy rice No.3’, ‘Hualiangyou 78’, ‘Yuxiangyouzhan’ in early season and ‘Baixiangzhan’ in late season, were cultivated under two different water managements (WN: paddy field drainage during booting stage, WS: paddy field continuous irrigation during booting stage) and two different planting densities (DH: high planting density, DN: local planting density). And then their yields, qualities, and Pb and Cd contents in grains were investigated.【Result】The rice yields and qualities had no significant differences in different water and density treatments, but differed among rice cultivars. ‘Hualiangyou 78’ yield was the highest in all materials. WSDN treatment significantly reduced the milled rice percentage of ‘Baixiangzhan’, but had no significant effect on allelopathic rice cultivars. WS treatment reduced the contents of Pb and Cd in rice grains, especially the contents of Cd in allelopathic rice cultivars. The planting densities and rice cultivars had no significant effect on Pb and Cd contents in rice grains.【Conclusion】Both water and density treatments have no significant effect on yields and qualities of allelopathic rice materials, but WS can significantly reduce the Cd contents of allelopathic rice grains.
DENG Jie , DAI Linjian , SU Zhaohong , WANG Jianbo , YANG Su , ZHOU Tian
2018, 39(4):33-38. DOI: 10.7671/j.issn.1001-411X.2018.04.006
Abstract:【Objective】To explore the effect of TiO2 spraying agent on photosynthesis and chlorophyll fluorescence parameters of upper tobacco leaves, and provide a reference for improving the availability of upper tobacco leaves.【Method】TiO2 spraying agent was preparated by means of sol-gel method. The effects of different concentrations and formulas of TiO2 spraying agent on photosynthetic pigment content, photosynthetic physiological indexes and chlorophyll fluorescence parameters of upper tobacco leaves at maturity were studied.【Result】TiO2 spraying agents retarded the degradation rates of chlorophyll and carotenoid of upper tobacco leaves. The spraying agents reduced photosynthetic rate, stomatal conductance and transpiration rate of upper tobacco leaves, increased the intercellular CO2 concentration in upper tobacco leaves, but leaf temperature was unaffected. Photosynthesis and chlorophyll fluorescence parameters of upper tobacco leaves were significantly affected by TiO2 spraying agents. Compared with the spraying water control, the spraying agents increased Fv/Fm, Fv/Fo and qP, and decreased Fo and NPQ. The absorption efficiency of light intensity of tobacco leaves sprayed titanium dioxide or titanium dioxide composite graphene was the best at the concentration of 0.45 g·L-1.【Conclusion】Tobacco leaves can absorb ultraviolet (UV) to a certain extent, and the efficiency of spraying titanium dioxide composite graphene was better. There was a threshold of spraying agent concentration for light absorption efficiency.
ZHANG Yali , XIAO Jinqing , MA Ruijun , HUANG Qian , CHEN Yu , ZHENG Pufeng , QIU Zhi
2018, 39(4):39-45. DOI: 10.7671/j.issn.1001-411X.2018.04.007
Abstract:【Objective】The soil magnetic susceptibility is a common parameter for monitoring environmental pollution, and usually measured on the surface of bare soil. The farmland soil is often covered with crop straw, and this paper mainly studies the influence of straw mulching on farmland soil magnetic susceptibility measurement.【Method】The measuring points were set in paddy field, sugarcane field 1, sugarcane field 2 and corn field respectively, and piled up with straws artificially and evenly. The soil magnetic susceptibility values were measured under different straw materials and straw mulching thicknesses to analyze the influence of straw mulching on farmland soil magnetic susceptibility measurement.【Result】The soil magnetic susceptibilities decreased constantly with the increase of straw mulching thickness. When the thicknesses ranged from 0 to 5 cm, soil magnetic susceptibilities attenuated rapidly, but the values could reflect the magnetic susceptibility of bare soil to a certain extent. When the thicknesses exceeded 6 cm, soil magnetic susceptibilities attenuated slowly and when the thicknesses exceeded 10 cm, soil magnetic susceptibilities tended to zero. The test data of most measuring points were fitted with index model, while that of a few points were fitted with linear model. Data of all measuring points in paddy field, sugarcane field 1, sugarcane field 2 and corn field were respectively integrated, and the relationships between soil magnetic susceptibility and straw mulching thickness in above fields were characterized by index, index, index and linear negative correlations. The corresponding detection distances of different straw mulching thicknesses were the main factors causing the attenuation of soil magnetization susceptibilities, and the influences of straw materials on the measured value were very small.【Conclusion】When measuring soil magnetic susceptibility with MS2D magnetic susceptibility meter, it is best to measure bare soil directly. If there is straw mulching, the mulching thickness should not exceed 5 cm. The influence of straw mulching thickness on soil magnetic susceptibility can be characterized by index function or linear negative correction model.
ZHOU Xiazhi , LANG Kun , ZHANG Shuping , YU Yan , LI Shang , WANG Zhenxing , BI Shoudong , ZOU Yunding , WANG Jianpan , LIU Feifei
2018, 39(4):46-54. DOI: 10.7671/j.issn.1001-411X.2018.04.008
Abstract:【Objective】To clarify the relationships of Stephanitis chinensis and Lygus lucorum with natural enemies in ‘Baihaozao’ tea garden.【Method】The population numbers of pests and natural enemies were investigated. The numerical, temporal and spatial relationships of natural enemies with S. chinensis and L. lucorum were studied using grey relational analysis and niche analysis.【Result】The major natural enemies of pests in ‘Baihaozao’ tea garden included Tetragnatha squamata, Tetragnatha maxillosa, Theridion octomaculatum, Clubiona japonicola, Neoscona theisi, Misumenops tricuspidatus, Erigonidium graminicolum and Oxyopes sertatus. The top four enemies showing close relationships with S. chinensis were C. japonicola, O. sertatus, T. maxillosa and N. theisi in 2015, while C. japonicola, O. sertatus, E. graminicolum and N. theisi in 2016. The natural enemies ranking the first, second and fourth were the same in two years. The differential degree between the ranks of natural enemies in two years was 62.5%. The top four enemies showing close relationships with L. lucorum were T. maxillosa, M. tricuspidatus, T. squamata and T. octomaculatum in 2015, while M. tricuspidatus, N. theisi, T. maxillosa and E. graminicolum in 2016. Two of the four major enemies were the same in two years, but the order was different. The differential degree between the ranks of natural enemies in two years was 87.5%.【Conclusion】The changes in ranks of natural enemies possibly depend on the ratio of the number of pests to their natural enemies.
ZHONG Weixin , SHI Keyi , CHEN Yiqun , WANG Xiaopei , TAO Wanyu , MA Kaixiong , SUN Yongxue
2018, 39(4):55-60. DOI: 10.7671/j.issn.1001-411X.2018.04.009
Abstract:【Objective】To investigate the prevalence of antibiotic resistant genes of endophytic bacteria in cherry tomatoes sold in Guangzhou, and discuss the possibilities of resistant genes transferring into cherry tomatoes based on GFP-tagged Escherichia coli artificial plantation model.【Method】The endophytic bacteria in commercially available cherry tomatoes was identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Polymerase chain reaction was used to qualitatively detect the prevalence of resistant genes including chloromycetin (cmlA), tetracycline (tetA and tetM), sulfonamide (sul1, sul2 and sul3) and quinolones (oqxA, oqxB, qnrB, qnrS and qepA). The GFP-tagged E. coli artificial plantation model was used to detect target GFP-bacteria in cherry tomato fruits, leaves and roots, and explore the possibilities of entophytic bacteria and GFP tag transferring into cherry tomatoes.【Result】 Enterobacter and Klebsiella accounted for a relatively high proportion in 52 endophytic bacterial strains, and almost all strains carried oqxB gene with a total positive rate of 92.31%. The endophytic bacterial strain containing GFP tag was isolated from the fruit collected on the 27th day in artificial plantation model.【Conclusion】oqxB gene is most prevalent in cherry tomato endophytic bacteria. The antibiotic resistant genes can transfer into cherry tomato fruits through root irrigation.
WANG Hua , WANG Wangwei , LIU Chunyi , WANG Qiaoqiao , ZHU Liwu , XU Xiaoniu
2018, 39(4):61-67. DOI: 10.7671/j.issn.1001-411X.2018.04.010
Abstract:【Object】To discover key genes related to drought resistance of pear plants based on transcriptome data, and provide a theoretical foundation for the breeding of drought resistant pear varieties.【Method】High through-put transcriptome sequencing by illumina Hi Seq TM 2000 was performed using leaves of ‘Huangguan’ pear (Pyrus bretschneideri ‘Xuehuali’×P. pyrifolia ‘Shinsseiki’) and ‘Hwangkumbae’ pear (P. pyrifolia ‘Niitaka’×P. pyrifolia ‘Nijisseiki’) under normal irrigation and drought stress treatments. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) databases were used to analyze the differentially expressed genes (DEGs) in two pear genotypes.【Result】There were 4 377 and 3 841 DEGs comparing two pear genotypes under control irrigation and drought stress, respectively. Among these DEGs, 1 340 DEGs were only found under drought stress. There were 1 387, 922 and 1 235 DEGs in three ontologies including biological process, molecular function and cellular component, respectively. There were 349, 139 and 151 DEGs enriched in terms related to drought response of plant including metabolic process, stress response and biomembrane, respectively. The 1 340 DEGs only found under drought stress were matched to 102 KEGG pathways, and three of the pathways were related to phytohormone biosynthesis. Furthermore, among the 1 340 DEGs, 37 genes were annotated as transcription factors which belonged to 17 transcription factor families, and the ethylene responsive factor (ERF) family had 11 DEGs.【Conclusion】In this study, some genes related to endogenous hormone metabolism and transcription factors are found to express differentially in two pear genotypes under drought stress. These genes are probably closely related to the genotypic differences in drought resistance. Our results provide a basis for future study of the molecular mechanism of drought resistance of pear plant.
LIU Xiaozhou , FAN Yanping , YU Rangcai , YUE Yuechong , LI Xinyue , YU Yunyi
2018, 39(4):68-72. DOI: 10.7671/j.issn.1001-411X.2018.04.011
Abstract:【Objective】To investigate the effect of ethylene and 1-methylcyclopropene (1-MCP) treatment on the terpenoid fragrance of Hedychium coronarium and analyze the expressions of related genes.【Method】H. coronarium was treated separately with 10 μL·L-1 ethylene and 4 μL·L-1 ethylene inhibitor 1-MCP for 8 h. Volatile scent compounds were measured by headspace solid-phase microextraction and gas chromatography-mass spectrometry (GC-MS), and the obtained ion chromatograms were analyzed using NIST 08 database. The expressions of related genes were analyzed by fluorescence quantitative PCR.【Result】After ethylene treatment, the emissions of volatile scent compounds ocimene, linalool, allo-ocimene, β-caryophyllene and α-farnesene increased significantly, and the expressions of related terpene synthase genes HcTPS1, HcTPS3 and HcTPS10 increased by 116%, 182% and 63% respectively. After 1-MCP treatment, the emissions of ocimene, allo-ocimene and α-farnesene decreased significantly, and the expressions of HcTPS1, HcTPS3 and HcTPS10 decreased by 45%, 30% and 30% respectively. After ethylene treatment, the expressions of nuclear transcription factors HcEIL1-1 and HcEIL1-2 increased by 1.51 and 2.14 times respectively. After 1-MCP treatment, the expressions of HcEIL1-1 and HcEIL1-2 decreased by 30% and 60% respectively.【Conclusion】Exogenous ethylene can increase the emission of terpenoids in H. coronarium. The expression patterns of HcEIL1-1 and HcEIL1-2, the nuclear transcription factors in ethylene signaling pathway, were consistent with the expression pattern of terpenoid synthase genes.
LI Qingying , ZHONG Chonglu , JIANG Qingbin , ZHANG Yong , CHEN Yu , WEI Yongcheng , CHEN Zhen
2018, 39(4):73-79. DOI: 10.7671/j.issn.1001-411X.2018.04.012
Abstract:【Objective】To understand genetic variation characteristics and provide bases for superior families selection and germplasm resources reasonable exploitation and utilization in Michelia macclurei based on estimation of wood property genetic parameters among families and correlation analyses between wood properties and growth traits.【Method】Genetic variation characteristics of wood basic density, fiber length, fiber width and fiber length/width ratio were analyzed using 100 families in M. macclurei provenance family forest.【Result】Timber characteristics among families existed significant differences (P<0.01) and had great selection potentials. The variation ranges of wood basic density, fiber length, fiber width and fiber length/width ratio among different provenances were 0.38-0.53 g·cm-3、0.65-0.91 mm、23.20-28.87 μm and 22.92-38.66 respectively, with mean values of 0.48 g·cm-3、0.79 mm、25.88 μm and 30.74 respectively. The genotypic and phentypic correlation analyses showed that the wood properties of each family were significantly correlated with the growth traits, except no significant genotypic correlation between fiber length and diameter at breast height or volume. The correlation between wood density and fiber length was not significant, and these two traits could be selected independently. The individual heritability of wood basic density and fiber length were 0.374 and 0.372 respectively, both under highly genetic control. The individual heritability of fiber width and fiber length/width ratio were 0.166 and 0.231 respectively, both under moderate genetic control.【Conclusion】The superior wood property families are selected through intense and moderate selection methods. In consideration of actual production and following breeding, the moderate selection is more suitable for the present experimental forest. This study selected 52 superior wood basic density families, 44 superior fiber length families, and 23 shared families. These 23 families are more suitable as suporior materials for combined primary selection.
WANG Yi , WANG Xuekai , ZHOU Wei , YANG Fuyu , ZHANG Qing , CHEN Xiaoyang
2018, 39(4):80-86. DOI: 10.7671/j.issn.1001-411X.2018.04.013
Abstract:【Objective】To investigate the effects of different moisture contents and additives on ensiling fermentation qualities of Neolamarckia cadamba leaves.【Method】The N. cadamba leaves were used as raw materials, and air-cured until the moisture contents (w) were 80% and 70% respectively. The ensiling processes were conducted through adding formic acid (FA, 3 g·kg-1), Lactobacillus plantarum (LP, 1×106 cfu·g-1) and Zhuanglemei silage starter (ZLM, 0.005 g·kg-1) to each moisture content treatment. The treatment without additive was counted as the control (CK).【Result】The crude protein content (w) of N. Cadamba leaves without air-curing before ensiling was 22.98%, and the neutral and acid detergent fiber contents (w) were 32.84% and 24.91% respectively. The moisture content had significant effects on silage qualities of N. cadamba leaves. With the decrease of moisture content, the dry matter and water soluble carbohydrate contents increased extremely significantly (P<0.01), pH decreased extremely significantly (P<0.01). Under the two moisture contents, the pH of the treatments adding LP and ZLM were extremely significantly lower than CK (P<0.01). While the moisture content was 70%, two lactobacillus starters (LP and ZLM) extremely significantly reduced the numbers of yeast (P<0.01). The water soluble carbohydrate content in the treatment adding FA under 70% moisture content was extremely significantly higher than those in CK and other treatments (P<0.01). The logarithm values of Escherichia coli numbers in all treatments were less than 2.0, no butyric acid was detected, and all rations of w(NH4+-N) to w(TN) were below 10%.【Conclusion】N. cadamba leaves have high feeding value and are liable to produce silage. Reducing moisture content properly and applying additives can partly improve the ensiling fermentation qualities of N. cadamba leaves.
FANG Yiran , CAI Jinhuan , XUE Li
2018, 39(4):87-92. DOI: 10.7671/j.issn.1001-411X.2018.04.014
Abstract:【Objective】To investigate the undergrowth diversity and soil physical properties in three plantations at Yunyong forest farm in Foshan, Guangdong Province and provide a theoretical basis for ecological forest cultivation and management.【Method】Tutcheria championii was planted in a pure broad-leaved plantation (P1 plot); Cunninghamia lanceolata, Mytilaria laosensis, Elaeocarpus apiculatus, Michelia macclurei, Liquidambar formosana and Alstonia scholarisv were planted in a mixed conifer-broad leaf plantation (P2 plot); M. laosensis, Castanopsis hystri and Erythrophleum fordii were planted in a mixed broad-leaved plantation (P3 plot). Species diversity, coverage, number of total plants and average height of shrub-herb layer under canopy of above plots were investigated. Diversity and evenness indexes were used to study undergrowth species diversity of these plantations. The 100 cm3 cutting ring was used to collect soil samples from the 0-20 cm soil layer at three plots, respectively, and soil physical properties such as water content and bulk density were determined.【Result】The mean values of Shannon-Wiener diversity index and Simpson diversity index of shrub species were lower than herb species while the mean values of evenness indexes of shrub species were larger than herb species in the three plots. The growth status of herb species were superior to shrub species in the three plots. The species number and quantity of the undergrowth of P1 plot were more than those of P2 and P3 plots. The soil bulk density of P1 plot was the highest among the three plots while that of P2 plot was the lowest. The values of total soil porosity, natural moisture content, capillary moisture capacity, capillary porosity, non-capillary porosity and aeration porosity of the three plots were all in the order of P2 plot > P3 plot > P1 plot.【Conclusion】The community structure and soil quality of mixed plantations are better than those of pure plantation.
LI Jiawei , CHEN Yan , WANG Jiasheng , LIU Weiwei , ZENG Zeqin , ZOU Xiangjun
2018, 39(4):93-99. DOI: 10.7671/j.issn.1001-411X.2018.04.015
Abstract:【Objective】To develop an automatic grading machine of litchi.【Method】An automatic monomeric delivery device was designed and a delivery experiment was performed. The influences of different working and structural parameters on the stuck frequency and cavity rate of litchi were analyzed. Regression and combined models were established. Influencing factors were optimized comprehensively.【Result】Both stuck frequency and cavity rate increased with the rotation speed of big turnable, the cycle length of small turnable and the feeding volume increasing. Both stuck frequency and cavity rate increased firstly and then decreased with the number of holes of big turnable increasing. The cavity rate also increased with the offset coefficient of amplitude of small turnable increasing. The major factor influencing the stuck frequency and cavity rate was the feeding volume, followed by the rotation speed of big turnable, then the offset coefficient of amplitude of small turnable. The optimial parameter combination from the combined regression model was better than those from the orthogonal experiment and the independent regression model. The optimial parameters of the delivery device were as follows: the rotation speed of big turnable was 7.35 r·min-1, the rotation amplitude of small turnable was 14.30 r·min-1, the cycle length of small turnable was 16.93 s, the offset coefficient of amplitude of small turnable was 0.33, the rotation motion of small turnable changed linearly, the feeding volume was 1.31 kg and the number of holes of big turnable was 10. Based on this combination, the stuck frequency was 0 and the cavity rate was 6%.【Conclusion】The results can provide references for the designation of automatic grading and monomeric delivery device of litchi.
ZHAO Bo , WANG Xiaochan , YANG Sijun , Morice O. ODHIAMBO
2018, 39(4):100-104. DOI: 10.7671/j.issn.1001-411X.2018.04.016
Abstract:【Objective】To solve the problem that when rice stubble cleaner operates, its driving wheels easily roll down high remaining stubbles, causing many lodged stubbles on the wheel track and poor crushing effect.【Method】A rice stubble cleaner with lifting function was developed in this paper. This machine adopted L-shaped rake finger of stalk lifter fixed on the stubble-lifting shaft, and a shunting transmission mode of stubble-lifting shaft and crushing shaft on both sides, so that the front stubble-lifting system and the rear crushing system cooperated with each other to enable lodged remaining stubbles to be lifted and immediately put into the crushing chamber. The field performance of this stubble cleaner was tested based on an orthogonal design.【Result】The forward speed, rotational speed of roller and width of lifting finger of rice stubble cleaner had significant impact on stubble crushing rate. The optimum combination of working parameters of this equipment was that the forward speed was 5.38 km·h-1, the rotational speed of roller was 160 r·min-1 and the width of lifting finger was 8 cm. The crushing effect of this combination was the best and crushing rate reached 92.52%.【Conclusion】This lifting mechanism is simple and its lifting and crushing effects are good. This stubble cleaner can improve crushing efficiency of high remaining rice stubbles in actual production.
CHENG Xijun , ZHU Huibin , BAI Lizhen , GUO Zihao , WANG jie , AN Ran , CAO Kegao
2018, 39(4):105-113. DOI: 10.7671/j.issn.1001-411X.2018.04.017
Abstract:【Objective】To design a disc-cutter type no-till maize seeder equipped with a micro tillage machine, and solve the problems that the no-till seeder has difficulties in passing through the field and has unsatisfactory seeding quality in the southwest area of China with heavy clay soil and relatively small field area.【Method】Disc knife and walking device with depth wheel were applied to break stubble and ditch, limit depth and prevent skidding. The effects of flat, corrugated and notched disc knife operations on stubble breaking, traction resistance and fuel consumption were investigated. Soil cut with three types of cutting discs was simulated by LS-DYNA and the key parameters were designed.【Result】The field test showed that the soil disturbance quantity and fuel consumption of the no-tillage seeder equipped with flat cutting disc were about 4.03% and 14.64% lower than those of the corrugated type, respectively, and 1.15% and 7.51% lower than those of the notched type. The traction resistance reached the minimum value of 1.2 kN at the speed of 0.54 m·s–1. The qualified rates of seeding and fertilizing depth with three types of cutting discs were above 85% and the qualified index of seed spacing was 90.3%.【Conclusion】This study provide the design basis for the no-tillage seeder with disc cutter in the southwest of China.
2018, 39(4):114-119. DOI: 10.7671/j.issn.1001-411X.2018.04.018
Abstract:【Objective】To improve the intelligence and safety of remote-operated agricultural vehicles. 【Method】A new method for unmanned agricultural vehicles during obstacle crossing was proposed. The collision prediction model of unmanned vehicles in dynamic environment was established, and the real-time collision location was determined. According to the cloud inference principle based on both experience of experts and agricultural operation environment, velocity control strategy was established to realize velocity control. 【Result】The algorithm took 0.170 1 s on average to make a prediction, the velocity control results of unmanned vehicles excluded the impact of non-threatening obstacles and accorded with the velocity cloud inference principle.【Conclusion】The established algorithm is able to realize real-time collision prediction, possesses anti-disturbance ability, and satisfies real-time requirement.
GU Jin , ZHANG Weiwei , GUAN Litao , HU Chuanshuang
2018, 39(4):120-124. DOI: 10.7671/j.issn.1001-411X.2018.04.019
Abstract:回顾国内外纤维素纳米材料的研究发展历程,对近年来国际上纤维素纳米材料相关标准化工作现状进行总结,分别介绍了纤维素纳米材料分类与命名、表征手段、商品化和环境、健康与安全等方面的相关标准,包括已经完成和正在进行的标准研制工作。根据国际上纤维素纳米材料的标准工作进展,提出制定我国纤维素纳米材料标准工作的建议和设想。
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